Drew Sullivan

Hormonal regulation of ovalbumin synthesis in the chick oviduct.

Estrogens and progesterone regulate the differentiation and function of chick oviduct (1–4). Our attention has been focused on hormonal regulation of ovalbumin synthesis, since this single polypeptide comprises 50–60% of the protein synthesized in the fully differentiated oviduct. This feature allows potentially for isolation of the molecular elements involved in specific protein synthesis, including specific polysomes, mRNA, and genes, and an analysis of various regulatory steps between transcription and translation of specific mRNAs, as affected by developmental and hormonal variables.

[59] Immunoadsorption of ovalbumin synthesizing polysomes and partial purification of ovalbumin messenger RNA

Publisher Summary An understanding of the mechanisms underlying the regulation of specific protein synthesis requires the ability to isolate and quantitate the various elements that may be rate limiting in specific protein synthesis, including specific mRNA. In special instances a specific mRNA may be isolated because it constitutes such a large portion of the total mRNA of a cell type, and is of a unique size such that it can be separated from other RNA species by size; e.g., globin mRNA in red blood cells, or by virtue of unique physical properties resulting from an unusual base composition, e.g., silk fibroin mRNA. However the majority of mRNAs will require other methods for isolation.

Erythrocyte Enzyme Disorders in Children

Erythrocyte metabolic abnormalities should be considered as a possible cause of hemolysis when there is no evidence of an immune-mediated hemolytic anemia, no consumptive red blood cell disorder, no morophologic or laboratory data to suggest a problem of the red cell membrane, and no evidence of a quantitative or qualitative defect in hemoglobin synthesis. Glucose-6-phosphate dehydrogenase deficiency is clearly the most common enzyme deficiency causing clinical problems.