Eiichi Sakuraba

Nd:YAG laser irradiation of the human dental pulp: implications as a predictor of pulp hemodynamics.

The aim of study this was to evaluate in vivo the pulpal blood flow rate, pulpal responsiveness, systemic blood pressure, and pulse rate during Nd:YAG laser irradiation of an isolated tooth.

Reversible suppression of action potentials of Xenopus tactile nerve fibers to Nd:YAG laser irradiation with and without Chinese ink

Abstract We have reported that pain could be effectively relieved by Nd:YAG laser irradiation in dental or periodontal treatment, and that painting Chinese ink on the mucogingival surface reinforces the efficacy of anesthesia with laser irradiation. However, the effect of Chinese ink on nerve action potentials (a.p.) in this pain relief is not clear. In this study Xenopus tactile nerve fibers were used to clarify this efficacy. Dorsal surface skin was removed with fine tactile nerve bundles, and was put on the filter paper absorbed physiological buffer solution, and fastened on the rubber mat. From a bundle, a.p. with tactile stimuli on the skin surface were recorded by a silver electrode, and counted by the spike counter. After noncontact Nd:YAG laser irradiation (15 pps, 100 mJ, 60 s), with or without Chinese ink painting, tactile responses were periodically recorded. Results were as follows: (1) The conduction of a.p. was reversibly suppressed in the bundle; (2) Chinese ink painting has a considerable effect on reversible suppression; and (3) the suppressive effect was not certain in nerve bundles without Chinese ink painting. These results endorse that Chinese ink painting is effective for patients on dental or periodontal pain control using laser irradiation.

Effects of the Nd:YAG laser on the pain associated with tooth movement

Abstract The purpose of this study was to evaluate the effects of Nd:YAG laser irradiation on the pain associated with orthodontic treatment. Two subjects participated in this study with their informed consent to the Nd:YAG laser irradiation test protocol. Case 1: The patient is a 27-year-old male who was worn maxillary lateral expansion appliance. Case 2: The patient is a 22-year-old female with placement of a full edgewise appliance. The gingival areas were coated with Chinese ink and were immediately irradiated with a Nd:YAG laser (Pulse Master, American Dental Technology, Christi, TX) for 30 s at 100 mJ/pulse and 15 pps. After a 30-s interval, the irradiation was repeated once more. The severity of pain was assessed by Visual Analog Scale (VAS) for 1 day except sleeping time. In each case, pain relief effects were observed immediately after the irradiation. The effect lasted for 9 h in Case 1 and 12 h in Case 2. This study suggests that the Nd:YAG laser irradiation would be useful for pain relief in orthodontic treatment.

Pain relief effects of semiconductor laser irradiation

Abstract The purpose of this study was to evaluate the pain relief effects of continuous wave and chopper pulsed wave by semiconductor laser irradiation. The irradiation conditions were as follows: Group A, continuous wave (laser energy 1 w, irradiation time 60 s); Group B, chopper pulsed wave of 20 ms (laser energy 2 w, irradiation time 60 s); Group C, chopper pulsed wave of 20 ms (laser energy 1 w, irradiation time 120 s); and Group D, dummy irradiation. The pain relief effects were evaluated on pulp thermal sensitivity and surface anaesthetic effect. (1) Evaluation of pulp thermal sensitivity: the degree of pulp thermal sensitivity was measured by a pain thermometer on irradiation sites and control sites. (2) Evaluation of surface anaesthetic effects: the mucobaccal holds were penetrated with a 30-gauge cartridge needle, and each subject reported an immediate response to needle penetration on irradiation sites and control sites. For these results of pain relief effects on pulp thermal sensitivity and surface anaesthetic effects, there was statistically significant difference in pain relief effects between the irradiation conditions of chopper pulsed wave and control (P

A Histological Study on Hybrid Materials Compounded with Porous Hydroxyapatite and Periodontal Ligament Cells in vivo.

We produced a hybrid material compounded with periodontal ligament cells, which may differentiate into osteoblasts and/or cementblasts, and porous hydroxyapatite granules. Hybrid materials were implanted in the jaws of rabbits from which periodontal ligament cells has been obtained. Periodontal ligament cells were obtained from the incisor roots of the rabbits and cultured at 37•Ž in ƒ¿ -MEM supplemented with 10% fetal calf serum and