Dominika Saniewska

Distribution of mercury in different environmental compartments in the aquatic ecosystem of the coastal zone of the Southern Baltic Sea.

The aim of this study was to characterize mercury (Hg) contamination in the coastal waters of the Southern Baltic Sea, and to investigate transformations of Hg in the initial links of the marine food chain. Concentrations of Hg in water, particulate matter, plankton and macrophytes at various stations in the coastal zone (a bay with restricted water exchange, near an industrial city, river mouths, and the open sea) were measured in 2006-2008. Hg concentrations observed in the Southern Baltic varied greatly, showing the highest average values in all environmental compartments near the river mouths. In shallow, sheltered parts of the gulf, where water exchange is restricted, Hg concentrations in the water and in macrophytes were elevated relative to those in the coastal zone of the deeper part of the bay and in the open Baltic. Distance to the river mouth, terrestrial runoff, and quantity and quality of organic matter were more important than seasonal variations in controlling Hg and HgSPM concentrations in water samples. Mercury concentrations in the surface microlayer at the air/sea interface were over 10 times higher than those in the bulk surface water. Concentrations of Hg in macrophytes in the winter were significantly higher than those in the warm seasons (spring, summer, autumn). This was probably the combined effect of higher availability of Hg in porewaters and leaf growth inhibition.

Factors influencing variability of mercury input to the southern Baltic Sea

The southern Baltic countries have been identified as significant sources of Hg into the sea. Are anthropogenic activities the sole source? How do meteorological parameters influence the deposition? Studies on input of Hg to the Baltic were conducted in 2008-2012 in the Polish coastal stations. The riverine load was found to depend directly or indirectly on the amount of precipitation and catchment type. Input of atmospheric Hg increased along with the number of precipitation episodes from remote maritime air masses, as well as with the number of days when continental air masses from regional (when domestic heating prevailed) and remote sources moved over the Baltic, during the heating season. During the non-heating season metal input was found to be proportional to episodes of rain from continental regional air masses and to the number of days under influence of continental and maritime air masses from regional sources.

Mercury in Precipitation at an Urbanized Coastal Zone of the Baltic Sea (Poland)

Wet deposition is an important source of metals to the sea. The temporal variability of Hg concentrations in precipitation, and the impact of air masses of different origins over the Polish coastal zone were assessed. Samples of precipitation were collected (August 2008–May 2009) at an urbanized coastal station in Poland. Hg analyses were conducted using CVAFS. These were the first measurements of Hg concentration in precipitation obtained in the Polish coastal zone. Since Poland was identified as the biggest emitter of Hg to the Baltic, these data are very important. In the heating and non-heating season, Hg concentrations in precipitation were similar. Hg wet deposition flux dominated in summer, when the production of biomass in the aquatic system was able to actively adsorb Hg. Input of metal to the sea was attributed to regional and distant sources. Maritime air masses, through transformation of Hg(0), were an essential vector of mercury in precipitation.

Impact of intense rains and flooding on mercury riverine input to the coastal zone

The aim of the present research was to determine the impact of intense rains and flooding on mercury riverine input to the coastal zone. This study focused on four small rivers (Reda, Zagórska Struga, Płutnica, Gizdepka), typical of the Southern Baltic region, with no significant mercury sources. Samples were collected for 16months during average flow conditions and during selected meteorological events: floods, downpours, thaws and droughts. Results showed decreased retention of mercury during intense rainfalls, thus demonstrating mercury elution from the catchment. Floods and melting snow also have a tremendous impact on the outflow of mercury from the catchment. Development of urban infrastructure and farmlands increases the outflow of mercury from the catchment too, making such areas a significant source of mercury in the river. On the other hand, areas with natural character, predominated by forests, stimulate retention of mercury that reaches them through dry and wet atmospheric deposition.

Transfer of mercury and phenol derivatives across the placenta of Baltic grey seals (Halichoerus grypus grypus)

The placenta is an intermediary organ between the female and the developing foetus. Some chemical substances, including the most harmful ones, exhibit the ability to accumulate in or penetrate through the placenta. The aim of the study was to determine the role of the placenta of the Baltic grey seal (Halichoerus grypus grypus) in the transfer of endocrine disrupting compounds (EDCs) - (bisphenol A, 4-tert- octylphenol, 4- nonylphenol), as well as total and organic mercury. 30 placentas were collected from grey seals pupping under human care at the Hel Marine Station in the years 2007-2016. The assays were conducted using the technique of high-preformance liquid chromatography (phenol derivatives) and atomic absorption spectrometry (mercury and selenium). A measurable level of EDCs was indicated in the placentas of grey seals. It was established that the inorganic Hg form was accumulated in the placenta, and that its concentrations were an order of magnitude higher than the concentrations of the organic form, which penetrated to the foetus. Similar observations were made for phenol derivatives - bisphenol A, 4-tert- octylphenol and 4-nonylphenol. For this compound group the placenta was a barrier, but the properties of phenol derivatives suggest the possibility of their penetration through this organ.

Simple screening technique for determination of adsorbed and absorbed mercury in particulate matter in atmospheric and aquatic environment

The threat connected to mercury results from its capacity to be transported over long distances and its ability to bioaccumulate and biomagnify in the trophic chain, making it a global problem. Humans are situated at the top of the trophic ladder, and excess mercury manifests itself mainly in the onset of neurological conditions. The toxicity of mercury, as well as its residence time, depends on the form in which it occurs. However, analysis of mercury speciation is time-consuming and poses a high risk of additional or negative contamination. Hence, the mercury thermodesorption method, and particularly its use for fractionating Hg, offers many new possibilities. Here, the thermodesorption technique was applied to the determination of mercury fraction in particulate matter using a DMA-80 direct mercury analyser (Milestone, Italy). The presented method allows direct (without prior mineralisation) determination of labile and stabile mercury fractions within a relatively short time. Heating sample in subsequent temperatures enables determination the share of mercury adsorbed on the surface (mainly associated with halogenides (Hgads1) and HgSO4/HgO/HgF2 (Hgads2), as well as absorbed within the suspended particulate organic matter (Hgabs), in a relatively short time. This fractionation is of great importance in terms of estimating the transfer of mercury to and along the trophic chain. This method determines the contribution of two stable mercury fractions:: HgS and residual Hg, strongly bound to particulate matter matrix (Hgres). The novelty of this technique is also its joint ability to determine gaseous mercury bound to airboirne particulate matter, which will enable better understand Hg cycling in the atmosphere as well as mercury fraction in dry deposition flux. This method enables assessment of global mercury circulation in environment.

Mercury fractionation in marine macrofauna using thermodesorption technique: Method and its application

Mercury (Hg) is one of the most dangerous elements, and its toxicity and ability to accumulate in organisms depend on its chemical form. There are numerous methods of Hg speciation analysis, out of which the least expensive and the least time-consuming one is thermodesorption. The method has been successfully used for the analysis of abiotic samples - soils and sediments. The aim of this study was to verify whether the simplified thermodesorption method can be used in the analysis of the tissues of animal organisms from different trophic levels. Hg fractionation analyses were performed on a DMA-80 analyser (Milestone, Italy). The results presented in this paper are the first published data on Hg fractionation by thermodesorption method in animal tissues. The study showed that the 5-step thermodesorption method can be applied to various types of environmental matrices, which makes it universal. This method is of great importance in terms of estimating the Hg uptake and transfer in the trophic chain, and also enables the assessment of global Hg circulation in the environment. The presented method does not require previous digestion of samples or the use of expensive reagents. It can also be used for the preliminary selection of samples for MeHg analysis. The results obtained by this 5-step fractionation could be comparable with different research, conducted using other Hg analysers.

Watershed characteristics and climate factors effect on the temporal variability of mercury in the southern Baltic Sea rivers

Mercury (Hg) is a neurotoxic metal which can enter into the human organism mainly by fish consumption, skin and transpiration. In the coastal zone of the southern Baltic Sea, rivers are the main source of Hg. The Polish region represents the largest proportion of the Baltic Sea catchment and this research included four rivers of the Baltic watershed: the Reda, Zagórska Struga, Kacza and Gizdepka. The samples were collected in the years 2011-2013. Total and particulate Hg concentration in these rivers were measured. Due to intensive rain, deposited mercury on the catchment area was washed out into the riverines water and introduced into the Baltic Sea. Consequently, the load of Hg increased three times. Additionally, the intensive dry atmospheric deposition during heating season caused the increase of the concentration of particulate Hg in the river water even by 85%. The research confirmed the role of the river flow magnitude in the load of mercury introduced into the sea by rivers. Moreover, a high variability of mercury concentration was connected to the additional sources such as the chemicals containing Hg and no municipal sewage system. The analysis of stable isotopes indicated that the SPM contained terrestrial organic matter; however, there was no clear correlation between Hgtot, Corg and Ntot concentrations and δ13C, δ15N, C/N in particulate matter.

Inhalation - Route of EDC exposure in seabirds (Larus argentatus) from the Southern Baltic

Despite the presence of endocrine disrupting mercury, PAHs, alkylphenols and bisphenol A in inhaled air, scientific literature lacks information on their penetration into the lungs. Large lung capacity in birds makes this route of penetration more significant than in other animals. The studies were conducted on lungs of herring gulls found in the Gulf of Gdansk area. The results were juxtaposed with other tissues, including the intestines, which reflect the main, alimentary penetration route of harmful substances into the organism. It was determined that the capacity of birds lungs, affects the efficiency with which mercury is absorbed from the air. Birds found to have high mercury concentrations in lungs had low PAHs concentrations, what was determined by the fact that the birds foraged in two different areas, as well as on different trophic levels. The alimentary route of phenol derivatives into the organism was of greater significance than inhalation.