E. Kiarie

Energy and amino acid utilization in expeller-extracted canola meal fed to growing pigs.

Two experiments were conducted to determine the nutritive value of expeller-extracted canola meal (EECM) for growing pigs. In Exp. 1, a total of 6 ileally cannulated barrows (average initial BW = 26.8 kg) were fed 3 diets in a replicated 3 x 3 Latin square design to determine the apparent and standardized ileal digestibility (SID) values of N and AA and the SID AA contents of EECM. The 3 diets were a cornstarch-based diet with either solvent-extracted canola meal (SECM) or EECM as the sole source of protein, and a low-casein cornstarch-based diet, which was used to estimate basal endogenous N and AA losses to determine the SID of N and AA. All 3 diets contained chromic oxide as an indigestible marker for determining nutrient digestibility by the indicator method. In Exp. 2, a total of 18 intact barrows (average initial BW = 25.9 kg) were fed 3 diets in a completely randomized design (6 pigs per diet) to determine apparent total tract digestibility and retention of nutrients and the DE and ME contents of EECM. The diets were a basal corn-based diet or the basal diet with corn replaced by 35% SECM or EECM. The basal diet was used for determining the total tract digestible nutrient content by the difference method. Solvent-extracted canola meal, which is commonly used in the formulation of swine diets, was fed in both experiments for comparison with EECM. The SECM and EECM were similar in CP content (41.8 vs. 41.4%). Expeller-extracted canola meal was, however, greater in ether extract content (12.03 vs. 5.54%) and decreased in NDF content (23.8 vs. 29.9%) compared with SECM. The EECM also had a greater content of all the AA except Met, Cys, and Ser, by approximately 6.6%; Cys was greater in SECM, whereas Met and Ser were similar between the 2 meals. The EECM had greater (P < 0.05) SID of N, Arg, Ile, Leu, Phe, Glu, and Pro. The SID contents of Arg, His, Ile, Leu, Phe, Val, Ala, Asp, Glu, Gly, Pro, and Tyr were also greater (P < 0.05) for EECM than for SECM by an average of 15%. The EECM had greater (P < 0.01) DE (4,107 vs. 3,790 kcal/kg) and ME (3,978 vs. 3,564 kcal/kg) values compared with SECM. The results show that the EECM used in the current study had greater digestible AA and energy and a greater ME content than the SECM; hence, it may be a better source of protein and energy for growing pigs than SECM.

Growth performance and gastrointestinal microbial ecology responses of piglets receiving Saccharomyces cerevisiae fermentation products after an oral challenge with Escherichia coli (K88)1

The effects of Saccharomyces cerevisiae fermentation products (YFP) on growth performance and gastrointestinal (GIT) microbial ecology in 90 weanling pigs orally challenged with Escherichia coli K88(+) (ETEC) were investigated. The YFP were an original YFP product (XPC) and a water-suspendable yeast fermentation prototype (WSYFP) from a commercial company. Treatments consisted of a negative control (NC, no in-feed or in-water additive), carbadox (AB, 55 mg of carbadox/kg of feed), XPC (in feed, 0.2%), and WSYFP (in water, 0.5, 1, or 2 g/pig per day), and each was allotted to 5 pens (3 pigs/pen). The diets met the 1998 NRC specifications. Pigs were acclimated to treatments for a 7-d period before an ETEC challenge. On d 8, blood was collected from pigs to determine the baseline packed cell volume (PCV) measurement, and pigs were orally challenged with ETEC. At various time points postchallenge, blood samples were taken, performance measures and fecal consistency scores were recorded, and gut digesta and tissue samples were taken to evaluate GIT morphology, microbial ecology, and metabolites. Preplanned contrasts were used for comparison. Pigs receiving YFP had greater ADFI than NC pigs on d 3 (424 vs. 378 g/d; P = 0.01) and d 7 (506 vs. 458 g/d; P = 0.03) postchallenge. This effect of YFP on ADFI was similar to that of AB on d 3, but pigs receiving AB ate more (576 vs. 506 g/d; P = 0.03) at d 7 than pigs receiving YFP. Pigs exhibited reduced (P < 0.001) PCV upon ETEC challenge; however, pigs receiving additives sustained a greater (P < 0.05) PCV at 72 h compared with the NC group. Compared with the NC pigs, pigs receiving YFP showed a smaller (P < 0.05) number of ileal mucosa adherent ETEC and prevalence of the order Enterobacteriales in the ileal digesta, which corresponded to less (5.09 vs. 6.97 mg/dL; P = 0.03) colonic ammonia on d 7 postchallenge. Most of the indices for ileal digesta bacterial richness and diversity were greater (P < 0.01) for YFP pigs compared with NC pigs. However, results also indicated that the influence of YFP on the piglet intestinal microenvironment might differ when given in feed or water during ETEC challenge. In conclusion, pigs receiving YFP showed a better appetite in the presence of ETEC, which, together with the greater ileal digesta bacteria richness and diversity and decreased ETEC adhering to the mucosa and reduced colonic ammonia, indicates a healthier GIT environment.

Amino acid and fiber digestibility, intestinal bacterial profile, and enzyme activity in growing pigs fed dried distillers grains with solubles-based diets

The aim of this study was to determine ileal AA and fiber digestibility in new-generation dried distillers grains with solubles (DDGS) derived from wheat (wDDGS), a wheat-corn blend [wcDDGS, wheat and corn were fermented in a 7:3 wt/wt ratio], or corn (cDDGS) and to determine the effects of diets containing DDGS on gut bacteria and bacterial and digestive enzyme activities. Experimental diets contained one of the DDGS samples as the sole source of protein, and a low protein diet (5% casein) was included to estimate basal endogenous ileal CP and AA losses. Chromic oxide (0.3%) was added as an indigestible marker to all diets. Twelve cannulated barrows with an initial BW of 20.2 +/- 1.3 kg were allotted to the 4 experimental diets in a 2-period crossover design, which provided 6 observations per diet. Pigs were acclimatized to their diets for 5 d followed by a continuous 12-h digesta collection on d 6 and 7. Diet had no effect on the apparent ileal digestibility (AID) of CP (P = 0.58). The wDDGS diet generally had decreased (P < 0.05) AID of AA compared with the wcDDGS or cDDGS diet. Similarly, the values for standardized ileal digestibility of CP and most AA were smaller (P < 0.05) for the wDDGS diet compared with the other 2 DDGS diets. The Lys and Thr were the least digestible AA among the indispensable AA across the 3 DDGS samples. The digestibilities of nonstarch polysaccharides and NDF were not affected by diet (P = 0.80 and 0.40, respectively); however, the ileal digesta viscosity was greater (P < 0.05) for the wcDDGS diet than the wDDGS and cDDGS diets. The counts of Lactobacillus (P = 0.09) and Enterobacteriaceae (P = 0.05) were greater for the cDDGS diet compared with the other 2 diets. Accordingly, the cDDGS diet elicited a greater (P < 0.05) lactic acid concentration in digesta than the wDDGS diet. The activities of bacterial (P = 0.86 to 0.91) and digestive enzymes (P = 0.31 to 0.80) did not differ among the diets. The results indicate that the wDDGS had generally less protein and AA ileal digestibilities compared with the wcDDGS and cDDGS samples and that nonstarch polysaccharides and NDF digestibilities were similar among diets. Although diet influenced digesta bacterial counts, no effects were observed on the activities of bacterial and digestive enzymes.

Net energy of diets containing wheat-corn distillers dried grains with solubles as determined by indirect calorimetry, comparative slaughter, and chemical composition methods

The NE content of diets containing wheat-corn distillers dried grains with solubles (wcDDGS; 1:1 ratio) fed to growing pigs was determined using the comparative slaughter (CS), indirect calorimetry (IC), and chemical composition (CH) methods. The experimental diets were a corn-soybean meal control diet (CTRL), CTRL + 15% wcDDGS, and CTRL + 30% wcDDGS. In Exp. 1, 56 barrows (18.5 kg BW) were used to determine the NE value of diets using the CS method. Pigs were initially placed in 8 groups (7/group), based on BW and 1 pig/group was killed at the start of the experiment to obtain baseline body composition. The remaining 48 pigs were housed in pairs and allotted to the 3 diets (n = 8). Pigs had free access to feed and water for a 28-d period, after which 1 pig/pen was slaughtered to determine final body composition. Based on the CS method, NE values of 2,430, 2,427, and 2,429 kcal/kg DM were obtained for diets containing 0%, 15%, and 30% wcDDGS, respectively. In Exp. 2, 18 barrows (20.4 kg BW) were used to determine the NE value of diets using the IC and CH methods. Pigs were individually housed in metabolism crates and fed the 3 diets (n = 6) at 550 kcal ME/kg BW/d for a 16-d period. Feces and urine were collected from d 11 to 16, followed by measurement of O(2) consumption, CO(2) production, and urinary N, over a 36-h period using an IC system. For the IC method, NE values of 2,586, 2,513, and 2,520 kcal/kg DM were obtained for diets containing 0%, 15%, and 30% wcDDGS, respectively, and corresponding values for the CH method were 2,447, 2,451, and 2,368 kcal/kg DM, respectively. The NE values that were obtained with the CS, IC, and CH methods were not different.

Effect of a carbohydrase mixture on ileal amino acid digestibility in extruded full-fat soybeans fed to finishing pigs 1 2

Extrusion technology has been used successfully to improve the nutritive value of full-fat oilseeds via shear force and heat applied during passage through the extruder, exposing more peptide bonds to enzymatic hydrolysis. However, the fibrous portion of the seeds is largely intact after extrusion. Therefore, application of carbohydrase mixtures targeting nonstarch polysaccharides might further improve the nutritive value of extruded full-fat oilseeds. This hypothesis was investigated in a study involving 6 ileal-cannulated barrows (average initial BW = 75.0 kg) fed extruded full-fat, soybean- (FFSB) based diets, without or with a carbohydrase mixture (CM) to determine apparent ileal digestibility (AID) of energy and standardized ileal digestibility (SID) of AA. The CM supplement provided 500, 50, 400, 1200, 450, and 45 units of pectinase, cellulase, mannanase, xylanase, glucanase, and galactanase, respectively, per kilogram of diet. A basal diet was formulated to contain FFSB as the sole source of AA, whereas the CM diet was formulated by supplementing the basal diet with CM. Diets contained titanium dioxide as an indigestible marker. Diets were fed in a 2-period crossover design to give 6 observations per diet. Each experimental period lasted 7 d, including a 5-d adaptation period and 2-d ileal digesta collection period. The SID of N and AA were calculated using published values for ileal endogenous N and AA losses from our laboratory. Enzyme supplementation increased (P < 0.01) AID values of DM (68.7 vs. 65.7%), GE (74.6 vs. 70.5%), and N (64.2 vs. 58.5%). Greater (P < 0.05) AID values were recorded for Leu, Met + Cys, Thr, Cys, Ser, and Tyr in the CM diet compared with the basal diet. Compared with the basal diet, the CM diet had greater SID values for N (73.7 vs. 68.6%; P < 0.01), Met + Cys (59.9 vs. 52.2%; P < 0.05), and Thr (66.3 vs. 61.2%; P < 0.05). The average SID for indispensable and total AA increased by 3.4% and 3.8% units, respectively, after enzyme supplementation. In conclusion, enzyme supplementation increased the nutritive value of extruded FFSB for finishing pigs.

Standardized ileal amino acid digestibility in egg from hyperimmunized hens fed to weaned pigs.

The study was conducted to determine the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of AA in egg from hens hyperimmunized with Escherichia coli K88 antigens (EGG) fed to weaned pigs. Spray dried porcine plasma (SDPP) was included for comparison. Eight barrows (Yorkshire-Landrace × Duroc; initial BW of 17 ± 1 kg) were fitted with a T-cannula at the distal ileum and fed 2 diets in a completely randomized design to give 4 replicates per diet. The diets were corn (Zea mays) starch based with either EGG or SDPP as the sole source of protein and were formulated to contain 130 g/kg CP. At the end of the study, a 50 g/kg casein diet was fed to all pigs (n = 8) to quantify endogenous N and AA losses to determine SID. Titanium dioxide (3 g/kg) was included in the diets as an indigestible maker. Each period lasted for 7 d. Pigs were adapted to their respective diets for 5 d followed by 12 h of continuous ileal digesta collection on days 6 and 7. Daily feed allowance was set at 4% BW at the beginning of each period and offered in 2 equal portions at 0800 and 1600 h as a dry mash. Pigs had unlimited access to water via low pressure nipple drinkers. The AID (%) of CP and indispensable AA were lower (P < 0.05) in EGG compared with SDPP. The SID (%) of CP (82 vs. 96) and indispensable AA were lower (P < 0.05) in pigs fed EGG compared with SDPP. In conclusion, the average AID and SID of N and indispensable AA in EGG are lower than in SDPP when fed at high levels.

Effect of products derived from hydrolysis of wheat and flaxseed non starch polysaccharides by carbohydrase enzymes on net absorption in enterotoxigenic Escherichia coli (K88) challenged piglet jejunal segments

Enterotoxigenic Escherichia coli (ETEC) infection results in fluid and electrolyte losses in the small intestine. We investigated the effect of non-starch polysaccharides (NSP) hydrolysis products of wheat middlings (WM) and flaxseed (FS) on net absorption of fluid and solutes during ETEC challenge. Products were generated by incubating WM and FS with a blend of carbohydrase enzymes to produce 2 products: 80% ethanol-soluble (ES) and 80% ethanol-insoluble (EI) which were studied in 2 experiments in which 2 factors were investigated: products (EI vs. ES) and time of ETEC challenge (before vs. after perfusion). Pairs of small-intestine segments, one non-challenged and the other ETEC-challenged were perfused with products for 7.5 h. ETEC reduced fluid absorption by more than 380 microL/cm(2) in saline (control) perfused segments, whereas this reduction was significantly (P < 0.05) less for the WM and FS products. Interaction (P > 0.05) between product and time of challenge was not observed. For WM, products effects on ETEC-challenged segments were such that perfusion of ES resulted in higher total solute (measured as osmolality) absorption than EI (138 vs. 103 microOsmol/cm(2)). In conclusion, hydrolysis products from WM and FS were beneficial in maintaining fluid balance during ETEC challenge, suggesting potential in controlling ETEC induced diarrhea in piglets.

Effects of a multi-strain Bacillus species-based direct-fed microbial on growth performance, nutrient digestibility, blood profile, and gut health in nursery pigs fed corn-soybean meal-based diets.

This experiment was conducted to investigate the effect of a spp.-based direct-fed microbial (DFM) on growth performance, apparent total tract digestibility (ATTD), blood profile, intestinal histomorphology, and fecal gas emission in piglets fed corn and soybean meal-based diets. The DFM product was based on 1 strain of and 2 strains of and formulated to supply 1.5 × 10 cfu/g of feed. A total of 128 piglets ([Yorkshire × Landrace] × Duroc; 6.8 ± 0.6 kg BW; weaning age: 24 d) were housed in groups (4 pigs/pen, 2 barrows and 2 gilts) and fed diets ( = 16) without or with DFM in a 2-phase feeding program: d 0 to 14 (phase I) and 15 to 42 (phase II). Feed intake and BW were measured weekly. At the end of each phase, samples for blood urea nitrogen (BUN), blood creatinine, ATTD, and fecal noxious gas emission were taken. At termination, 12 piglets per treatment were killed to access intestinal tissues for histomorphology. Overall, pigs fed DFM had a greater ( < 0.05) G:F than pigs fed the control diet. In phase I, pigs fed DFM showed a greater ( < 0.05) ADG and lower ( < 0.05) concentration of BUN and fecal ammonia emission than the control group. In phase II, a greater ( < 0.05) ATTD of nitrogen and longer ( < 0.05) duodenum and jejunum villi were observed in pigs fed the DFM diet compared with the control group. In conclusion, inclusion of DFM improved growth performance and villi length of the duodenum and jejunum in nursery pigs. Furthermore, DFM enhanced protein utilization as demonstrated by increased nitrogen digestibility, lower BUN, and lower fecal ammonia release.

Amino acids and energy utilization in zero-tannin faba bean and co-fermented wheat and corn dried distillers grains with solubles fed to growing pigs1

Application of alternative feedstuffs in swine diets will be dependent on characterization of their nutritive value. In 2 experiments, we determined the AA and energy utilization of Manitoba-grown zero-tannin faba beans (ZTFB) and co-fermented wheat and corn dried distillers grains with solubles (wcDDGS). Corn dried distillers grains with solubles (cDDGS) was also included for comparison. In Exp. 1, 6 ileal cannulated barrows (29.3 ± 1.3 kg) were fed 3 diets in a replicated 3 × 3 Latin square design to determine the apparent (AID) and standardized (SID) ileal digestibility of AA. The 3 diets contained ZTFB, wcDDGS, or cDDGS as the sole source of AA. The SID of N and AA were calculated using published values for ileal endogenous N and AA losses from our laboratory. In Exp. 2, 12 intact barrows (22.5 ± 1.1 kg) were fed 4 diets in a two 15-d period crossover design to determine DE and ME contents of the test ingredients by difference method. The diets were a basal corn-based diet or the basal diet with corn replaced by 46% ZTFB, wcDDGS, or cDDGS. The concentrations (DM basis) of GE (kcal/kg), CP (%), and Lys (%) in ZTFB were 4,144, 27, and 1.6, respectively, and corresponding values for wcDDGS were 5,112, 31, and 0.8. The ZTFB had greater (P < 0.05) SID of Lys (87%) compared with wcDDGS (72%) and cDDGS (68%); as a result, the SID content (g/kg DM) of Lys in ZTFB (13.5) was greater (P < 0.05) than that of either wcDGGS (6.03) or cDDGS (6.68). The dried distillers grains with solubles (DDGS) samples had greater (P < 0.05) SID and contents of sulfur AA (Met and Cys) compared with ZTFB. The ME content (kcal/kg DM) of ZTFB (3,548) was less (P < 0.05) than that of cDDGS (3,851) whereas the ME content of wcDDGS (3,669) was similar to that of ZTFB or cDDGS. The results showed that ZTFB has greater digestible Lys whereas wcDDGS has greater digestible sulfur AA but both ingredients have comparable ME content. Therefore, a blend of these 2 ingredients can serve as an excellent source of AA and energy for swine.

Growth performance and nutrient digestibilities in nursery pigs receiving varying doses of xylanase and β-glucanase blend in pelleted wheat- and barley-based diets

In 2 experiments, dose response efficacy of a xylanase and β-glucanase blend (XB) on growth performance and ileal nutrient digestibility was investigated in nursery pigs fed pelleted wheat (Triticum aestivum)- and barley (Hordeum vulgare)-based diets. A basal diet (meeting NRC [1998] specifications for 6 to 30 kg BW, except for 5% lower DE) was supplemented with XB to give 4 diets (0, 50, 100, and 200 g/t). The xylanase and β-glucanase blend was formulated to contain guaranteed activity of 12,200 and 1520 units/g, respectively. In Exp.1, 192 nursery pigs (initial BW of 6.5 kg) were randomly assigned to 4 diets to give 12 pens (4 pigs/pen) per diet to study growth performance for 42 d. In Exp. 2, apparent ileal digestibility (AID) of energy and AA was evaluated in 4 individually housed ileal-cannulated barrows (21 kg BW) according to a 4 × 4 Latin square design. In Exp. 1, XB linearly and quadratically increased (P < 0.05) G:F compared with control. Adding 200 g/t increased overall G:F by 20% compared with control. In Exp. 2, XB linearly increased (P < 0.05) AID of DM, CP, energy, and AA. In conclusion, supplemental xylanase and β-glucanase in nursery pelleted wheat- and barley-based diets deficient in DE increased energy and nutrient use, resulting in better G:F. In conclusion, an enzyme product containing a combination of xylanase and β-glucanase allowed young pigs to derive more nutrients and energy in a wheat- and barley-based diet deficient in energy.