E. Giovenale

Cytogenetic observations in human peripheral blood leukocytes following in vitro exposure to THz radiation: a pilot study.

Emerging technologies are considering the possible use of Terahertz radiation in different fields ranging from telecommunications to biology and biomedicine. The study of the potential effects of Terahertz radiation on biological systems is therefore an important issue in order to safely develop a variety of applications. This paper describes a pilot study devoted to determine if Terahertz radiation could induce genotoxic effects in human peripheral blood leukocytes. For this purpose, human whole blood samples from healthy donors were exposed for 20 min to Terahertz radiation. Since, to our knowledge, this is the first study devoted to the evaluation of possible genotoxic effects of such radiation, different electromagnetic conditions were considered. In particular, the frequencies of 120 and 130 GHz were chosen: the first one was tested at a specific absorption rate (SAR) of 0.4 mW g−1, while the second one was tested at SAR levels of 0.24, 1.4, and 2 mW g−1. Chromosomal damage was evaluated by means of the cytokinesis block micronucleus technique, which also gives information on cell cycle kinetics. Moreover, human whole blood samples exposed to 130 GHz at SAR levels of 1.4 and 2 mW g−1 were also tested for primary DNA damage by applying the alkaline comet assay immediately after exposure. The results obtained indicate that THz exposure, in the explored electromagnetic conditions, is not able to induce either genotoxicity or alteration of cell cycle kinetics in human blood cells from healthy subjects.

The SPARC project: a high-brightness electron beam source at LNF to drive a SASE-FEL experiment

Abstract The Project Sorgente Pulsata e Amplificata di Radiazione Coerente (SPARC), proposed by a collaboration among ENEA–INFN–CNR–Universita’ di Tor Vergata–INFM–ST, was recently approved by the Italian Government and will be built at LNF. The aim of the project is to promote an R&D activity oriented to the development of a coherent ultra-brilliant X-ray source in Italy. This collaboration has identified a program founded on two main issues: the generation of ultra-high peak brightness electron beams and of resonant higher harmonics in the SASE-FEL process, as presented in this paper.

Compact free electron lasers: From Cerenkov to waveguide free electron lasers

Abstract The basic principles of free electron generators of coherent radiation and the practical considerations related to the development of far infrared and sub-millimeter wave sources are discussed in this paper. The attention is focused on compact devices, which reduce the size and cost issue. A review of the main results obtained at the Frascati FEL Facility is presented together with the planned experiments.

Coherence effects in FEL radiation generated by short electron bunches

Abstract In the last few years there has been a growing interest in the study of coherent radiation from short electron bunches. The possibility of realizing compact FEL devices in the far-infrared and millimeter wave regions utilizing small to medium size radio-frequency (RF) accelerators has stimulated a number of theoretical and experimental investigations to clarify basic physics issues in this process. Coherent emission experiments will be reviewed in this paper. Measurements on the temporal and spectral characteristics of the coherent emission from a single bunch and on the coherence between consecutive bunches will be presented along with an analysis of the influence of coherence on the dynamics of the laser.

Conceptual design of a high-brightness linac for soft X-ray SASE-FEL source

Abstract FELs based on SASE are believed to be powerful tools to explore the frontiers of basic sciences, from physics to chemistry to biology. Intense R&D programs have started in the USA and Europe in order to understand the SASE physics and to prove the feasibility of these sources. The allocation of considerable resources in the Italian National Research Plan (PNR) brought about the formation of a CNR–ENEA–INFN–University of Roma “Tor Vergata” study group. A conceptual design study has been developed and possible schemes for linac sources have been investigated, leading to the SPARX proposal. We report in this paper the results of a preliminary start to end simulation concerning one option we are considering based on an S-band normal conducting linac with high-brightness photoinjector integrated in an RF compressor.

Long wavelength compact-FEL with controlled energy–phase correlation

Abstract A radio-frequency modulated electron beam passing through a magnetic undulator generates coherent spontaneous emission (CSE) when the electron bunch length is comparable to the FEL resonant wavelength. At long wavelengths, CSE can be significantly enhanced by a proper ramping of the electron energy within the bunch duration, since this allows the contributions of the individual electrons to be added in phase. In this paper, the physical principles of the coherent spontaneous emission are reviewed together with its main experimental features. An accelerating structure, constructed at ENEA-Frascati for the systematic investigation of energy–phase correlation effects, is described together with an analysis of the expected performance.

THz-ARTE: non-invasive terahertz diagnostics for art conservation

The project THz-ARTE ldquoTerahertz Advanced Research TEchniques for non-invasive analysis in art conservationrdquo has recently been selected in the area of Technologies applied to Cultural Heritage in the framework of the Agreement between the Government of Italy and the Government of Japan on cooperation in Science and Technology.

Absorption and Diffusion Measurements of Biological Samples using a THz Free Electron Laser

A compact THz Free Electron Laser (FEL) isbeing used to perform irradiation ofbiological samples to investigate possiblegenotoxic effects. In order to evaluate theexact radiation dose absorbed by the singlecomponents of the samples it is necessaryto study the optical properties of thesamples, separating the contributions tothe radiation attenuation coefficientcoming from absorption and from diffusion.Spectroscopic measurements have beenperformed on different biological samples, comparing the experimental results withtheoretical models.

Biological effects of in vitro THz radiation exposure in human foetal fibroblasts

In recent years, terahertz (THz) radiation has been widely used in a variety of applications: medical, security, telecommunications and military areas. However, few data are available on the biological effects of this type of electromagnetic radiation and the reported results, using different genetic or cellular assays, are quite discordant. This multidisciplinary study focuses on potential genotoxic and cytotoxic effects, evaluated by several end-points, associated with THz radiation. For this purpose, in vitro exposure of human foetal fibroblasts to low frequency THz radiation (0.1-0.15THz) was performed using a Compact Free Electron Laser. We did not observe an induction of DNA damage evaluated by Comet assay, phosphorylation of H2AX histone or telomere length modulation. In addiction, no induction of apoptosis or changes in pro-survival signalling proteins were detected. Moreover, our results indicated an increase in the total number of micronuclei and centromere positive micronuclei induction evaluated by CREST analysis, indicating that THz radiation could induce aneugenic rather than clastogenic effects, probably leading to chromosome loss. Furthermore, an increase of actin polymerization observed by ultrastructural analysis after THz irradiation, supports the hypothesis that an abnormal assembly of spindle proteins could lead to the observed chromosomal malsegregation.

The SPARC/X SASE-FEL Projects

SPARC and SPARX are two different initiatives toward an Italian Free Electron Laser ~FEL! source operating in the Self Amplified Spontaneous Emission ~SASE! mode, in which several national research institutions are involved. SPARC is a high gain FEL project devoted to provide a source of visible and VUV radiation while exploiting the SASE mechanism. An advanced Photo-Injector system, emittance compensating RF-gun plus a 150 MeV Linac, will inject a high quality e-beam into the undulator to generate high brilliance FEL radiation in the visible region at the fundamental wavelength, ~;500 nm!. The production of flat top drive laser beams, high peak current bunches, and emittance compensation scheme will be investigated together with the generation of higher harmonic radiation in the VUV region. SPARX is the direct evolution of such a high gain SASE FEL toward the 13.5 and 1.5 nm operating wavelengths, at 2.5 GeV. To get the required value for the bunch peak current, Ipeak ’ 2.5 kA, the “hybrid” scheme, RF-compression stage plus magnetic chicane, is analyzed and compared with the more standard double stage of magnetic compression. The two options are reviewed considering the tolerance to the drive laser pulse phase jitter.