Dmytro Kornyeyev

Soil bacteria augment Arabidopsis photosynthesis by decreasing glucose sensing and abscisic acid levels in planta

Photosynthesis is regulated by environmental factors as well as endogenous sugar signals. Whereas light-driven sugar biosynthesis is essential for terrestrial organisms, as well as belowground microflora, whether and how soil symbionts regulate photosynthesis has yet to be reported. Here, we show that the plant growth-promoting soil bacterium Bacillus subtilis GB03 augments photosynthetic capacity by increasing photosynthetic efficiency and chlorophyll content in Arabidopsis. Mechanistic studies reveal an elevation of sugar accumulation as well as the suppression of classic glucose signaling responses, including hypocotyl elongation and seed germination, with exposure to GB03. Compared with wild-type plants, two Arabidopsis mutants defective in hexokinase-dependent sugar signaling exhibit increased photosynthetic capacity, which is not further enhanced with GB03 exposure. Overlap in sugar/ABA sensing is observed in GB03-exposed plants, with a reduction of ABA-biosynthetic transcripts as well as downstream metabolite levels in leaves. Moreover, exogenous ABA abrogates GB03-triggered increases in photosynthetic efficiency and chlorophyll content. These results demonstrate that certain rhizobacteria elevate photosynthesis through the modulation of endogenous sugar/ABA signaling, and establish a regulatory role for soil symbionts in plant acquisition of energy.

The role of antioxidant enzymes in photoprotection

The enzymatic component of the antioxidant system is discussed as one of the defensive mechanisms providing protection against excessive light absorption in plants. We present an analysis of attempts to improve stress tolerance by means of the creation of transgenic plants with elevated antioxidant enzyme activities and conclude that the effect of such transgenic manipulation strongly depends on the manner in which the stress is imposed. The following factors may diminish the differences in photosynthetic performance between transgenic plants and wild type under field conditions: effective functioning of the thermal dissipation mechanisms providing a primary line of defense against excessive light, long-term adjustments of the antioxidant system and other photoprotective mechanisms, the relatively low level of control over electron transport exerted by the Water–Water cycle, especially under warm conditions, and a decrease in the content of the transgenic product during leaf aging.

Elevated chloroplastic glutathione reductase activities decrease chilling-induced photoinhibition by increasing rates of photochemistry, but not thermal energy dissipation, in transgenic cotton

The effect of the overproduction of glutathione reductase (GR+) in cotton (Gossypium hirsutum L. cv.Coker 312) chloroplasts on the response of photosynthetic parameters to chilling in the light was examined. After 180 min at 10°C and 500 μmol photons m-2 s-1 in the chamber of an oxygen electrode, leaf discs of GR+ plants exhibited lower levels of sustained PSII photoinhibition than leaf discs of wild-type plants. No genotypic differences in thermal energy dissipation, leaf pigment composition, or the dynamics of xanthophyll cycle de-epoxidation were observed. The rate of induction and steady-state levels of photochemistry were greater for GR+ in comparison to wild-type plants. Enhanced photochemistry in GR+ plants could not be attributed to higher rates of CO2 assimilation at 10°C. Although GR overproduction afforded some increased protection against PSI photoinactivation, suggesting improved scavenging of reactive oxygen species, higher PSI activities could not completely explain the greater rates of photochemistry. Pools of glutathione and ascorbate were significantly more reduced in GR+ plants. Increased demand for reducing power to maintain these constituents in the reduced state may contribute to the higher rates of photochemistry observed in GR+ plants.

Energy partitioning in photosystem II complexes subjected to photoinhibitory treatment

Chlorophyll a fluorescence measured in vivo is frequently used to study the role of different processes influencing the distribution of excitation energy in PSII complexes. Such studies are important for understanding the regulation of photosynthetic electron transport. However, at the present time, there is no unified methodology to analyse the energy partitioning in PSII. In this article, we critically assess several approaches recently developed in this area of research and propose new simple equations, which can be used for de-convolution of non-photochemical energy quenching in PSII complexes.

Transgenic overproduction of glutathione reductase does not protect cotton, Gossypium hirsutum (Malvaceae), from photoinhibition during growth under chilling conditions

In some studies, tissues from plants that have been genetically transformed to overproduce antioxidant enzymes sustain less damage when abruptly exposed to short-term chilling in the laboratory. However, few studies have examined the performance of transgenic plants during longer-term growth under chilling conditions. We compared growth of transgenic cotton that overproduces glutathione reductase (GR+; ∼40-fold overproduction) to growth of the wild type in a controlled environment chamber as leaf temperature was lowered from 28° to 14°C over 9 d and for a subsequent 9-d period at 14°C. In wild-type and GR+ cotton, chilling temperatures resulted in decreased dark-adapted F(v)/F(m) (the ratio of variable to maximal fluorescence; a measure of maximum photosystem II quantum yield) and mid-light period photosystem II quantum yield, coupled with increased 1 - q(P) (a nonlinear estimate of the reduction state of the primary quinone acceptor of photosystem II). The capacity for photosynthetic oxygen evolution decreased during the first portion of the chilling exposure, but recovered slightly during the second half. At no point during the chilling exposure did the performance of GR+ plants differ significantly from that of wild-type plants in any of the above parameters. The absence of an effect of GR overproduction under longer-term chilling may be explained, in part, by the fact that wild-type cotton acclimated to chilling by upregulating native GR activity.