Diogo Thimoteo da Cunha
Federal University of São Paulo
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Featured researches published by Diogo Thimoteo da Cunha.
Food Research International | 2017
Laís Mariano Zanin; Diogo Thimoteo da Cunha; Veridiana Vera de Rosso; Vanessa Dias Capriles; Elke Stedefeldt
This study presents an overview of the relationship between knowledge, attitudes and practices (KAP) of food handlers with training in food safety, in addition to proposing reflections on the training of food handlers, considering its responsibility for food safety and health of consumers. The review was based on the integrative method. The descriptors used were: (food handler), (knowledge, attitudes and practice) and (training). Six databases were searched, 253 articles were consulted and 36 original articles were included. Fifty per cent of the articles pointed that there was no proper translation of knowledge into attitudes/practices or attitudes into practices after training. Knowledge, attitudes and practices of food handlers are important for identifying how efficient training in food safety is allowing prioritize actions in planning training. The evaluation of KAP is the first step to understand the food handlers point of view. After this evaluation other diagnostic strategies become necessary to enhance this understanding.
Ciencia & Saude Coletiva | 2013
Elke Stedefeldt; Diogo Thimoteo da Cunha; Eneo Alves da Silva Junior; Sueli Maria da Silva; Ana Beatriz Almeida de Oliveira
The scope of this study was to elaborate and validate an assessment tool for Best Practices in School Food and Nutrition Units. For this purpose a survey was conducted in the city of Santos (Sao Paulo - Brazil), in two stages: 1) Preparation of a Checklist for Best Practices in School Food and Nutrition Units (BPAE checklist). 2) Validation of the BPAE checklist. This checklist was applied in 76 public schools along with two other reference checklists. Sensitivity and specificity were estimated using the Receiver Operating Characteristics (ROC) Curve and the checklist was evaluated by application of the Likert scale. The thematic groups received weights to calculate the percentage of adequacy. After the application of the checklists in schools, the area under the curve value of 0.79 was found for the SS-196 checklist and 0.85 for the 542/2006 administrative checklist. These values indicate good suitability of the BPAE checklist to identify inadequacies, and it also received a positive assessment from the school food nutritionists. It proved that the checklist will assist in decision-making to ensure quality control. Once the BPAE checklist has been fully tested and validated it should prove an important tool for quality control in school nutrition.
Frontiers in Microbiology | 2016
Diogo Thimoteo da Cunha; Ana Lúcia de Freitas Saccol; Eduardo Cesar Tondo; Ana Beatriz Almeida de Oliveira; Veronica Cortez Ginani; Carolina V. Araújo; Thalita A. S. Lima; Angela Karinne Fagundes de Castro; Elke Stedefeldt
In 2014, Brazil hosted one of the most popular sport competitions in the world, the FIFA World Cup. Concerned about the intense migration of tourists, the Brazilian government decided to deploy a food safety strategy based on inspection scores and a grading system applied to food services. The present study aimed to evaluate the results of the food safety strategy deployed during the 2014 FIFA World Cup in Brazil. To assess food safety, an evaluation instrument was applied twice in 1927 food service establishments from 26 cities before the start of the competition. This instrument generated a food safety score for each establishment that ranged from 0.0 (no flaws observed) to 2565.95, with four possible grades: A (0.0–13.2); B (13.3–502.6); C (502.7–1152.2); and pending (more than 1152.3). Each food service received a stamp with the grade of the second evaluation. After the end of the World Cup, a study was conducted with different groups of the public to evaluate the acceptance of the strategy. To this end, 221 consumers, 998 food service owners or managers, 150 health surveillance auditors, and 27 health surveillance coordinators were enrolled. These participants completed a survey with positive and negative responses about the inspection score system through a 5-point Likert scale. A reduction in violation scores from 393.1 to 224.4 (p < 0.001) was observed between the first and second evaluation cycles. Of the food services evaluated, 38.7% received the A stamp, 41.4% the B stamp, and 13.9% the C stamp. All positive responses on “system reliability” presented a mean of 4.0 or more, indicating that the public believed this strategy is reliable for communicating risks and promoting food safety. The strategy showed positive results regarding food safety and public acceptance. The deployed strategy promoted improvements in the food safety of food services. The implementation of a permanent policy may be well accepted by the public and may greatly contribute to a reduction in foodborne diseases (FBDs).
Revista De Nutricao-brazilian Journal of Nutrition | 2014
Diogo Thimoteo da Cunha; Hélida Ventura Barbosa Gonçalves; Aline Fátima Andrade de Lima; Paula Andrea Martins; Veridiana Vera de Rosso; Elke Stedefeldt
Objective: the objective of this study was to evaluate the nutritional composition and describe the acceptability of regional culinary dishes served to students from public schools of rural and urban areas. Methods: Ten Brazilian regional dishes were evaluated for acceptability and nutritional composition. the survey was conducted in schools located in rural and urban areas of two cities in the state of Sao Paulo. Dish acceptability was evaluated using leftover analysis and a 5-point facial hedonic scale. the adherence index was calculated and used as an indirect measure of acceptance, and the nutritional composition was calculated based on the technical files of each dish. Results: A total of 2,384 students from 20 schools participated in the study and 1,174 tasted and evaluated the dishes. the test using the 5-point facial hedonic scale demonstrated that five dishes (Caldo verde soup, persimmon jelly, chicken with okra, pureed cornmeal with spinach, and arugula pizza) had an acceptability rate above 85.0%. the mean adherence indices were 57.3% and 55.6% in urban and rural environments, respectively. Analysis of the nutritional composition of regional dishes indicates that these dishes can partially meet macronutrient recommendations. Conclusion: the tested dishes can become part of school menus as they were accepted or partly accepted by the students regardless of school location, whether rural or urban. the cultural heritage is an important resource for the food sovereignty of a country and should be constantly encouraged.
Revista chilena de nutrición | 2013
Diogo Thimoteo da Cunha; Raquel Braz Assunção Botelho; Rafaela Ribeiro de Brito; Lívia de Lacerda de Oliveira Pineli; Elke Stedefeldt
The objective of this study was to validate methods to assess the acceptability on scholar feeding. A total of 354 students took part in this survey, from second to fiyears of public education system in Santos city, Sao Paulo, Brazil. A comparison between three methods was used: mixed facial hedonic scale of fi points, playful cards and leftover analysis that was the reference method. Kappa statistics and quadratic kappa were used to verify the concordance between the methods. There was an estimated reasonable agreement between the leftover analysis and the affective methods: mixed facial hedonic scale (kappa = 0.2414) and playful cards (kappa=0.2758), all of them with p<0,001. When compared the mixed facial hedonic scale and the playful cards, it was observed a quadratic kappa of 0.3846. It is recommended that the application of the validated methods in the school context be studied in different spheres of government and in other countries as well.
Journal of Food Protection | 2016
Diogo Thimoteo da Cunha; Veridiana Vera de Rosso; Elke Stedefeldt
The objective of this study was to verify the characteristics of food safety inspections, considering risk categories and binary scores. A cross-sectional study was performed with 439 restaurants in 43 Brazilian cities. A food safety checklist with 177 items was applied to the food service establishments. These items were classified into four groups (R1 to R4) according to the main factors that can cause outbreaks involving food: R1, time and temperature aspects; R2, direct contamination; R3, water conditions and raw material; and R4, indirect contamination (i.e., structures and buildings). A score adjusted for 100 was calculated for the overall violation score and the violation score for each risk category. The average violation score (standard deviation) was 18.9% (16.0), with an amplitude of 0.0 to 76.7%. Restaurants with a low overall violation score (approximately 20%) presented a high number of violations from the R1 and R2 groups, representing the most risky violations. Practical solutions to minimize this evaluation bias were discussed. Food safety evaluation should use weighted scores and be risk-based. However, some precautions must be taken by researchers, health inspectors, and health surveillance departments to develop an adequate and reliable instrument.
British Food Journal | 2014
Diogo Thimoteo da Cunha; Elke Stedefeldt; Veridiana Vera de Rosso
Purpose – The aim of this study was to evaluate the good manufacturing practices in meal services in public schools and day care centres in Baixada Santista, Brazil using a health risk classification. Design/methodology/approach – A total of 59 public schools and day care centres in nine municipalities of Baixada Santista – Brazil were randomly selected. The sanitary conditions were evaluated by applying a validated checklist specific to school meal service. The questions were scored according to the health risks detected. A percentage of conformity to good manufacturing practices was calculated, than schools and day care centres were classified according to health risk in relation to the scores obtained for each thematic area addressed in the checklist and in relation to the overall score. Findings – It was observed that 62 per cent of the schools were classified as average health risk failing to comply with Brazilian legislation on food safety rules. The thematic areas that showed lower percentage of co...
Ultrasonics Sonochemistry | 2018
Beatriz Sumere; Mariana Corrêa de Souza; Mariana Pacífico dos Santos; Rosângela Bezerra; Diogo Thimoteo da Cunha; Julian Martínez; Mauricio A. Rostagno
The combination of ultrasound and pressurized liquid extraction (UAPLE) was evaluated for the extraction of phenolic compounds from pomegranate peels (Punica granatum L.). The influence of several variables of the process on extraction yield, including solvent type (water, ethanol + water 30, 50 and 70% v:v), temperature (50-100 °C), ultrasound power (0-800 W at the generator, or 0-38.5 W at the tip of the probe), mean particle size (0.68 and 1.05 mm), and number of cycles (1-5), were analyzed according to the yield of 20 different phenolic compounds. The most suitable temperatures for the extraction of phenolic compounds using water were from 70 to 80 °C. In general, 100 °C was not adequate since the lowest extraction yields were observed. Results suggested that ultrasound had a greater impact on extraction yields using large particles and that intermediate ultrasound power (480-640 W at the generator, or 23.1-30.8 W at the tip of the probe) produced the best results. Using small particles (0.68 mm) or large particles (1.05 mm), extraction with ultrasound was 1 cycle faster. Ultrasound may have offset the negative effect of the use of large particles, however, did not increase the yield of phenolic compounds in any of the cases studied after five cycles. Additionally, the continuous clogging problems observed with small particles were avoided with the use of large particles, which combined with ultrasound allowed consistent operation with good intra and inter-day reproducibility (>95%). Using samples with large particle size, the best extraction conditions were achieved with water extraction solvent, 70 °C extraction temperature, ultrasound power at 480 W, and 3 cycles, yielding 61.72 ± 7.70 mg/g. UAPLE demonstrated to be a clean, efficient and a green alternative for the extraction of phenolic compounds from pomegranate peels. These findings indicate that UAPLE has a great potential to improve the extraction of bioactive compounds from natural products.
Food Safety#R##N#Emerging Issues, Technologies and Systems | 2015
Elke Stedefeldt; Laís Mariano Zanin; Diogo Thimoteo da Cunha; Veridiana Vera de Rosso; Vanessa Dias Capriles; Ana Lúcia de Freitas Saccol
Abstract It is well known that participation in training programs tends to improve food handlers knowledge. However, several factors can prevent the transformation of food safety knowledge into positive attitude and adequate practices. Among these factors we find the risk/benefit perceptions, perceived behavioral control and intention, motivation, leadership, and other psychological factors. Understanding the relationship of these factors can provide guidance to plan more effective food safety training. The food safety laws and standards in several countries, including Brazil, list the topics to be addressed in training of food handlers. However, the laws and standards do not establish clear guidelines. This chapter aspires to discuss different strategies to evaluate food handlers and practices to plan strategies applied to handlers in food chain production. Such discussion aims to present the weaknesses and strengths in food safety quality control, covering the role of trainers, stakeholders, the laws, and food safety standards.
Journal of Food Quality | 2018
Vanessa Vanderléia Merlini; Fabíola de Lima Pena; Diogo Thimoteo da Cunha; Julicristie Machado de Oliveira; Mauricio A. Rostagno; Adriane Elisabete Costa Antunes
The aim of this study was to assess the microbiological profile of leafy vegetables from organic and conventional farming ( ). The microbiological parameters analyzed consisted of aerobic mesophilic bacteria, yeasts and molds, coliforms at 30 and 45°C, and Salmonella ssp. A biochemical identification of the Enterobacteriaceae species was carried out. Some Enterobacteriaceae species were identified in the produce from both farming systems and Hafnia alvei was the most widespread specie observed. Salmonella spp. was not detected in the samples analyzed. The microbial counts for indicator microorganisms were, in general, higher for conventional leaves when compared to those produced by organic farming.
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Ana Beatriz Almeida de Oliveira
Universidade Federal do Rio Grande do Sul
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