Edwin T. Nishimura

Carrier State in Human Acatalasemia

The heterozygous carrier state of a rare hereditary disease, acatalasemia, has been defined biochemically. Affected homozygotes have no blood catalase activity, whereas heterozygotes show activities intermediate between this inactivity and the activity of normal controls, without overlap. Pedigrees show a high frequency of consanguineous marriages.

ISOZYMES OF HUMAN AND RAT CATALASES.

Abstract Catalases purified from livers of human and rats showed significant differences when compared to purified erythrocyte catalases of these species by immunoelectrophoresis and sucrose gradient centrifugation. Furthermore, rat hepatic catalase was fractionated into two components of high specific activities by chromatography through DEAE-celite-calcium phosphate gel column. These fractions designated, RHC-A and RHC-B, were shown to yield different immunoelectrophoretic patterns. Evidence for the subunit hypothesis of catalase is presented and discussed.

The biologic consequences of the immediate type hypersensitivity transferred from man to monkey

The model of human hypersensitivity using nonhuman primates passively sensitized by human allergic sera was investigated to evaluate certain physiologic, electrocardiographic, routine light and electron microscopic changes, and immunologic alterations. Varying types of conduction defects and ischemic electrocardiographic patterns occurred during anaphylaxis following a fall in systemic pressure and an occasional marked increase in pulmonary artery pressure. A series of immunologic studies demonstrated reactions which detected the presence of human antibody in the sensitized recipients.

Immunological evidence of repressed catalase synthesis in livers of tumor-bearing mice.

Abstract A quantitative immunochemical procedure for the determination of hepatic catalase in livers of mice is described. By employing this method, the data obtained suggest a sharp and marked diminution of synthesis of catalase in livers of mice with an advanced growth of Ehrlich ascites tumor. The magnitude of depression in the activity of liver catalase is related to the reduction in absolute concentration of the enzyme protein in liver homogenates of tumor-bearing animals.