Douglas A. Fisher
Pfizer
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Featured researches published by Douglas A. Fisher.
Acta Crystallographica Section D-biological Crystallography | 2009
Artem G. Evdokimov; Igor Voznesensky; Kimberly F. Fennell; Marie Anderson; James F. Smith; Douglas A. Fisher
Bacterial persistence is the ability of individual cells to randomly enter a period of dormancy during which the cells are protected against antibiotics. In Escherichia coli, persistence is regulated by the activity of a protein kinase HipA and its DNA-binding partner HipB, which is a strong inhibitor of both HipA activity and hip operon transcription. The crystal structure of the HipBA complex was solved by application of the SAD technique to a mercury derivative. In this article, the fortuitous and interesting effect of mercury soaks on the native HipBA crystals is discussed as well as the intriguing tryptophan-binding pocket found on the HipA surface. A HipA-regulation model is also proposed that is consistent with the available structural and biochemical data.
Journal of Biomolecular Screening | 2007
Brian J. Stockman; Ian J. Lodovice; Douglas A. Fisher; Alexander S. McColl; Zhi Xie
Nicotinamide adenine dinucleotide synthetase (NadE) is an essential enzyme for bacterial pathogens and is thus a promising antibacterial target. It catalyzes the conversion of nicotinic acid adenine dinucleotide to nicotinamide adenine dinucleotide. Changes in chemical shifts that occur in the nicotinic acid ring as it is converted to nicotinamide can be used for monitoring the reaction. A robust nuclear magnetic resonance—based activity assay was developed using robotically controlled reaction initiation and quenching. The single-enzyme assay has less potential for false positives compared to a coupled activity assay and is especially well suited to the high concentration of compounds in fragment screens. The assay has been used to screen fragment libraries for NadE inhibitors. ( Journal of Biomolecular Screening 2007:457-463)
Science | 2003
Paul S. Changelian; Mark Edward Flanagan; Douglas J. Ball; Craig R. Kent; Kelly S. Magnuson; William H. Martin; Bonnie J. Rizzuti; Perry S. Sawyer; Bret D. Perry; Sandra P. McCurdy; Elizabeth M. Kudlacz; Maryrose J. Conklyn; Eileen A. Elliott; Erika R. Koslov; Michael B. Fisher; Timothy J. Strelevitz; Kwansik Yoon; David A. Whipple; Jianmin Sun; Michael John Munchhof; John L. Doty; Jeffrey M. Casavant; Todd Andrew Blumenkopf; Michael Hines; Matthew Frank Brown; Brett M. Lillie; Chakrapani Subramanyam; Chang Shang-Poa; Anthony J. Milici; Gretchen E Beckius
Journal of Biological Chemistry | 1998
Douglas A. Fisher; James F. Smith; J.S. Pillar; Suzanne H. St. Denis; John B. Cheng
Biochemical and Biophysical Research Communications | 1998
Douglas A. Fisher; James F. Smith; J.S. Pillar; Suzanne H. St. Denis; John B. Cheng
Journal of Immunology | 1997
Binfeng Lu; Martin Reichel; Douglas A. Fisher; James F. Smith; Paul Rothman
Archive | 2002
Douglas A. Fisher; Michael D. Robbins
Archive | 1995
Douglas A. Fisher
Bioorganic & Medicinal Chemistry Letters | 2006
Robert J. Chambers; Kristin Abrams; Tessa A. Castleberry; John B. Cheng; Douglas A. Fisher; Ajith V. Kamath; Anthony Marfat; David O. Nettleton; Joann D. Pillar; Eben D. Salter; Alissa L. Sheils; John T. Shirley; Claudia R. Turner; John P. Umland; Kelvin Lam
Archive | 1995
Douglas A. Fisher; Michael D. Robbins