Domenico Tegolo

VAMPIRE: Vessel assessment and measurement platform for images of the REtina

We present VAMPIRE, a software application for efficient, semi-automatic quantification of retinal vessel properties with large collections of fundus camera images. VAMPIRE is also an international collaborative project of four image processing groups and five clinical centres. The system provides automatic detection of retinal landmarks (optic disc, vasculature), and quantifies key parameters used frequently in investigative studies: vessel width, vessel branching coefficients, and tortuosity. The ultimate vision is to make VAMPIRE available as a public tool, to support quantification and analysis of large collections of fundus camera images.

Automated Detection of Optic Disc Location in Retinal Images

This contribution presents an automated method to locate the optic disc in color fundus images. The method uses texture descriptors and a regression based method in order to determine the best circle that fits the optic disc. The best circle is chosen from a set of circles determined with an innovative method, not using the Hough transform as past approaches. An evaluation of the proposed method has been done using a database of 40 images. On this data set, our method achieved 95% success rate for the localization of the optic disc and 70% success rate for the identification of the optic disc contour (as a circle).

Accurate estimation of retinal vessel width using bagged decision trees and an extended multiresolution Hermite model

We present an algorithm estimating the width of retinal vessels in fundus camera images. The algorithm uses a novel parametric surface model of the cross-sectional intensities of vessels, and ensembles of bagged decision trees to estimate the local width from the parameters of the best-fit surface. We report comparative tests with REVIEW, currently the public database of reference for retinal width estimation, containing 16 images with 193 annotated vessel segments and 5066 profile points annotated manually by three independent experts. Comparative tests are reported also with our own set of 378 vessel widths selected sparsely in 38 images from the Tayside Scotland diabetic retinopathy screening programme and annotated manually by two clinicians. We obtain considerably better accuracies compared to leading methods in REVIEW tests and in Tayside tests. An important advantage of our method is its stability (success rate, i.e., meaningful measurement returned, of 100% on all REVIEW data sets and on the Tayside data set) compared to a variety of methods from the literature. We also find that results depend crucially on testing data and conditions, and discuss criteria for selecting a training set yielding optimal accuracy.

A distributed architecture for autonomous navigation of robots

The paper shows a distributed architecture for autonomous robot navigation. The architecture is based on three modules that are implemented on separate and interacting agents: the target recognizer, the obsta90cle evaluator and the planner. An adaptive genetic algorithm has been studied to identify mechanisms for reaching the target and for manipulating the 2-directions of the robot; the distributed architecture has been embedded in the DAISY (Distributed Architecture for Intelligent System). Experiments have been carried out using a LEGO intelligent brick.

An automated image analysis methodology for classifying megakaryocytes in chronic myeloproliferative disorders

This work describes an automatic method for discrimination in microphotographs between normal and pathological human megakaryocytes and between two kinds of disorders of these cells. A segmentation procedure has been developed, mainly based on mathematical morphology and wavelet transform, to isolate the cells. The features of each megakaryocyte (e.g. area, perimeter and tortuosity of the cell and its nucleus, and shape complexity via elliptic Fourier transform) are used by a regression tree procedure applied twice: the first time to find the set of normal megakaryocytes and the second to distinguish between the pathologies. The output of our classifier has been compared to the interpretation provided by the pathologists and the results show that 98.4% and 97.1% of normal and pathological cells, respectively, have testified an excellent classification. This study proposes a useful aid in supporting the specialist in the classification of megakaryocyte disorders.

Shape analysis for image retrieval

The main aim of this paper is to describe a method for locating a subimage of a stored image that approximately matches a given query image. This matching can support naive users in accessing an image database according to image contents rather symbolic attributes. The query image can be either composed using painting tools or cuts out of an actual scanned image. Our method is based on the extraction of features from the query image and from the stored images. The following three steps are involved: (1) an ISODATA algorithm is applied to segment (into region) both the query image and the stored images; (2) the normalized moment and geometrical features are computed from the segmented regions, and (3) a matching process is run on the resulting features to find those stored images which should be retrieved. The result is an ordered list of stored images or subimages from the database.

Novel VAMPIRE algorithms for quantitative analysis of the retinal vasculature

This paper summarizes three recent, novel algorithms developed within VAMPIRE, namely optic disc and macula detection, arteryvein classification, and enhancement of binary vessel masks, and their performance assessment. VAMPIRE is an international collaboration growing a suite of software tools to allow efficient quantification of morphological properties of the retinal vasculature in large collections of fundus camera images. VAMPIRE measurements are currently mostly used in biomarker research, i.e., investigating associations between the morphology of the retinal vasculature and a number of clinical and cognitive conditions.

Scratch detection and removal from static images using simple statistics and genetic algorithms

This paper investigates the removal of line scratches from old movies and gives a twofold contribution. First, it presents simple technique for detecting the scratches, based on an analysis of the statistics of the grey levels. Second, the scratch removal is approached as an optimisation problem, and it is solved by using a genetic algorithm. The method can be classified as a static approach, as it works independently on each single frame of the sequence. It does not require any a-priori knowledge of the absolute position of the scratch, nor an external starting population of chromosomes for the genetic algorithm. The central column of the line scratch once detected is changed with a conventional linear interpolation; this transformation is the starting point of the optimisation process.

Single neuron binding properties and the magical number 7

When we observe a scene, we can almost instantly recognize a familiar object or can quickly distinguish among objects differing by apparently minor details. Individual neurons in the medial temporal lobe of humans have been shown to be crucial for the recognition process, and they are selectively activated by different views of known individuals or objects. However, how single neurons could implement such a sparse and explicit code is unknown and almost impossible to investigate experimentally. Hippocampal CA1 pyramidal neurons could be instrumental in this process. Here, in an extensive series of simulations with realistic morphologies and active properties, we demonstrate how n radial (oblique) dendrites of these neurons may be used to bind n inputs to generate an output signal. The results suggest a possible neural code as the most effective n‐ple of dendrites that can be used for short‐term memory recollection of persons, objects, or places. Our analysis predicts a straightforward physiological explanation for the observed puzzling limit of about 7 short‐term memory items that can be stored by humans.

Automatic unsupervised segmentation of retinal vessels using self-organizing maps and K-means clustering

In this paper an automatic unsupervised method for the segmentation of retinal vessels is proposed. A Self-Organizing Map is trained on a portion of the same image that is tested and K-means clustering algorithm is used to divide the map units in 2 classes. The entire image is again input for the Self-Organizing Map, and the class of each pixel will be the class of the best matching unit on the Self-Organizing Map. Finally, the vessel network is post-processed using a hill climbing strategy on the connected components of the segmented image. The experimental evaluation on the publicly available DRIVE database shows accurate extraction of vessels network and a good agreement between our segmentation and the ground truth. The mean accuracy, 0.9459 with a standard deviation of 0.0094, is outperforming the manual segmentation rates obtained by other widely used unsupervised methods. A good kappa value of 0.6562 is inline with state-of-the-art supervised and unsupervised approaches.