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Dive into the research topics where E.D. Olthof is active.

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Featured researches published by E.D. Olthof.


PLOS ONE | 2014

Taurolidine lock is superior to heparin lock in the prevention of catheter related bloodstream infections and occlusions.

E.D. Olthof; Michelle W. Versleijen; Getty Huisman-de Waal; Ton Feuth; Wietske Kievit; Geert Wanten

Background and Aims Patients on home parenteral nutrition (HPN) are at risk for catheter-related complications; mainly infections and occlusions. We have previously shown in HPN patients presenting with catheter sepsis that catheter locking with taurolidine dramatically reduced re-infections when compared with heparin. Our HPN population therefore switched from heparin to taurolidine in 2008. The aim of the present study was to compare long-term effects of this catheter lock strategy on the occurrence of catheter-related bloodstream infections and occlusions in HPN patients. Methods Data of catheter-related complications were retrospectively collected from 212 patients who received HPN between January 2000 and November 2011, comprising 545 and 200 catheters during catheter lock therapy with heparin and taurolidine, respectively. We evaluated catheter-related bloodstream infection and occlusion incidence rates using Poisson-normal regression analysis. Incidence rate ratios were calculated by dividing incidence rates of heparin by those of taurolidine, adjusting for underlying disease, use of anticoagulants or immune suppressives, frequency of HPN/fluid administration, composition of infusion fluids, and duration of HPN/fluid use before catheter creation. Results Bloodstream infection incidence rates were 1.1/year for heparin and 0.2/year for taurolidine locked catheters. Occlusion incidence rates were 0.2/year for heparin and 0.1/year for taurolidine locked catheters. Adjusted incidence ratios of heparin compared to taurolidine were 5.9 (95% confidence interval, 3.9–8.7) for bloodstream infections and 1.9 (95% confidence interval, 1.1–3.1) for occlusions. Conclusions Given that no other procedural changes than the catheter lock strategy were implemented during the observation period, these data strongly suggest that taurolidine decreases catheter-related bloodstream infections and occlusions in HPN patients compared with heparin.


Clinical Nutrition | 2015

Microbiocidal effects of various taurolidine containing catheter lock solutions

E.D. Olthof; R. Nijland; A.F. Gülich; Geert Wanten

BACKGROUND & AIMS We have recently shown that a catheter lock solution containing taurolidine dramatically decreases catheter-related bloodstream infections (CRBSI) in patients on home parenteral nutrition (HPN) when compared to heparin. Since several taurolidine formulations are commercially available, some of which also contain citrate or heparin, we were interested in the effect of these different locks on growth and biofilm formation of fungal, Gram-negative and Gram-positive pathogens that are known to impede HPN treatment. METHODS Clinical isolates obtained during CRBSI of HPN patients were grown in the presence of catheter locks (2% taurolidine, 1.34% taurolidine-citrate, 1.34% taurolidine-citrate-heparin, citrate and heparin) or phosphate buffered saline diluted in lysogeny broth medium for bacteria and sabouraud liquid medium for yeasts. Biofilm formation, assessed by crystal violet staining, and growth of clinical isolates were determined by optical density measurements. RESULTS We found that 12.5× diluted solutions of all taurolidine containing formulations completely prevented growth of Escherichia coli, Staphylococcus aureus and Candida glabrata. Growth of these microbes was detected earlier in 1.34% taurolidine-citrate(-heparin) than in 2% taurolidine, while citrate and heparin did not inhibit growth of clinical isolates compared to PBS. No differences in biofilm formation were found between taurolidine containing solutions. CONCLUSION Taurolidine containing lock solutions prevent growth of fungal, Gram-negative and Gram-positive pathogens. While 2% taurolidine appears to be the most potent in this respect in this in vitro setting, the relevance of the small differences in growth inhibition between the commercially available taurolidine containing lock solutions for clinical practice remains to be established.


Journal of Parenteral and Enteral Nutrition | 2016

No Clinical or Biochemical Evidence for Essential Fatty Acid Deficiency in Home Patients Who Depend on Long-Term Mixed Olive Oil– and Soybean Oil–Based Parenteral Nutrition

E.D. Olthof; Hennie M.J. Roelofs; Helena L. Fisk; Philip C. Calder; Geert Wanten

BACKGROUND Home parenteral nutrition (HPN) patients depend on lipid emulsions as part of their parenteral nutrition regimen to provide essential fatty acids (EFAs). Mixed-oil sources are used in modern lipid emulsions to decrease the amount of proinflammatory EFAs, mainly linoleic acid, which is present in large amounts in soybean oil. It is unknown whether patients who fully depend on such mixed lipids have adequate EFA supply. We therefore evaluated whether HPN patients who depend on mixed olive oil- and soybean oil-based HPN show clinical or biochemical evidence of EFA deficiency. MATERIALS AND METHODS Fatty acid status was assessed in plasma phosphatidylcholine (PC) and peripheral blood mononuclear cells from 30 patients receiving mixed olive oil- and soybean oil-based HPN (>3 months, ≥5 times per week) and 30 healthy controls. Innate immune cell functions were evaluated by assessing expression of surface membrane molecules, and reactive oxygen species, and cytokine production. RESULTS None of the patients or controls showed clinical evidence (skin rash) or biochemical evidence (increased Holman index [>0.2]) for EFA deficiency. The Holman index in plasma PC (median [25th-75th percentile]) was significantly higher in patients (0.019 [0.015-0.028]) compared with controls (0.015 [0.011-0.017]). No differences were found in innate immune cell functions between groups, except for a 3.6-fold higher tumor necrosis factor-α production in patients. CONCLUSION We found no clinical or biochemical evidence that HPN patients who fully and long-term depend on mixed olive oil- and soybean oil-based lipids have an increased risk for EFA deficiency.


Clinical Nutrition Supplements | 2012

PP029-MON A 55% REDUCTION IN HOSPITAL ADMISSION RATE OF PATIENTS ON HOME PARENTERAL NUTRITION AFTER INTRODUCTION OF TAUROLIDINE CATHETER LOCKING: A FOLLOW-UP OF NEARLY 200,000 DAYS

E.D. Olthof; G. Huisman-de Waal; R. Vissers; Geert Wanten

icantly increased in the CT group (CT: 18.0±4.4/b-actin, V: 6.8±0.40/b-actin, P< 0.05). Moreover, the addition of cystine but not theanine significantly increased LPS-induced IL-18 production in THP-1 cells compared with the control (cystine: 461.8±29.9 pg/ml, control: 101.2±8.1 pg/ml, P< 0.05). Conclusion: Our results suggest that CT administration enhances LPS-induced sNK cell activity at least partly through increasing IL-18 production by cystine in monocytes, and that CT may be effective in preventing immunosuppression in patients after surgery.


Toxicology in Vitro | 2015

Immune activation by medium-chain triglyceride-containing lipid emulsions is not modulated by n-3 lipids or toll-like receptor 4

E.D. Olthof; Alexandra F. Gülich; Mike F. Renne; Sija Landman; Leo A. B. Joosten; Hennie M.J. Roelofs; Geert Wanten

BACKGROUND Saturated medium-chain triglycerides (MCT) as part of the parenteral lipid regimen (50% MCT and 50% long chain triglycerides (LCT)) activate the immune system in vitro. Fish oil (FO)-derived n-3 fatty acids (FA) inhibit saturated FA-induced immune activation via a toll-like receptor (TLR)-4 mediated mechanism. We hypothesized that effects of parenteral MCTs on immune cells involve TLR-4 signaling and that these effects are modulated by n-3 FA that are present in FO. MATERIALS AND METHODS To test this hypothesis we assessed effects of addition of various commercially available mixed parenteral lipid emulsions, n-3 FA and of TLR-4 inhibition on MCT-induced human immune cell activation by evaluation of the expression of leukocyte membrane activation markers and reactive oxygen species (ROS) production. RESULTS All MCT-containing lipid emulsions activated leukocytes by inducing changes in expression of membrane markers and stimulus induced ROS production, whereas MCT-free lipid emulsions lacked this effect. Moreover, addition of n-3 FA to LCT/MCT did not prevent MCT-induced immune activation. TLR-4 inhibitors did not distinctly modulate MCT-induced changes in immune function. CONCLUSION Taken together, these findings suggest that leukocyte activation by parenteral MCTs does not involve TLR-4 signaling and is not modulated by n-3 FA in FO-, but is exerted via different signaling pathways.


Clinical Nutrition | 2016

Presentation of a nationwide multicenter registry of intestinal failure and intestinal transplantation

Esther Neelis; Anne Margot C. Roskott; Gerard Dijkstra; Geert Wanten; Mireille J. Serlie; Merit M. Tabbers; Gerard Damen; E.D. Olthof; C.F. Jonkers; J.H. Kloeze; Rutger J. Ploeg; Floris Imhann; Vincent B. Nieuwenhuijs; Edmond Rings


Journal of Parenteral and Enteral Nutrition | 2015

Do We Know the Benefits of a Taurolidine Lock in Adult Home Parenteral Nutrition Patients With a Low Infection Rate

E.D. Olthof; Mark M. Broekman; Geert Wanten


Clinical Nutrition | 2014

PP228-SUN: Taurolidine Lock is Superior to Heparin Lock in the Prevention of Catheter Related Bloodstream Infections and Occlusions

E.D. Olthof; M.W.J. Versleijen; G.J. Huisman-de Waal; Ton Feuth; W. Kievit; Geert Wanten


Clinical Nutrition | 2013

PP047-MON MEDIUM-CHAIN TRYGLICERIDE INDUCED LEUKOCYTE ACTIVATION IS NOT MEDIATED BY TOLL-LIKE RECEPTOR 4

E.D. Olthof; A.F. Gülich; L.A. Joosten; H.M. Schaap-Roelofs; Geert Wanten


Clinical Nutrition | 2013

PP201-SUN LONG-TERM TAUROLIDINE LOCK THERAPY IS MORE EFFECTIVE IN PREVENTING CATHETER RELATED BLOODSTREAM INFECTIONS IN ADULT HOME PARENTERAL NUTRITION PATIENTS THAN HEPARIN: A FOLLOW-UP OF 212 PATIENTS

E.D. Olthof; G.J. Huisman; M.W.J. Versleijen; Wietske Kievit; Geert Wanten

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Geert Wanten

Radboud University Nijmegen

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A.F. Gülich

Radboud University Nijmegen

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M.W.J. Versleijen

Radboud University Nijmegen Medical Centre

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Ton Feuth

Radboud University Nijmegen

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Wietske Kievit

Radboud University Nijmegen

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Anne Margot C. Roskott

University Medical Center Groningen

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