Dipti B. Shah

Homology Modelling of Conserved rbcL Amino Acid Sequences in Leguminosae Family

This study is focus on Homology modelling of few Leguminosae family species which are found in Gujarat state, INDIA. There are three subfamilies of Leguminosae family which are Fabaceae (Papilionaceae), Caesalpiniaceae and Mimosaceae. Multiple sequence alignment carried out of few species’ rbcL protein sequences in each subfamily and conserved amino acid considered for homology modelling. Evolutionarily related proteins have similar sequences and naturally occurring homologous proteins have similar protein structure. It has been shown that three-dimensional protein structure is evolutionarily more conserved than would be expected on the basis of sequence conservation alone; we found that there are few amino acids which are common with same base pairs in each sub-family even though they are from different genus. There is no Protein structure available of conserved amino acids in PDB database of our study so we did homology modelling of three rbcL protein sequences (one from each sub family) which are found conserved in Multiple sequence alignment and structure validation with Ramachandran Plot was carried out and CASTp server was used to find out active sites in predicted protein structure and finally function of each predicted protein reported after this homology modeling of few conserved rbcL amino acid sequences in Leguminosae family.

古吉拉特邦豆科家庭数据库(GLDB)：存在于印度古吉拉特邦的豆科植物生物信息学数据库

生物数据库在生物信息学中发挥着重要的作用，它们为科学家提供了集中访问各种生物数据的机会。目前，分子数据可用于对许多不同植物物种进行分类、进化和情缘关系分析。我们尝试为豆科家族物种生成初步的生物信息学数据，尽可能在一种平台上提供特定类型的信息。总之，我们做了一个包括在印度的古杰雷特州发现的豆科家族所有信息的数据库，包含每个物种的植物学信息和生物信息学信息，并在一个平台上进行分析。创建这种类型的数据库反映出生物信息学可以在植物数据库中发挥重要作用的当代的跨学科方法。开发生物信息学数据库并将所有这些相关信息放在社会特别是科学界一个广泛的平台上，使之得到进一步延伸。