Eduardo Perez-Campos Mayoral

Molecules in seminal plasma related to platelets in preeclampsia

OBJECTIVE Prostaglandins present in seminal fluid are actively involved in vascular and non-vascular smooth muscle maintenance, reproduction, and inflammatory processes. Seminal plasma contains molecules, such as oxylipins, which possess cell signaling functions. Several studies have shown that specific molecules in seminal fluid can increase passive diffusion, and cause interactions in the female reproductive tract. This may provoke a cascade of cellular and molecular changes in general health and certain diseases. This study examines the hypothesis that the molecules in seminal fluid are involved in platelet activity. The molecules diffuse through cells and membranes, affecting Hoxa 10, binding ganglioside pathways, and acting over platelet function. When these molecules are at low levels, they may trigger prothrombotic states, explaining the pathophysiology of haemostatic response, such as preeclampsia, and increased risk of cardiovascular disease.

Platelets and Platelet-Derived Microvesicles as Immune Effectors in Type 2 Diabetes

The association between type 2 diabetes mellitus (T2DM) and systemic inflammation may increase platelet reactivity and the accelerated development of vascular disease. Platelets are able to modulate the function of immune cells via the direct release of growth factors and pro-inflammatory chemokines through the production of microvesicles. The microvesicles trigger a transcellular delivery system of bioactive molecules to other cells acting as vectors in the exchange of biological information. Here, we consider the influence of platelets and platelet-derived microvesicles on cells of the immune system and the implications in the pathogenesis of T2DM.

Corrigendum to “Molecules in seminal plasma related to platelets in preeclampsia” [Med. Hypotheses 93 (2016) 27–29]

http://dx.doi.org/10.1016/j.mehy.2016.12.014 DOI of original article: http://dx.doi.org/10.1016/j.mehy.2016.05.009 ⇑ Corresponding author at: Unit of Biochemistry ITO-UNAM, Research Center, UNAM-UABJO-Medicine, Ex Hacienda de Aguilera S/N, Oaxaca, Oax, Mexico. E-mail address: perezcampos@prodigy.net.mx (E. Pérez-Campos). Iván Antonio García-Montalvo , Gabriel Mayoral Andrade , Laura Perez-Campos Mayoral , Socorro Pina Canseco , Ruth Martinez Cruz , Margarito Martínez-Cruz , Edgar Zenteno , Eduardo Perez-Campos Mayoral , Itandehui Gallegos Velasco , Maria Teresa Hernandez-Huerta , Diana Matias-Perez , Eduardo Pérez-Campos a,b,c,⇑

Reduced platelet aggregation in women after intercourse: a possible role for the cyclooxygenase pathway

We hypothesise that molecules in the cyclooxygenase pathway affect platelet activity when seminal fluid (SF) is present. We considered the influence of SF on platelet aggregation in women, and believe that the prostanoids in SF signalling are significant. Thirty‐one female subjects were studied, 20 of whom were sexually active. Male partners were given either aspirin or indomethacin to inhibit cyclooxygenase. The 6‐keto prostaglandin F1α (6‐keto PGF1α) and prostaglandin E metabolite (PGE‐M) in SF were measured by competitive assay. Platelets and prostanoids were evaluated in women, periodically, before and after intercourse. The platelets were tested with adenosine diphosphate (ADP) and arachidonic acid (AA). To block the interaction between the uterus and SF, some couples used condoms. We found that the 6‐keto prostaglandin F1α in urine at 2 hours post‐intercourse (1418.75 pg/mL, Std 688.39) was greater than pre‐intercourse (772.68 pg/mL, Std 116.54). Post‐intercourse, a transient decrease in platelet aggregation was observed in women whose partners did not use condoms. Averages for platelet aggregation were 20.16% with ADP, and more significantly, 37.79% with AA after 2 hours. In contrast, couples using condoms showed no changes, averaging 64.02% with ADP and 72.06% with AA. Women whose partners were taking aspirin or indomethacin also showed no changes. SF from men taking aspirin or indomethacin led to no reduction in platelet aggregometry in their partners. These results indicate that in cases of exposure to SF, the transient change in womens platelet activity could be related to the cyclooxygenase pathway.