Eiichi Miyagawa

CELLULAR FATTY ACID COMPOSITION IN BACTEROIDES ORALIS AND BACTEROIDES RUMINICOLA

Cellular fatty acid composition of 9 strains of Bacteroides ruminicola, 16 strains of B. oralis, and 6 reference strains including B. splachnicus, B. melaninogenicus var. melaninogenicus, B. melaninogenicus var. intermedius, B. asaccharolyticus, B. fragilis, and B. vulgatus was determined by gas-liquid chromatography. The organisms tested were divided into three major groups based on composition.In the first group, 6 strains of B. ruminicola and 11 strains of B. oralis, B. melaninogenicus var. melaninogenicus, B. melaninogenicus var. intermedius, B. fragilis, and B. vulgatus were included. Anteiso-15:0 was the most abundant acid, and iso-15:0 and/or 3-OH-iso-17:0 were present in large quantity in most strains of this group. In the second group, 2 strains of B. ruminicola, and 1 strain of B. oralis, B. splachnicus, and B. asaccharolyticus were included. Iso-15:0 was the most abundant acid, and other major acids present were anteiso-15:0, 3-OH-iso-17:0 and so on. In the third group, 1 strain of B. ruminicola and 4 strains of B. oralis were included. A rather large amount of 16:0 was present, and other major acids found were anteiso-15:0 and/or iso-15:0.

Investigations into the location of Treponema hyodysenteriae in the cecum of experimentally infected young broiler chicks by light- and electronmicroscopy

Numerous Treponema hyodysenteriae were present both on the mucosal surface and in the deep crypts of the cecum of young broiler chicks 7 and 14 days after inoculation with the treponemes. The treponemes in the ceca of chicks inoculated with 10(8) cells were observed more frequently than those of chicks inoculated with 10(7) cells. The treponemes in the ceca were observed by light microscopy, scanning electron microscopy and transmission electron microscopy. The lesions were primarily confined to the cecum. Desquamation of epithelial cells, edema, leukocytic infiltration and hemorrhage were observed in the mucosae.