Dwight Werren

DNA strand breaks, oxidative damage, and 1-OH pyrene in roofers with coal-tar pitch dust and/or asphalt fume exposure

OBJECTIVE To determine the potential for asphalt fume exposure to increase DNA damage, we conducted a cross-sectional study of roofers involved in the application of roofing asphalt. METHODS DNA strand breaks and the ratio of 8-hydroxydeoxyguanosine (8-OHdG) to 2-deoxyguanosine (dG) were measured in peripheral blood leukocytes of roofers. In addition, urinary excretion of 8-OHdG and 8-epi-prostaglandin F2alpha (8-epi-PGF) was also measured. The study population consisted of 26 roofers exposed to roofing asphalt and 15 construction workers not exposed to asphalt during the past 5 years. A subset of asphalt roofers (n = 19) was exposed to coal-tar pitch dust (coal tar) during removal of existing roofs prior to applying hot asphalt. Personal air monitoring was performed for one work-week to measure exposure to total particulates, benzene-soluble fraction of total particulates, and polycyclic aromatic compounds (PACs). Urinary 1-OH-pyrene levels were measured as an internal biomarker of PAC exposure. RESULTS Full-shift breathing zone measurements for total particulates, benzene-solubles and PACs were significantly higher for coal-tar exposed workers than for roofers not exposed to coal tar. Similarly, urinary 1-OH-pyrene levels were higher in coal-tar exposed roofers than roofers not exposed to coal tar. Total particulates or benzene-soluble fractions were not associated with urinary 1-OH-pyrene, but PAC exposure was highly correlated with urinary 1-OH-pyrene. When stratified by 1-OH-pyrene excretion, DNA strand breaks increased in a dose-dependent manner, and leukocyte 8-OHdG/dG decreased in a dose-dependent manner. Significant changes in DNA damage appeared to be linked to PACs from coal-tar exposure, although asphalt fume alone was associated with a small but significant increase in urinary 1-OH-pyrene and DNA strand breaks. CONCLUSIONS Results are consistent with previous reports that asphalt or coal-tar exposure can cause DNA damage. Urinary 8-epi-PGF remained relatively constant during the week for virtually all subjects, regardless of exposure indicating that neither asphalt nor coal-tar exposure induces an overt oxidative stress. A small, but statistically significant increase in 8OHdG was evident in end-of-week urine samples compared with start-of-week urine samples in roofers exposed to coal-tar. The increase in urinary 8OHdG coupled with the decrease in leukocyte 8-OHdG/dG, suggests that coal-tar exposure induces protective or repair mechanisms that result in reduced levels of steady-state oxidative-DNA damage.

Oxidative stress and DNA damage in Fischer rats following acute exposure to trichloroethylene or perchloroethylene.

Oxidative DNA damage is emerging as an biomarker of effect in studies assessing the health risks of occupational chemicals. Trichloroethylene (TCE) and perchloroethylene (PERC) are used in the dry cleaning industry and their metabolism can produce reactive oxygen compounds. The present study examined the potential for TCE and PERC to induce oxidative DNA damage in rats that was detectable as increased urinary excretion of 8-hydroxydeoxyguanosine (8OHdG). Thiobarbaturic acid reactive substances (TBARS) and 8-epiprostaglandin F2alpha (8epiPGF) were also measured as biomarkers of increased oxidative stress. Male Fischer rats were administered a single i.p. injection of 0, 100, 500, or 1000 mg/kg of PERC or TCE. Control rats received only vehicle (1:4 v/v of Alkamuls/water). A positive control group received 100 mg/kg 2-nitropropane (2NP). Rats were sacrificed 24 h after dosing. In rats receiving 2NP or TCE but not PERC, TBARS and the 8OHdG/dG ratios were significantly elevated in liver. Lymphocyte 8OHdG/dG was not affected significantly by 2NP, TCE or PERC. In rats receiving 2NP, urinary excretion of 8OHdG and 8epiPGF2 were significantly increased. In rats receiving TCE or PERC, significant increases in 8epiPGF2 or 8OHdG were not evident. Results indicate that a single high dose of TCE, but not PERC, can induce an increase in oxidative DNA damage in rat liver. However, the usefulness of 8OHdG as a biomarker of TCE-induced oxidative DNA damage is questionable.

Efficacy of the Revised NIOSH Lifting Equation to Predict Risk of Low Back Pain Due to Manual Lifting Expanded Cross-Sectional Analysis

Objective: To evaluate whether the Revised NIOSH Lifting Equation (RNLE) is a valid tool for assessing risk of low back pain (LBP) due to manual lifting by using combined data from two cross-sectional studies of 1-year prevalence. Methods: Results from a symptom and occupational history questionnaire and RNLE analysis for 677 subjects employed in 125 manual lifting jobs at nine industrial sites were combined from two studies. Results: The odds of LBP increased as the lifting index (LI) increased from 1.0 to 3.0. A statistically significant odds ratio (OR) was found for both the 1 < LI ⩽ 2 (OR = 1.81) and the 2 < LI ⩽ 3 categories (OR = 2.26). For jobs with an LI value greater than 3.0, however, the OR remained nonsignificant. The 2 < LI ⩽ 3 group remained statistically significant after adjusting for age, gender, body mass index, and psychosocial factors. Conclusions: It is clear that as the LI increases, the risk of LBP increases. Longitudinal studies are needed.

Efficacy of the Revised NIOSH Lifting Equation to Predict Risk of Low-Back Pain Associated With Manual Lifting: A One-Year Prospective Study

Objective: The objective was to evaluate the efficacy of the Revised National Institute for Occupational Safety and Health (NIOSH) lifting equation (RNLE) to predict risk of low-back pain (LBP). Background: In 1993, NIOSH published the RNLE as a risk assessment method for LBP associated with manual lifting. To date, there has been little research evaluating the RNLE as a predictor of the risk of LBP using a prospective design. Methods: A total of 78 healthy industrial workers’ baseline LBP risk exposures and self-reported LBP at one-year follow-up were investigated. The composite lifting index (CLI), the outcome measure of the RNLE for analyzing multiple lifting tasks, was used as the main risk predictor. The risk was estimated using the mean and maximum CLI variables at baseline and self-reported LBP during the follow-up. Odds ratios (ORs) were calculated using a logistic regression analysis adjusted for covariates that included personal factors, physical activities outside of work, job factors, and work-related psychosocial characteristics. Results: The one-year self-reported LBP incidence was 32.1%. After controlling for history of prior LBP, supervisory support, and job strain, the categorical mean and maximum CLI above 2 had a significant relationship (OR = 5.1–6.5) with self-reported LBP, as compared with the CLI below or equal to 1. The correlation between the continuous CLI variables and LBP was unclear. Conclusions: The CLI > 2 threshold may be useful for predicting self-reported LBP. Research with a larger sample size is needed to clarify the exposure–response relationship between the CLI and LBP.

Human posture simulation to assess cumulative spinal load due to manual lifting. Part I: methods

The estimation of cumulative spinal load (CSL) resulting from exposure to manual materials handling (MMH) may provide a sensitive method for assessing the risk of highly varying exposures. This article reports on a CSL method that involves human posture simulation of workers from videotape in order to assess spinal load exposures due to MMH. The proposed method appears to be sensitive to different durations of exposure, easy to use and useful for assessing jobs with a high degree of variability in task characteristics between lifts. Although the method remains to be validated, it appears to be a useful addition to the range of tools available for assessing manual lifting exposures in worksite-based epidemiologic studies. Ergonomic methods are lacking for assessing highly variable MMH tasks, such as tasks found in warehousing. The existing methods do not include sufficient factors to account for variable exposure patterns or tasks with highly variable task characteristics, such as varying load weights and lift geometries. The CSL assessment method described in this article may provide a way to evaluate these types of tasks in order to assess the overall risk of workers developing work-related musculoskeletal disorders.

Human posture simulation to assess cumulative spinal load due to manual lifting. Part II: accuracy and precision

For assessing a large number of variable manual lifting jobs, posture specification for using the University of Michigan Three Dimensional Static Strength Prediction Program and the revised National Institute for Occupational Safety and Health Lifting Equation may be time-consuming, tedious and subject to human errors. To expedite data analysis with desirable accuracy and precision for the two risk assessment tools, a new data analysis method based on human posture simulation was developed and evaluated. The accuracy and precision of the posture simulation method were evaluated by a repeated measures study design with six postures, three viewing angles and three trial repetitions as experimental factors. The effects of the experimental factors on the average accuracy and precision of the simulation method are reported and discussed. The study results also demonstrated pros and cons of human posture simulation as a means of posture specification for ergonomic risk assessments. The findings about the accuracy and precision of the human posture simulation method for quantifying the risk of musculoskeletal disorders due to manual materials handling may provide researchers with a new way of ergonomic assessments.

The Effectiveness of a Shield in Reducing Operator Exposure to Radiofrequency Radiation from a Dielectric Heater

Abstract The objective of this study was to design and install a shield on a radiofrequency (RF) dielectric heater used in the water bed industry and to determine its effectiveness in reducing worker exposures. In work sites where industrial dielectric heating is utilized, occupational RF radiation exposures frequently exceed occupational exposure limits. A water bed manufacturer that used dielectric heaters in its production operations agreed to participate in this developmental study. The mean-squared (ms) RF electric (E 2) and magnetic (H 2) field strengths, the root-mean-squared (rms) RF-induced foot current and the heater frequency were measured for each unit. A heater utilizing a common sealing process and producing high worker exposures was selected for this study. The water bed mattress is a large, bulky product and can not be contained inside the shield. Thus, the shield required a slot or opening to allow passage of the material between the applicator plates for sealing while minimizing the leak...