Donna Snellgrove

In vitro and in vivo efficacy of garlic compounds against Gyrodactylus turnbulli infecting the guppy (Poecilia reticulata)

Traditional compounds used to treat fish diseases in aquaculture and the ornamental fish industry (such as formalin and malachite green) can be more toxic to the hosts than their parasites. With the reviviscence in the use of herbal products, various botanicals have been heralded as cures for particular pathogens, but the efficacy of these compounds for parasitic worms is questionable. Here, we tested a range of garlic (Allium sativum) products against a major aquarium pathogen, Gyrodactylus turnbulli, infecting the guppy (Poecilia reticulata). All garlic products significantly reduced parasite mean survival time in vitro, from 13 h to <1 h. In fully randomised trials, the number of parasites was also significantly reduced on infected fish exposed to garlic from different sources. Two garlic treatments (minced and granule forms) reduced worm burdens by 66% and 75% after three doses, whereas Chinese freeze-dried garlic and allyl disulphide were 95% effective after a single application. In fact, Chinese freeze dried garlic was equally effective as Levamisole, a licensed livestock dewormer that is highly effective against G. turnbulli but not routinely prescribed for use in fish; hence, garlic may be a potential alternative treatment for gyrodactylosis.

Mild maternal stress disrupts associative learning and increases aggression in offspring

Maternal stress has been shown to affect behaviour of offspring in a wide range of animals, but this evidence has come from studies that exposed gestating mothers to acute or severe stressors, such as restraint or exposure to synthetic stress hormones. Here we show that exposure of mothers to even a mild stressor reduces associative learning and increases aggression in offspring. Female guppies were exposed to routine husbandry procedures that produced only a minimal, non-significant, elevation of the stress hormone cortisol. In contrast to controls, offspring from mothers that experienced this mild stress failed to learn to associate a colour cue and food reward, and showed a greater amount of inter-individual variation in behaviour compared with control offspring. This mild stress also resulted in offspring that were more aggressive towards their own mirror image than controls. While it is possible that these results could represent the transmission of beneficial maternal characteristics to offspring born into unpredictable environments, the potential for mild maternal stress to affect offspring performance also has important implications for research into the trans-generational effects of stress.

Variation in the expression of dietary conservatism within and between fish species

When animals encounter new food, they must decide whether to eat it and risk being poisoned, or avoid it but risk losing valuable food resources. Some individuals within a foraging population are ‘adventurous consumers’ and readily accept novel food items into their diets, while others display an active and long-term avoidance of novel food, called ‘dietary conservatism’ (DC). Previous studies have shown the phenomenon of DC in many bird species and in a temperate fish, the three-spined stickleback, Gasterosteus aculeatus. However, it is unknown to what extent DC exists in other fish species, and so in the current study, we investigated the occurrence of DC in four species of tropical fish belonging to the poeciliid family (guppy, Poecilia reticulata, mollie, Poecilia sphenops, platy, Xiphophorus maculatus, and green swordtail, Xiphophorus hellerii). We detected DC strong enough to drive a novel prey morph from initial rarity to fixation in a prey population in all four poeciliid species. Despite some underlying differences in pre-existing preferences for prey colour, there was no significant difference between poeciliid species, sexes or sizes in the likelihood of the novel morph reaching fixation; neither was there an effect of the relative conspicuousness or crypsis of the novel prey. Poeciliids were, however, less likely than sticklebacks to drive the novel prey morph to fixation. These results provide strong evidence for the widespread existence of DC in fish populations from a range of habitats, but not all fish species exhibit this trait at the same frequency.

Efficacy of commercially available products against Gyrodactylus turnbulli infections on guppies Poecilia reticulata

The demand for ornamental fish has led to a steep rise in aquaculture for the hobbyist trade, promoting the emergence, persistence and spread of various infectious diseases. Complete control of disease outbreaks with antibiotics and chemical-based medicines is rare, but plant compounds may herald potential alternatives effective against a range of pathogens. Melafix® and Pimafix® are formulated with the essential oils cajuput (Melaleuca cajuputi) and West Indian bay (Pimenta racemosa) and are marketed against bacterial and fungal infections, respectively. Previous experiments showed high efficacy of emulsified cajuput oil against gyrodactylids; the current study tested Melafix® and Pimafix® and their individual compounds against Gyrodactylus turnbulli infecting the guppies Poecilia reticulata. In particular, a combination treatment of Melafix® and Pimafix® was highly effective at reducing in vitro survival of parasites from 15 to 2 h and eradicating 95% of gyrodactylids in vivo. The unexpected high efficacy of this combination treatment is likely explained by the high content of terpenes and phenol propanoids in the cajuput and West Indian bay oils, as well as the anti-helminthic properties of the emulsifier Crovol PK 70. Hence, Melafix® and Pimafix® effectively reduce gyrodactylid burdens on fish, increasing the chances of efficient disease control in ornamental fish.

Haematology and plasma chemistry of the red top ice blue mbuna cichlid (Metriaclima greshakei).

Clinical haematology and blood plasma chemistry can be used as a valuable tool to provide substantial diagnostic information for fish. A wide range of parameters can be used to assess nutritional status, digestive function, disease identification, routine metabolic levels, general physiological status and even the assessment and management of wild fish populations. However to evaluate such data accurately, baseline reference intervals for each measurable parameter must be established for the species of fish in question. Baseline data for ornamental fish species are limited, as research is more commonly conducted using commercially cultured fish. Blood samples were collected from sixteen red top ice blue cichlids (Metriaclima greshakei), an ornamental freshwater fish, to describe a range of haematology and plasma chemistry parameters. Since this cichlid is fairly large in comparison with most tropical ornamental fish, two independent blood samples were taken to assess a large range of parameters. No significant differences were noted between sample periods for any parameter. Values obtained for a large number of parameters were similar to those established for other closely related fish species such as tilapia (Oreochromis spp.). In addition to reporting the first set of blood values for M. Greshakei, to our knowledge, this study highlights the possibility of using previously established data for cultured cichlid species in studies with ornamental cichlid fish.