Dorota Górniak

Culturable bacteria community development in postglacial soils of Ecology Glacier, King George Island, Antarctica

Glacier forelands are excellent sites in which to study microbial succession because conditions change rapidly in the emerging soil. Development of the bacterial community was studied along two transects on lateral moraines of Ecology Glacier, King George Island, by culture-dependent and culture-independent approaches (denaturating gradient gel electrophoresis). Environmental conditions such as cryoturbation and soil composition affected both abundance and phylogenetic diversity of bacterial communities. Microbiocenosis structure along transect 1 (severe cryoturbation) differed markedly from that along transect 2 (minor cryoturbation). Soil physical and chemical factors changed along the chronosequence (time since exposure) and influenced the taxonomic diversity of cultivated bacteria, particularly along transect 2. Arthrobacter spp. played a pioneer role and were present in all soil samples, but were most abundant along transect 1. Cultivated bacteria isolated from transect 2 were taxonomically more diverse than those cultivated from transect 1; those from transect 1 tended to express a broader range of enzyme and assimilation activities. Our data suggest that cryoturbation is a major factor in controlling bacterial community development in postglacial soils, shed light on microbial succession in glacier forelands, and add a new parameter to models that describe succession phenomena.

Evidence of adaptation, niche separation and microevolution within the genus Polaromonas on Arctic and Antarctic glacial surfaces

Polaromonas is one of the most abundant genera found on glacier surfaces, yet its ecology remains poorly described. Investigations made to date point towards a uniform distribution of Polaromonas phylotypes across the globe. We compared 43 Polaromonas isolates obtained from surfaces of Arctic and Antarctic glaciers to address this issue. 16S rRNA gene sequences, intergenic transcribed spacers (ITS) and metabolic fingerprinting showed great differences between hemispheres but also between neighboring glaciers. Phylogenetic distance between Arctic and Antarctic isolates indicated separate species. The Arctic group clustered similarly, when constructing dendrograms based on 16S rRNA gene and ITS sequences, as well as metabolic traits. The Antarctic strains, although almost identical considering 16S rRNA genes, diverged into 2 groups based on the ITS sequences and metabolic traits, suggesting recent niche separation. Certain phenotypic traits pointed towards cell adaptation to specific conditions on a particular glacier, like varying pH levels. Collected data suggest, that seeding of glacial surfaces with Polaromonas cells transported by various means, is of greater efficiency on local than global scales. Selection mechanisms present of glacial surfaces reduce the deposited Polaromonas diversity, causing subsequent adaptation to prevailing environmental conditions. Furthermore, interactions with other supraglacial microbiota, like algae cells may drive postselectional niche separation and microevolution within the Polaromonas genus.

The Diversity of Bacteria Isolated from Antarctic Freshwater Reservoirs Possessing the Ability to Produce Polyhydroxyalkanoates

The diversity of polyhydroxyalkanoates-producing bacteria in freshwater reservoirs in the Ecology Glacier foreland, Antarctica, was examined by a cultivation-dependent method. Isolated strains were analyzed phylogenetically by 16S rRNA gene sequencing, and classified as members of Alpha-, Beta-, or Gammaproteobacteria classes. Polymerase chain reaction was used to detect PHA synthase genes. Potential polyhydroxyalkanoates (PHAs) producers belonging mainly to Pseudomonas sp., and Janthinobacterium sp. were isolated from all five sampling sites, suggesting that PHA synthesis is a common bacterial feature at pioneer sites. All Pseudomonas strains had the genetic potential to synthesize medium-chain-length PHAs, whereas some isolated Janthinobacterium strains might produce short-chain-length PHAs or medium-chain-length PHAs. It is the first report revealing that Janthinobacterium species could have the potential to produce medium-chain-length PHAs.

Morphological and Ultrastructural Changes of Organelles in Leaf Mesophyll Cells of the Arctic and Antarctic Plants of Poaceae Family Under Cold Influence

Abstract The mesophyll cells of four species of Poaceae flowering plants growing in polar regions were studied—Deschampsia antarctica Desv. from the region of the Admiralty Bay on King George Island (West Antarctica) and D. alpina (L.) Roem. Sch., Poa alpina L. var. vivipara and P. arctica R. Br. var. vivipara from the Hornsund region of Spitsbergen island (Arctic). Ultrastructural changes were analyzed in the organelles of plants growing in Arctic and Antarctic habitats and plants grown in greenhouse, including plants exposed to short-term cold stress. The cell organelles were characterized by structural dynamics. Their morphological plasticity was manifested by elongation, formation of protrusions in the direction of adjacent organelles, as well as cytoplasm-filled pockets and invaginations that increase the contact area and reduce the distance between cell compartments. D. antarctica and P. alpina var. vivipara plants were characterized by highly dynamic cell nuclei with invaginations of the nuclear membrane filled with cytoplasm and organelles, high morphological plasticity, and conformational dynamics of chloroplasts and mitochondria, manifested by variations in the electron-optical density of matrix, membranes, and envelopes. The above could suggest that the studied taxa and their metabolic mechanisms had adapted to severe climates and changing conditions of the polar regions.

Microbial community changes along the Ecology Glacier ablation zone (King George Island, Antarctica)

In recent years, glacial surfaces have received much attention as microbial habitats of diverse photoautotrophic and heterotrophic cells. Supraglacial ecosystems are annually covered and uncovered by snow. The aim of this study is to investigate the microbial community response to changing environmental conditions in a transect following the receding snow line on the surface of Ecology Glacier (King George Island, Antarctica). Parameters of surface ice and cryoconite holes included chemical composition of ice and sediment, Bacteria diversity by denaturating gradient gel electrophoresis, microbial functional diversity (Biolog Ecoplates), and microbial counts (epifluorescence microscopy, colony-forming units). Data demonstrated profound differences between surface ice and cryoconite holes. Changing environmental factors along the transect influenced composition and abundance of the microbiocenosis in both habitat types. Several parameters correlated positively with distance from the glacier edge, including the cell morphotype Shannon index, chlorophyll a, nitrogen, and seston concentrations. Suspended solid content positively correlated with microbial abundance and diversity. Nitrogen and phosphorus were limiting factors of microbial growth as amounts of organic nitrogen and phosphorus positively correlated with the cell numbers, fission rates, and photoautotroph contribution. Our findings indicate that microbial community shows a response in terms of abundance and diversity to exposure of the glacial surface as snow-cover melts. To our knowledge, this is the first study to recognize a microbial development pattern on a glacier surface in connection with the receding snow line. This may help better understand variability within supraglacial habitats, correct sampling procedures, and inform biocenotic development models.

Hydrochemical properties, bacterioplankton abundance and biomass in the meromictic Lake Starodworskie in 2004

Hydrochemical properties, bacterioplankton abundance and biomass in the meromictic Lake Starodworskie in 2004 Physicochemical and microbiological examinations were conducted on the meromictic Lake Starodworskie located in Olsztyn. The temperature profile revealed a characteristic, small increase in temperature at a depth between 13 and 14 m, and permanent anaerobic conditions in the deeper water layers. Accumulation of dissolved mineral substances enhanced the thermal stability of the lake waters; electrolytic conductivity values in the water deeper than 15 m oscillated within the range of 533 - 568 μS cm-1.

Identification of miniature plasmids in psychrophilic Arctic bacteria of the genus Variovorax

The Svalbard archipelago (Spitsbergen Island) is the northernmost landmass in the European Arctic and has a variety of small- and medium-sized glaciers. The plasmidome of eleven psychrophilic strains of Variovorax spp. isolated from the ice surface of Hans and Werenskiold Glaciers of Spitsbergen Island, was defined. This analysis revealed the presence of six plasmids whose nucleotide sequences have been determined. Four of them, exhibiting high reciprocal sequence similarity, possess unique structures, since their genomes lack any recognized genes. These miniature replicons, not exceeding 1 kb in size, include pHW69V1 (746 bp), which is the smallest autonomous replicon so far identified in free-living bacteria. The miniature plasmids share no similarity with known sequences present in the databases. In silico and experimental analyses identified conserved DNA regions essential for the initiation of replication of these replicons.

Phytoplankton dominance structure and abundance as indicators of the trophic state and ecological status of Lake Kortowskie (northeast Poland) restored with selective hypolimnetic withdrawal

Abstract The objective of the study was to identify seasonal changes in phytoplankton taxonomic structure and development rates to characterize phytoplankton communities as determinants of the trophic and ecological conditions of Lake Kortowskie in northeast Poland which has been undergoing restoration with selective hypolimnetic withdrawal. The lake was designated as being of high trophic state and bad ecological status based on intense phytoplankton growth, the strong dominance and persistent occurrence of blue-green algae, and seasonal modifications in phytoplankton taxonomic structure and assemblage growth rates. The ecological status of the lake assessed in 2011 corresponds to that determined in 1987-1990 and 1999 and indicates that eutrophication in Lake Kortowskie is progressing.