Earl G. Alley

Comparative study of 8-monohydromirex and mirex toxicity in male rats

Abstract Male rats were fed 0, 0.5, 5.0, 50, and 75 ppm mirex or 8-monohydromirex for 28 days. There were no consistent differences between weight gain of treated or control groups. There were significant increases in LW/BW in the 5, 50, and 75 ppm mirex and 8-monohydromirex treatment groups. Serum β-glucuronidase was elevated in the 0.5, 50, and 75 ppm mirex treated groups, and was elevated in the 5, 50, and 75 ppm 8-monohydromirex treated groups. There were no consistent changes in serum sorbitol dehydrogenase in any treatment group, but there were significant increases in liver sorbitol dehydrogenase at all levels for both toxicants. Hepatic microsomal parameters were induced by both compounds. No consistent toxic effects resulting from mirex or 8-monohydromirex treatment were indicated by serum glucose, protein, and cholesterol or by selected hematological factors. The histological changes observed in the livers of animals treated with mirex and 8-monohydromirex included cell swelling with increased cytoplasmic density; a dense homogenous region centered around the nuclear area; hyaline or myelin-like cytoplasmic inclusions appearing in some tissue sections of the highest treatment levels; and lipidosis. Histological changes in testes of treated rats were less obvious than lesions observed in the liver. There was a loss of staining intensity of the seminiferous tubules associated with hypocellularity of the tubules. Decreased spermatogenesis was evident in three rats from the high dose groups. Both compounds produced detectable histological changes in the thyroid glands at the 75 ppm treatment level. There did not appear to be any consistently significant differences between the toxic effects, either qualitative or quantitative, of mirex and 8-monohydromirex in rats fed either compound for 28 days.

Derivatization of diaminocyclohexanes for determination of cis and trans isomers by gas chromatography

Abstract Derivatives of the cis and trans isomers of 1,2-, 1,3- and 1,4-diaminocyclohexanes were prepared by the reaction of the amine with benzaldehyde. These compounds were analyzed by gas chromatography on a DB-5, bonded phase, fused-silica capillary column resulting in a baseline separation for the six isomers providing accurate determination of the cis-to-trans ratios in a single gas chromatographic analysis. Derivatives of other amine compounds were prepared to evaluate possible interferences.

Vibrational frequency patterns for hydrogen-substituted degradation products of the pesticide mirex

Abstract A complete vibrational study has been made of a number of the hydrogen-substituted derivatives of the pesticide mirex, C 10 Cl 12 . Although a complete vibrational assignment was not possible owing to the large number of allowed transitions and the small number of observed bands, peak patterns based on substitution were determined and identifications made based on these trends. The spectral data provided should make it possible to identify the various hydrogen-substituted derivatives.

Induction of the hepatic cytochrome P-450 dependent monooxygenase system by cis- and tranas-5,10-dihydrogen mirex

The hepatic induction of cytochrome P-450-dependent monooxygenase components by cis- and trans-5,10-dihydrogen mirex was studied in male and female laboratory rats. There were sex-dependent differences between the two isomeric derivatives of mirex. The cis-isomer significantly increased aniline hydroxylase activity in the female, but not in the male. In contrast, aminopyrine N-demethylase was significantly increased by the cis-isomer in both sexes. The trans-isomer increased the hydroxylase and N-demethylase activities in both sexes. The cis-isomer induced NADPH-cytochrome c reductase in the female, and the trans-isomer did not. Both isomers induced hepatic reductase activity in the male rat. No sex-dependent differences in the hepatic induction of cytochrome P-450 were observed for either isomer.