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Dive into the research topics where Efstathia Ioannou is active.

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Featured researches published by Efstathia Ioannou.


Marine Biotechnology | 2010

Anti-microfouling activity of lipidic metabolites from the invasive brown alga Sargassum muticum (Yendo) fensholt.

Erwan Plouguerné; Efstathia Ioannou; Panagiota Georgantea; Constantinos Vagias; Vassilios Roussis; Claire Hellio; Edouard Kraffe; Valérie Stiger-Pouvreau

The purification of the chloroform extract from the brown invasive macroalga Sargassum muticum, through a series of chromatographic separations, yielded 12 fractions that were tested against strains of bacteria, microalgae, and fungi involved in marine biofilm formation. The chemical composition of four (a, c, g, and k) out of the six fractions that exhibited anti-microfouling activity was investigated. Fraction a contained saturated and unsaturated linear hydrocarbons (C12–C27). Arachidonic acid was identified as the major metabolite in fraction c whereas fraction g contained mainly palmitic, linolenic, and palmitoleic acids. Fraction k was submitted to further purification yielding the fraction kAcaF1e that was composed of galactoglycerolipids, active against the growth of two of the four bacterial strains (Shewanella putrefaciens and Polaribacter irgensii) and all tested fungi. These promising results, in particular the isolation and the activity of galactoglycerolipids, attest the potential of the huge biomass of S. muticum as a source of new environmentally friendly antifouling compounds.


Journal of Natural Products | 2010

Cytotoxic halogenated metabolites from the Brazilian red alga Laurencia catarinensis.

Cintia Lhullier; Miriam Falkenberg; Efstathia Ioannou; Antonio Quesada; Panagiota Papazafiri; Paulo Antunes Horta; Eloir Paulo Schenkel; Constantinos Vagias; Vassilios Roussis

Seven new (1-7) and seven previously reported (8-14) halogenated metabolites were isolated from the organic extract of the Brazilian red alga Laurencia catarinensis. The structure elucidation and the assignment of the relative configurations of the new natural products were based on detailed NMR and MS spectroscopic analyses, whereas the structure of metabolite 6 was confirmed by single-crystal X-ray diffraction analysis. The absolute configuration of metabolite 1 was determined using the modified Moshers method. The in vitro cytotoxicity of compounds 1-14 was evaluated against HT29, MCF7, and A431 cell lines.


Steroids | 2009

5α,8α-Epidioxysterols from the gorgonian Eunicella cavolini and the ascidian Trididemnum inarmatum: Isolation and evaluation of their antiproliferative activity

Efstathia Ioannou; Ayman F. Abdel-Razik; Maria Zervou; Dimitrios Christofidis; Xanthippi Alexi; Constantinos Vagias; Michael N. Alexis; Vassilios Roussis

Three new (1, 4, 9) and nine previously reported (2, 3, 5-8, 10-12) 5alpha,8alpha-epidioxysterols were isolated from the organic extracts of the gorgonian Eunicella cavolini and the ascidian Trididemnum inarmatum. The structures and relative configurations of 1-12 were established on the basis of detailed NMR spectroscopic analyses and comparison with the literature. The growth inhibitory effects of 1-12 were evaluated against MCF-7 human breast cancer cells. Compound 1, bearing a cyclopropyl moiety in the side chain, exhibited the highest antiproliferative activity.


Metabolic Engineering | 2015

Reconstructing the chemical diversity of labdane-type diterpene biosynthesis in yeast

Codruta Ignea; Efstathia Ioannou; Panagiota Georgantea; Sofia Loupassaki; Fotini A. Trikka; Angelos K. Kanellis; Antonios M. Makris; Vassilios Roussis; Sotirios C. Kampranis

Terpenes are a large class of natural products, many of which are used in cosmetics, pharmaceuticals, or biofuels. However, terpenes industrial application is frequently hindered by limited availability of natural sources or low yields of chemical synthesis. In this report, we developed a modular platform based on standardized and exchangeable parts to reproduce and potentially expand the diversity of terpene structures in Saccharomyces cerevisiae. By combining different module-specific parts, we exploited the substrate promiscuity of class I diterpene synthases to produce an array of labdane-type scaffolds. These were subsequently modified by a scaffold decoration module consisting of a mutant library of a promiscuous cytochrome P450 to afford a range of hydroxylated diterpenes. Further P450 protein engineering yielded dedicated and efficient catalysts for specific products. Terpenes produced include precursors of pharmacologically important compounds, molecules that are difficult to obtain from natural sources, or new natural products. The approach described here provides a platform on which additional gene mining, combinatorial biosynthesis, and protein engineering efforts can be integrated to sustainably explore the terpene chemical space.


New Phytologist | 2011

Role of lupeol synthase in Lotus japonicus nodule formation

Costas Delis; Afrodite Krokida; Sofia Georgiou; Luis M. Peña-Rodríguez; Nektarios Kavroulakis; Efstathia Ioannou; Vassilios Roussis; Anne Osbourn; Kalliope K. Papadopoulou

• Triterpenes are plant secondary metabolites, derived from the cyclization of 2,3-oxidosqualene by oxidosqualene cyclases (OSCs). Here, we investigated the role of lupeol synthase, encoded by OSC3, and its product, lupeol, in developing roots and nodules of the model legume Lotus japonicus. • The expression patterns of OSC3 in different developmental stages of uninfected roots and in roots infected with Mesorhizobium loti were determined. The tissue specificity of OSC3 expression was analysed by in situ hybridization. Functional analysis, in which transgenic L. japonicus roots silenced for OSC3 were generated, was performed. The absence of lupeol in the silenced plant lines was determined by GC-MS. • The expression of ENOD40, a marker gene for nodule primordia initiation, was increased significantly in the OSC3-silenced plant lines, suggesting that lupeol influences nodule formation. Silenced plants also showed a more rapid nodulation phenotype, consistent with this. Exogenous application of lupeol to M. loti-infected wild-type plants provided further evidence for a negative regulatory effect of lupeol on the expression of ENOD40. • The synthesis of lupeol in L. japonicus roots and nodules can be solely attributed to OSC3. Taken together, our data suggest a role for lupeol biosynthesis in nodule formation through the regulation of ENOD40 gene expression.


Metabolic Engineering | 2015

Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase

Codruta Ignea; Fotini A. Trikka; Alexandros Nikolaidis; Panagiota Georgantea; Efstathia Ioannou; Sofia Loupassaki; Panagiotis Kefalas; Angelos K. Kanellis; Vassilios Roussis; Antonios M. Makris; Sotirios C. Kampranis

Terpenes have numerous applications, ranging from pharmaceuticals to fragrances and biofuels. With increasing interest in producing terpenes sustainably and economically, there has been significant progress in recent years in developing methods for their production in microorganisms. In Saccharomyces cerevisiae, production of the 20-carbon diterpenes has so far proven to be significantly less efficient than production of their 15-carbon sesquiterpene counterparts. In this report, we identify the modular structure of geranylgeranyl diphosphate synthesis in yeast to be a major limitation in diterpene yields, and we engineer the yeast farnesyl diphosphate synthase Erg20p to produce geranylgeranyl diphosphate. Using a combination of protein and genetic engineering, we achieve significant improvements in the production of sclareol and several other isoprenoids, including cis-abienol, abietadiene and β-carotene. We also report the development of yeast strains carrying the engineered Erg20p, which support efficient isoprenoid production and can be used as a dedicated chassis for diterpene production or biosynthetic pathway elucidation. The design developed here can be applied to the production of any GGPP-derived isoprenoid and is compatible with other yeast terpene production platforms.


Journal of Natural Products | 2011

Dolabellanes with antibacterial activity from the brown alga Dilophus spiralis.

Efstathia Ioannou; Antonio Quesada; M. Mukhlesur Rahman; Simon Gibbons; Constantinos Vagias; Roussis

Seventeen diterpenes featuring the dolabellane skeleton (1-17) were isolated from the organic extracts of the brown alga Dilophus spiralis. Seven compounds are new natural products (1, 3, 5, 6, 11, 14, 15) and eight are structurally revised (2, 4, 7-10, 12, 13), among which three are reported for the first time from a natural source (4, 9, 10). The structure elucidation and the assignment of the relative configurations of the isolated natural products were based on detailed analyses of their spectroscopic data. The structure of metabolite 10 was confirmed by single-crystal X-ray diffraction analysis, whereas the absolute configurations of compounds 2, 4-10, 12, and 13 were determined using the modified Moshers method on the semisynthetic product 18 and chemical interconversions. The antibacterial activities of compounds 1-18 were evaluated against six strains of Staphylococcus aureus, including multidrug- and methicillin-resistant variants.


Proceedings of the National Academy of Sciences of the United States of America | 2016

Carnosic acid biosynthesis elucidated by a synthetic biology platform

Codruta Ignea; Anastasia Athanasakoglou; Efstathia Ioannou; Panagiota Georgantea; Fotini A. Trikka; Sofia Loupassaki; Vassilios Roussis; Antonios M. Makris; Sotirios C. Kampranis

Significance Here we report on the development of a modular terpene production platform as a screening tool for the functional characterization of next-generation sequencing information and on the application of this system in the characterization of the biosynthetic pathway leading to the potent antioxidant carnosic acid in Lamiaceae. In addition to revealing an important plant natural product biosynthetic pathway, this work highlights the usefulness of synthetic biology approaches in the elucidation of plant natural product biosynthetic pathways and develops yeast as a tool to exploit the wealth of next-generation sequencing data that are constantly being produced through transcriptomic, genomic, or metagenomic studies. The approach described here will be useful to numerous researchers studying terpene biosynthesis in plants and microorganisms. Synthetic biology approaches achieving the reconstruction of specific plant natural product biosynthetic pathways in dedicated microbial “chassis” have provided access to important industrial compounds (e.g., artemisinin, resveratrol, vanillin). However, the potential of such production systems to facilitate elucidation of plant biosynthetic pathways has been underexplored. Here we report on the application of a modular terpene production platform in the characterization of the biosynthetic pathway leading to the potent antioxidant carnosic acid and related diterpenes in Salvia pomifera and Rosmarinus officinalis. Four cytochrome P450 enzymes are identified (CYP76AH24, CYP71BE52, CYP76AK6, and CYP76AK8), the combined activities of which account for all of the oxidation events leading to the biosynthesis of the major diterpenes produced in these plants. This approach develops yeast as an efficient tool to harness the biotechnological potential of the numerous sequencing datasets that are increasingly becoming available through transcriptomic or genomic studies.


Bioorganic & Medicinal Chemistry | 2009

9,11-Secosterols with antiproliferative activity from the gorgonian Eunicella cavolini

Efstathia Ioannou; Ayman F. Abdel-Razik; Xanthippi Alexi; Constantinos Vagias; Michael N. Alexis; Vassilios Roussis

Four new 9,11-secosterols (2, 4-6), along with two previously reported ones (1, 3) were isolated from the organic extract of the gorgonian Eunicella cavolini. The structures and relative configurations of the isolated natural products were established on the basis of detailed NMR spectroscopic analysis. Metabolites 1 and 2 were found to strongly inhibit the growth of LNCaP human prostate adenocarcinoma cells and the estrogen-dependent growth of MCF-7 human breast adenocarcinoma cells.


BMC Genomics | 2015

Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes.

Fotini A. Trikka; Alexandros Nikolaidis; Codruta Ignea; Aphrodite Tsaballa; Leto-Aikaterini Tziveleka; Efstathia Ioannou; Vassilios Roussis; Eleni A. Stea; Dragana Božić; Anagnostis Argiriou; Angelos K. Kanellis; Sotirios C. Kampranis; Antonios M. Makris

BackgroundSalvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems.ResultsIn the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera.ConclusionsOur work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites.

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Vassilios Roussis

National and Kapodistrian University of Athens

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Constantinos Vagias

National and Kapodistrian University of Athens

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Panagiota Georgantea

National and Kapodistrian University of Athens

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Panagiotis Kefalas

Mediterranean Agronomic Institute of Chania

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Sofia Loupassaki

Mediterranean Agronomic Institute of Chania

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Olga Tzakou

National and Kapodistrian University of Athens

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Stefanos Kikionis

National and Kapodistrian University of Athens

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Antonios M. Makris

Mediterranean Agronomic Institute of Chania

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