Dorothea Leonhäuser
RWTH Aachen University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Dorothea Leonhäuser.
Journal of Biomaterials Applications | 2016
Dorothea Leonhäuser; Michael Vogt; Rene Tolba; Joachim Grosse
The aging society has a deep impact on patient care in urology. The number of patients in need of partial or whole bladder wall replacement is increasing simultaneously with the number of cancer incidents. Therefore, urological research requires a model of bladder wall replacement in adult and elderly people. Two types of porcine collagen I/III scaffolds were used in vitro for comparison of cell growth of two different pig breeds at different growth stages. Scaffolds were characterised with scanning electron and laser scanning microscopy. Urothelial and detrusor smooth muscle cells were isolated from 15 adult Göttingen minipigs and 15 juvenile German Landrace pigs. Growth behaviour was examined in cell culture and seeded onto the collagen scaffolds via immunohistochemistry, two-photon laser scanning microscopy and a viability assay. The collagen scaffolds showed different structured surfaces which are appropriate for seeding of the two different cell types. Moisturisation of the scaffolds resulted in a change of the structure. Cell growth of German Landrace urothelial cells and smooth muscle cells was significantly higher than cell growth of the Göttingen minipig cells. Seeding of scaffolds with both cell types from both pig races was possible which could be shown by immunohistochemistry and two-photon laser scanning microscopy. Growth behaviour on the scaffolds was significantly increased for the German Landrace compared to Göttingen minipig. Nevertheless, seeding with the adult Göttingen minipig cells resulted in a closed layer on the surface and urothelial cells and smooth muscle cells showed increasing growth until day 14. The results show that these collagen scaffolds are adequate for the seeding with vesical cells. Moreover, they seem appropriate for the use as an in vitro model for the adult or elderly as the cells of the adult Göttingen minipig too, show good growth behaviour.
Journal of Translational Medicine | 2017
Dorothea Leonhäuser; Katja Stollenwerk; Volker Seifarth; Isabella Zraik; Michael Vogt; Pramod Kadaba Srinivasan; Rene Tolba; Joachim Grosse
Archive | 2014
Thomas Schlebusch; Joachim Grosse; Steffen Nienke; Dorothea Leonhäuser; Steffen Leonhardt
Biomedical Engineering Online | 2018
Dorothea Leonhäuser; Carlos Castelar; Thomas Schlebusch; Martin Rohm; Rüdiger Rupp; Steffen Leonhardt; Marian Walter; Joachim Grosse
Archive | 2017
Dorothea Leonhäuser; U. Klinge; Rene Tolba
ics.org | 2016
Dorothea Leonhäuser; Jasmin Kranz; Regina Leidolf; Ulrich Schwantes; Joachim Geyer; Joachim Grosse
Neurourology and Urodynamics | 2016
Carlos Castelar; Dorothea Leonhäuser; Martin Rohm; Rüdiger Rupp; Isabella Zraik; Steffen Leonhardt; Marian Walter; Joachim Grosse
ics.org | 2015
Stephanie Schmidt; Jasmin Kranz; Regina Leidolf; Dorothea Leonhäuser; Joachim Grosse; Joachim Geyer
ics.org | 2014
Dorothea Leonhäuser; Thomas Schlebusch; Isabella Zraik; Steffen Leonhardt; Joachim Grosse
Neurourology and Urodynamics | 2014
Nadine Huppertz; Carmen Fera; Dorothea Leonhäuser; Katja Strick; Isabella Zraik; Ulrich Schwantes; Rene Tolba; Joachim Grosse