Duan Chuanren

Low ultrasonic stimulates fermentation of riboflavin producing strain Ecemothecium ashbyii

The effect of low ultrasonic stimulation on fermentation of riboflavin producing strain Ecemothecium ashbyii was investigated. In this paper, several key biochemical parameters related to cell growth were measured. The results of the present study show that all of the given different frequency of low ultrasonic treatment could promote mycelium growth, at the same time also improve output of riboflavin, and the optimum frequency was about 24 kHz. After 24 kHz ultrasonic incontinuous treatments, the fermentation time was shortened by 36 h and productivity rate of riboflavin was increased by about 5 times compared with the control groups.

Effects of sound field on the growth of Chrysanthemum callus

The effects of sound field on Chrysanthemum callus were studied. The field of alternative stress was generated through a strong sound field system set up in our lab, and the activity of SOD, the content of soluble proteins, the activity of IAA oxidase and the absorption rate of calcium were measured. We found that the sound field stimulation has dual effects, which can enhance or inhibit the growth of Chrysanthemum callus, the growth effects of sound field on Chrysanthemum callus depended greatly on the intensity and frequency of sound field. The activity of SOD, the content of soluble proteins and the absorption rate of calcium in callus increased with the intensity and frequency increasing. However, those indexes began to decrease when the intensity and frequency went beyond the limit of 100 dB and 800 Hz, respectively. However, the changing tendency of IAA oxidase activity was reversed to the above three indexes. We draw a conclusion that the optimal stimulation conditions are 100 dB and 800 Hz. Under the conditions, the sound field can distinctly enhance the growth of Chrysanthemum callus. We think that moderate stress stimulation can enhance the assimilation of tissues or cells, improve their physiological activity and accelerate the growth of plants, this moderate stress stimulation is helpful to the growth of plant tissues.

The influence of low-intensity ultrasonic on some physiological characteristics of Saccharomyces cerevisiae

In this paper, we studied about some vital physiological characteristics of Saccharomyces cerevisiae at logarithmic phase under ultrasonic stimulation including the stimulated conditions of the 24 kHz in frequency, 2 W in the power efficiency, 1 s for every 15 s in the stimulation time and 30 min in the duration cycle. After S. cerevisiae was inoculated and stimulated for passages, the effects of low ultrasound on the fermentation strength and proteinase activity of S. cerevisiae have been studied. Furthermore, cell flocculation and the ratio of ascospore production were detected in descendant S. cerevisiae. The results showed that ultrasonic stimulation specialized as above could enhanced the fermentation strength and proteinase activity of parents, but it had no influence on the fermentation strength and the ratio of ascospore production of the descendants while reduced the flocculation of the descendants. So, it can be concluded that appropriate low-intensity ultrasound will not affect the primary physiological characteristics of S. cerevisiae.

Effect of sound wave on the metabolism of chrysanthemum roots

Environmental factors can greatly influence the growth of plants. In this paper, the effect of sound stimulation on the metabolism of chrysanthemum roots was studied and it was found that the growth of roots was not inhibited but accelerated under suitable sound stimulation. And the content of soluble sugar and protein and the activity of amylase all increased significantly, which indicated that sound stimulation could enhance the metabolism of roots and the growth of chrysanthemum.

The research into the influence of low-intensity ultrasonic on the growth of S.cerevisiaes

In this paper, an orthogonal experiment was designed which was involved in four levels and five factors including the ultrasonic frequency and power efficiency, the interval between two stimulations, the each stimulation time, and the total ultrasonic stimulation duration. The influences of ultrasonic on the growth of Saccharomyces cerevisiaes were studied when S. cerevisiaes were in lag phase, exponential phase, and stationary phase, respectively. Meanwhile, the optimum parameters of ultrasonic stimulation had been obtained. Based on the experimental data, it was found that the low-intensity ultrasonic could accelerate the growth of S. cerevisiaes in the lag phase and the exponential phase under some appropriate conditions, but almost had no influence on the growth of S. cerevisiaes in the stationary phase. And the optimum parameters of ultrasonic stimulating S. cerevisiaes in lag phase were different from that in exponential phase. Furthermore, even if both S. cerevisiaes in the lag phase and in the exponential phase were under the conditions of their own optimum parameters, the rate of S. cerevisiaes growth was different, too. The stimulation of low-intensity ultrasonic had little influences on the temperature of the medium in a way, but it could somewhat raise the mediums pH and bring about foam when S. cerevisiaes were in the exponential phase.

Effects of sound stimulation on protective enzyme activities and peroxidase isoenzymes of chrysanthemum

Abstract One of the important fields in cell biology is physical stimulus on the growth of plant cells and organs. The effects of strong sound wave on thermodynamic properties, cell cycles and calcium ion signaling transduction of plants have been studied. In this work, chrysanthemum seedlings are treated by sound wave with a certain intensity (100 db) and frequency (1000 Hz) for 3, 6, 9, 12 and 15 days, respectively, and each day for 60 min. The research results show that the activities of protective enzymes and the peroxidase isoenzymes increase at varying degrees. Compared with the control group, there is no new enzyme band in POD electrophoretogram for experimental groups. Our experimental data imply that better promotion for chrysanthemum is obtained under sound wave for 9 days.

Effect of sound wave on the synthesis of nucleic acid and protein in chrysanthemum

Abstract Environmental factors can greatly influence the growth and development of plants, even the genetic character. It had been found that sound stimulation had an obvious effect on the growth and development of plants. But the mechanism of how sound affected the growth of plants is not clear so far. In this paper, we studied the effect of sound on the nucleic acid and soluble protein to explore the mechanism of the biological effect of sound. It was found that sound wave had no obvious influence on the content of DNA but accelerated the synthesis of RNA and soluble protein. By means of the assay of relation, the content of soluble protein had a very close relationship with that of RNA. This result indicated that some stress-induced genes might be switched on under sound stimulation and the level of transcription increased.

Effect of sound stimulation on roots growth and plasmalemma H+-ATPase activity of chrysanthemum (Gerbera jamesonii)

Abstract Chrysanthemum seedlings are treated by sound wave with a certain intensity (100 db) and frequency (1000 Hz) for 3, 6, 9, 12 and 15 days, respectively, and each day for 60 min. The results show that the activity of roots and the content of soluble protein increase greatly under sound stimulation. The activity of plasmalemma H + -ATPase increases while stimulated by sound wave. The concentration of Ca 2+ , the Ca 2+ passage blocker (Verapamil) and the Ca 2+ carrier (A 23187 ) can affect the activity of plasmalemma H + -ATPase and the protein kinase inhibitor (Staurosporine) can decrease the activity. The results indicate that the phosphorylation–dephosphorylation process probably regulates the activity of plasmalemma H + -ATPase under sound stimulation.

Effects of sound stimulation on energy metabolism of Actinidia chinensis callus

More and more attentions have been taken to the effects of environmental stresses on the growth and development of plant cells and tissues. Making efforts on this field would enhance plant adaptability to varied environments and implement extensively efficient agricultural technologies. Using callus cultured from stalks of Actinidia chinensis, this study dealt with the effects of sound stimulation on plant cell energy metabolism, i.e., content of ATP. The results indicated that there occurs bi-directional effect of ATP content on sound field stimulation. The sound intensity of about 100 dB and sound frequency of approximately 1000 Hz are optimal external stresses for energy metabolism of A. chinensis. The experimental data showed that moderate sound field would be advantageous to growth and development of woody plants. The mechanisms of ATP content effects of sound stimulation on A. chinensis callus were comprehensively discussed in the light of cytobiology and molecular biology. Whereas the essential mechanisms of biological effects of environmental stresses remain further research.

RETRACTED: Effect of sound stimulation on cell cycle of chrysanthemum (Gerbera jamesonii)

Plant growth can be considered as the sum of cell proliferation in the meristems and the subsequent elongation of cells. The continuous proliferative capacity of plant cells is crucial for the production of new organs and thus has a significant impact on plant architecture. Now it had been found that the relationship between environmental factors and growth of plant was very close. And in this paper, the effect of sound stimulation on the cell cycle of chrysanthemum was studied to further explore the mechanism of biological effect of sound stimulation. # 2002 Elsevier Science B.V. All rights reserved.