Dolores M. Pirnik

Orevactaene,1 a novel binding inhibitor of HIV-1 rev protein to Rev response element (RRE) from Epicoccum nigrum WC47880

Orevactaene (1), a novel oxopolyene, was isolated from Epicoccum nigrum WC47880 during the screening of microbial fermentation extracts for their ability to inhibit the binding between HIV-1 regulatory protein Rev and its viral RNA-binding site, Rev response element (RRE). The structure of 1 was elucidated by spectroscopic methods. Compound 1 displayed inhibitory activity against the Rev/RRE binding with an IC50 value of 3.6 μM.

1-Methoxy-agroclavine from Penicillium sp. WC75209, a novel inhibitor of the Lck tyrosine kinase

A high-throughput screen was developed and implemented to identify inhibitors of the Lck tyrosine kinase. This report describes the identification of a specific inhibitor of this enzyme from the solid fermentation culture of the Penicillium sp., WC75209. The active compound was isolated and structurally characterized as 1-methoxy-5R, 10S-agroclavine, a new member of the ergot alkaloid family.