Dominique Chabasse

Molecular Mechanisms of Resistance to 5-Fluorocytosine in Laboratory Mutants of Candida glabrata

Resistance to 5-fluorocytosine (5-FC) has been poorly investigated in the yeast Candida glabrata. This study was conducted on laboratory mutants obtained by exposure of a wild-type isolate to 5-FC. Based on their susceptibility to 5-fluorouracil (5-FU), two of these mutants were selected for further analysis of the molecular mechanisms of 5-FC resistance. One mutant, resistant to both compounds, exhibited a missense mutation in the gene coding the cytosine deaminase and a decrease in the expression level of the gene coding the uridine monophosphate pyrophosphorylase. The other mutant that showed a reduced susceptibility to 5-FC and 5-FU exhibited an overexpression of the genes coding the thymidylate synthase and a cytosine permease, associated with a missense mutation in the last gene. Thus, beside mutations in the FUR1 gene which represent the most common cause of resistance to 5-FC, other mechanisms may also occur in C. glabrata.

Flore fongique des bacs à sable mise en evidence signification.

In order to evaluate the risk of fungal contamination in sand-boxes of the city of Angers game areas, the authors took 100 samples of sand from different environments: parks, gardens, buildings, nursery schools. They report the presence of a varied and important mycoflora and that the frequency and the distribution are more sensitive to the techniques used to isolate the fungus, as well as to the quality of the sand and to the eco-mesological factors in the four studied areas. The examination of earth samples has allowed the findings of a great number of keratinophilie species. The strains living in the sand do not represent a real danger of infection for the children.