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Dive into the research topics where E. Sócrates T. Egito is active.

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Featured researches published by E. Sócrates T. Egito.


International Journal of Pharmaceutics | 2013

Development of oil-in-water microemulsions for the oral delivery of amphotericin B

Acarília Eduardo da Silva; Gillian Barratt; Monique Chéron; E. Sócrates T. Egito

Amphotericin B (AmB) is a very efficient drug against serious diseases such as leishmaniasis and systemic fungal infections. However, its oral bioavailability is limited due to its poor solubility in water. Nevertheless, it is marketed as high-cost lipid parenteral formulations that may induce serious infusion-related side effects. In this study, oil-in-water (O/W) microemulsions (MEs) were developed and characterized with a view to their use as solubility enhancers and oral delivery systems for AmB. Therefore, different nonionic surfactants from the Tween(®) and Span(®) series were tested for their solubilization capacity in combination with several oils. Based on pseudoternary phase diagrams, AmB-loaded MEs with mean droplet sizes about 120 nm were successfully produced. They were able to improve the drug solubility up to 1000-fold. Rheological studies showed the MEs to be low-viscosity formulations with Newtonian behavior. Circular dichroism and absorption spectra revealed that part of the AmB in the MEs was aggregated as an AmB reservoir carrier. Cytotoxicity studies revealed limited toxicity to macrophage-like cells that may allow the formulations to be considered as suitable carriers for AmB.


Aaps Pharmscitech | 2006

Micro-emultocrit technique: a valuable tool for determination of critical HLB value of emulsions.

Janus P. F. Macedo; Leonardo L. Fernandes; Fábio R. Formiga; Michael F. Reis; Toshiyuiky Nagashima Júnior; Luiz Alberto Lira Soares; E. Sócrates T. Egito

The aim of this work was to develop a methodology for rapid determination of the critical hydrophilic-lipophilic balance (HLB) value of lipophilic fractions of emulsions. The emulsions were prepared by the spontaneous emulsification process with HLB value from 4.3 to 16.7. The preparations were stored at 2 different temperatures (25°C and 4°C) and their physicochemical behavior was evaluated by the micro-emultocrit technique and the long-term stability study. The experimental data show a reverse relationship between HLB values of the surfactant mixtures and emulsion stability. A close correlation between the results for both stability procedures was observed, suggesting the use of micro-emultocrit to predict stabilities of such systems. In addition, it was found that the critical HLB of the Mygliol 812 was 15.367.The aim of this work was to develop a methodology for rapid determination of the critical hydrophilic-lipophilic balance (HLB) value of lipophilic fractions of emulsions. The emulsions were prepared by the spontaneous emulsification process with HLB value from 4.3 to 16.7. The preparations were stored at 2 different temperatures (25°C and 4°C) and their physicochemical behavior was evaluated by the micro-emultocrit technique and the long-term stability study. The experimental data show a reverse relationship between HLB values of the surfactant mixtures and emulsion stability. A close correlation between the results for both stability procedures was observed, suggesting the use of micro-emultocrit to predict stabilities of such systems. In addition, it was found that the critical HLB of the Mygliol 812 was 15.367.


Journal of Drug Targeting | 2010

Pharmaceutical emulsions: a new approach for gene therapy

Lourena Mafra Verissimo; Lucymara Fassarela Agnez Lima; Lucila Carmem Monte Egito; Anselmo Gomes de Oliveira; E. Sócrates T. Egito

The concept of gene therapy involves the experimental transfer of a therapeutic gene into an individual’s cells and tissues to replace an abnormal gene aiming to treat a disease, or to use the gene to treat a disease just like a medicine, improving the clinical status of a patient. The achievement of a foreigner nucleic acid into a population of cells requires its transfer to the target. Therefore, it is essential to create carriers (vectors) that transfer and protect the nucleic acid until it reaches the target. The obvious disadvantages of the use of viral vectors have directed the research for the development of a nonviral organized system such as emulsions. In fact, recently, there has been an increase of interest in its use in biotechnology as a nonviral vector for gene therapy. This review focuses on the progress of cationic emulsions and the improvement of the formulations, as a potential delivery system for gene therapy.


Aaps Pharmscitech | 2008

Influence of the Lipophilic External Phase Composition on the Preparation and Characterization of Xylan Microcapsules—A Technical Note

Toshiyuki Nagashima; Elquio Eleamen Oliveira; Acarília Eduardo da Silva; Henrique Rodrigues Marcelino; Monique Christine Salgado Gomes; Larissa Muratori Aguiar; Ivonete B. Araújo; Luiz Alberto Lira Soares; Anselmo Gomes de Oliveira; E. Sócrates T. Egito

Scientific studies on new drug delivery systems have significantly increased in the past few years and this growth is expected to continue in the near future (1). Such systems are of great interest because of their ability to improve drug performance in terms of efficacy, safety, and patient compliance (1). In many cases, conventional drug delivery provides an increase of drug concentration at potentially toxic levels (2). Additionally, the need for delivering drugs with fewer side effects has prompted the development of new drug delivery systems (1). Xylan is the second most abundant polymer found in hardwoods and annual plants (3), particularly in agricultural residues such as grain hulls, corn cobs, and corn stalks (4). Depending on the botanical source, the backbone chemical structure may vary. However, the majority of xylans present side chains of different sugars such as 4-O-methyl-d-glucoronic acid, O-acetyl-l-arabinose, l-arabinose, and d-glucoronic acid bond by a glycosidic linkage to the backbone (3). Because of its complex structure, the complete degradation of xylan requires the activity of several enzymes which are specifically produced by human colonic microflora (5). Therefore, xylan microcapsules may represent a novel and promising colon-specific drug delivery system. Microcapsules based on natural polymers may be produced by means of a variety of methods. Emulsion solvent extraction, emulsion solvent evaporation and interfacial cross-linking polymerization are the most commonly employed processes for the production of microcapsules (4). One of the critical parameters in the microencapsulation process is the external phase used in the emulsification step (6). In fact, the external phase can influence the microcapsules morphology, their aggregation state, and mainly the release of the microencapsulated active compound (7). Because the production of xylan-based microcapsules is a subject barely studied by scientists worldwide, the aim of this work was to evaluate the influence of the lipophilic external phase composition on the production and mean particle size of xylan microcapsules produced by interfacial cross-linking polymerization.


Journal of Dispersion Science and Technology | 2009

Physicochemical Characterization and Development of a Microemulsion System for Transdermal Use

José Augusto P Silva; Danilo César Galindo Bedor; Bolivar P. G. L. Damasceno; Anselmo Gomes de Oliveira; E. Sócrates T. Egito; Davi Pereira de Santana

A microemulsion (ME) carrier system containing diclofenac diethylamine (DDA) was developed and physicochemically characterized. In addition, a methodology for analytical determination of DDA by UV-Vis spectrophotometry was validated. The methodology used to produce the ME was simple and reliable, producing a stable ME system with a high DDA entrapment efficiency of 89.6 ± 14.19%. The ME system presents a mean droplet size, conductivity, pH, refractive index and viscosity of around 50 nm, 300 µS cm−1, 7.0, 1.399, and 110 mPa, respectively. Furthermore, the analytical method developed to evaluate the DDA content was validated and used to quantify DDA in the ME formulations.


Drug Development and Industrial Pharmacy | 2008

Development of superparamagnetic microparticles for biotechnological purposes.

Amanda K. A. Silva; E. Sócrates T. Egito; Toshiyuki Nagashima-Júnior; Ivonete B. Araújo; Érica L. Silva; Luiz Alberto Lira Soares; A. S. Carriço

Aqueous suspensions containing magnetic microparticles have been increasingly used in biosciences and biotechnology. This work describes an experimental procedure to produce superparamagnetic microparticles. The particles were prepared based on the coprecipitation of iron salts in alkaline medium. Afterwards, characterization was performed. Characterization data demonstrated that magnetite was the dominant phase in the analyzed sample, and 50% of them were in the size range of 0.5–5μm. The results suggest that the experimental protocol provided a simple synthesis route to produce superparamagnetic microparticles. Such properties may be very useful for biotechnological purposes.


Current Drug Delivery | 2005

Decrease in Fungizone™ Toxicity Induced by the Use of Lipofundin™ As a Dilutent: An In Vitro Study

Ivonete B. Araújo; Bolivar Ponciano Goulart de Lima Damasceno; Tereza Maria Dantas de Medeiros; Luiz Alberto Lira Soares; E. Sócrates T. Egito

The aim of this work was to develop an in vitro experimental protocol for the evaluation of toxicity and efficacy of an amphotericin B (AmB) micelle system, Fungizone, which was previously diluted with a lipid based emulsion for parenteral use, named Lipofundin LCT/MCT-20%. Two cell models were used for the experiments: Red Blood Cells (RBC) from human donnors and Candida tropicalis (Ct). These models were used to perform the toxicity and activity of the Fungizone/ Lipofundin admixture (AmB-LP) and the Fungizone (AmB-M) alone. While potassium (K+) and hemoglobin leakage from RBC were the parameters used to evaluate the acute and chronic toxicity, respectively, the efficacy of AmB-LP and AmB-M were assessed by K+ leakage or cell survival rate (CSR) from Ct. The results show that the toxicity of AmB-LP to RBC was concentration dependent concerning the K+ leakage; while at high concentrations, 5 and 50 mg x mL(-1), the leakage was 50.91 +/- 2.09% and 95.71 +/- 0.64%, respectively, at a concentration of 0.5 mg x mL(-1) this value was 17.16 +/- 1.57% and the value tended to zero for the lowest concentration studied, 0.05 mg x mL(-1). Surprisingly, AmB-LP induced very low hemoglobin leakage for all concentrations studied. At the highest concentration, 50 mg x mL(-1), this value was around 3%. When the cell model was Ct, the results changed completely. Not only high concentrations of AmB-LP, but also lower ones were able to induce a K+ permeability of around 100%. The CSR parameter showed an inverse correlation with the concentration; high values, between 50 and 5 mg x mL(-1), resulted in a CSR of around 8%. On the other hand, for lower concentration values, 0.05 and 0.5 mg x mL(-1), this one was around 80%. The same profile of activity against Ct was found for AmB-M. Only a small variation was found for the K+ leakage at 0.05 mg x mL(-1) that presented a value of 96.99 +/- 2.53%. However, AmB-M seemed to be much more toxic than AmB-LP. Its induction of hemoglobin leakage started at 0.5 mg x mL(-1) and reached the 100% at 5 mg x mL(-1). K+ leakage results were worse. The intermediate concentrations of study, 0.5 and 5 mg x mL(-1), presented a significant increase compared to AmB-LP. All together these results reveal that the activity of AmB is not only concentration dependent, but also depends on the drug carrier in which this compound was inserted. The AmB-LP preparation showed the same efficacy as AmB-M, but with a low toxicity. Therefore, AmB-LP presented a higher therapeutic index that permits the administration of high concentration of AmB without revealing side effects. However, the simple mixture of two complex pharmaceutical entities, as micelles and emulsions, should be analyzed carefully to assure that physicochemical stability is not reduced and thereby cause a different biodistribution in vivo.


Current Drug Delivery | 2016

Development and Characterization of a Microemulsion System Containing Amphotericin B with Potential Ocular Applications.

Walteçá Louis Lima Silveira; Bolivar Ponciano Goulart de Lima Damasceno; Laura F. Ferreira; Izabel L.S. Ribeiro; Karolyne S. Silva; André Leandro Silva; Maria José Mendes Giannini; Arnóbio Antônio da Silva-Júnior; Anselmo Gomes de Oliveira; E. Sócrates T. Egito

BACKGROUND Amphotericin B eye drops are widely used in the treatment of ocular infections. However, amphotericins toxicity leads to low patient compliance and aggravation of symptoms. This work describes the development of a microemulsion system containing amphotericin B, aiming for its use in ocular applications. METHODS The microemulsion was developed by the titration technique. The physicochemical characteristics were determined with both loaded and unloaded amphotericin B-microemulsion. The nanostructures were analyzed by polarized light microscopy. The microdilution method was used to establish the minimum inhibitory concentration against fungal strains, and, therefore, evaluate the microemulsion activity. Additionally, in order to evaluate the microemulsion toxicity an in vitro toxicity assay against red blood cells was performed. RESULTS The performed studies showed that the presence of amphotericin B loaded into the system did not induce serious changes in the physicochemical properties of the microemulsion when compared to the unloaded system. The spectrophotometric studies depicted amphotericin B-self-associated species, which allow predicting its behavior in vitro. The high pressure liquid chromatography results revealed high drug content entrapment in the microemulsion droplet. Finally, the amphotericin B-microemulsion in vitro susceptibility test showed high activity against Candida strains and a low toxicity profile against red blood cells when compared to Fungizone®. CONCLUSION The physicochemical characterization of the microemulsion demonstrated that its characteristics are compatible with the topical ocular route, making it eligible for consideration as a new and interesting amphotericin B-deliverydosage form to be used as eye drop formulation.


Acta Cirurgica Brasileira | 2005

Similarity between the in vitro activity and toxicity of two different fungizone™ / lipofundin™ admixtures

Ivonete B. Araújo; C. Ramon N. Brito; Isabel A. Urbano; Victor A. Dominici; Miguel A. Silva Filho; Walteçá Louis Lima Silveira; Bolivar Ponciano Goulart de Lima Damasceno; Aldo Cunha Medeiros; E. Sócrates T. Egito

PURPOSE Amphotericin B (AmB), an antifungal agent that presents a broad spectrum of activity, remains the gold standard in the antifungal therapy. However, sometimes the high level of toxicity forbids its clinical use. The aim of this work was to evaluate and compare the efficacy and toxicity in vitro of Fungizon (AmB-D) and two new different AmB formulations. METHODS three products were studied: Fungizon, and two Fungizon /Lipofundin admixtures, which were diluted through two methods: in the first one, Fungizon was previously diluted with water for injection and then, in Lipofundin (AmB-DAL); the second method consisted of a primary dilution of AmB-D as a powder in the referred emulsion (AmB-DL). For the in vitro assay, two cell models were used: Red Blood Cells (RBC) from human donors and Candida tropicallis (Ct). The in vitro evaluation (K+ leakage, hemoglobin leakage and cell survival rate-CSR) was performed at four AmB concentrations (from 50 to 0.05 mg x L(-1)). RESULTS The results showed that the action of AmB was not only concentration dependent, but also cellular type and vehicle kind dependent. At AmB concentrations of 50 mg x L(-1), although the hemoglobin leakage for AmB-D was almost complete (99.51), for AmB-DAL and AmB-DL this value tended to zero. The p = 0.000 showed that AmB-D was significantly more hemolytic. CONCLUSION The Fungizon-Lipofundin admixtures seem to be the more valuable AmB carrier systems due to their best therapeutic index presented.


International Journal of Pharmaceutics | 2007

Synthesis and characterization of xylan-coated magnetite microparticles

Amanda K. Andriola Silva; Érica L. Silva; Elquio Eleamen Oliveira; Toshiyuki Nagashima; Luiz Alberto Lira Soares; Aldo Cunha Medeiros; Jose Humberto de Araujo; Ivonete B. Araújo; A. S. Carriço; E. Sócrates T. Egito

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Ivonete B. Araújo

Federal University of Rio Grande do Norte

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Luiz Alberto Lira Soares

Federal University of Rio Grande do Norte

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A. S. Carriço

Federal University of Rio Grande do Norte

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Aldo Cunha Medeiros

Federal University of Rio Grande do Norte

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Érica L. Silva

Federal University of Rio Grande do Norte

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Acarília Eduardo da Silva

Federal University of Rio Grande do Norte

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Amanda K. Andriola Silva

Federal University of Rio Grande do Norte

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Elquio Eleamen Oliveira

Federal University of Rio Grande do Norte

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Toshiyuki Nagashima

Federal University of Rio Grande do Norte

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