Eisaku Fujimoto

Beneficial effect of basic fibroblast growth factor on the repair of full-thickness defects in rabbit articular cartilage

Abstract The effects of exogenous basic fibroblast growth factor (bFGF) on the repair of full-thickness cartilage defects were examined. Four-millimeter diameter, cylindrical defects were made in rabbit articular cartilage and were filled with human recombinant bFGF. The addition of bFGF to the defect induced the formation of a thick cartilage layer composed of chondrocytes and a metachromatic-stained matrix after 6 weeks. The score of the bFGF-treated tissue, as evaluated by a semiquantitative histological scale, was significantly higher than that of the untreated tissue. At 24 weeks, the cartilage-like matrix that contained the proteoglycans and type II collagen was thicker in the bFGF-treated tissue than in the untreated tissue. Immunohistochemical analysis of the tissues at 6–12 weeks with an anti-bFGF monoclonal antibody suggested that a single application of bFGF increased the number of differentiating chondrocytes that synthesized bFGF at a high level. In contrast, immunostaining of the tissues at 6–12 weeks with a monoclonal antibody against proliferating cell nuclear antigen showed that the number of proliferating cells in the bFGF-treated tissue was fewer than in the untreated tissue. These findings suggest that administration of bFGF into cartilagenous defects promotes the differentiation of chondrocytes and their matrix synthesis, and that this growth factor is useful for improving cartilage repair.

Mr Evaluation of Human Anterior Cruciate Ligament Autograft on Oblique Axial Imaging

PURPOSE The purpose was to observe the changing MR appearance of stable anterior cruciate ligament (ACL) grafts on oblique axial images. METHOD Fifty-five knees in 44 patients were studied with MRI 1-54 months after arthroscopic ACL reconstruction with double-looped autogenous semitendinosus and gracilis tendons. Knees with poor stability were excluded from this study. Examinations were performed at 0.2 T with SE proton density and T2-weighted sagittal and oblique axial images. RESULTS High signal intensity areas covered the grafts 1-3 months after surgery. The high signal subsequently extended into the intertendinous bundles. The entire graft gradually became a low signal intensity bundle again after 12 months. Grafts were classified by their appearance on the oblique axial images. CONCLUSION We conclude that high signal intensity can be seen within stable ACL grafts.

Significant effect of the posterior tibial slope and medial/lateral ligament balance on knee flexion in total knee arthroplasty.

PurposeThe intra-operative femorotibial joint gap and ligament balance, the predictors affecting these gaps and their balances, as well as the postoperative knee flexion, were examined. These factors were assessed radiographically after a posterior cruciate-retaining total knee arthroplasty (TKA). The posterior condylar offset and posterior tibial slope have been reported as the most important intra-operative factors affecting cruciate-retaining-type TKAs. The joint gap and balance have not been investigated in assessments of the posterior condylar offset and the posterior tibial slope.MethodsThe femorotibial gap and medial/lateral ligament balance were measured with an offset-type tensor. The femorotibial gaps were measured at 0°, 45°, 90° and 135° of knee flexion, and various gap changes were calculated at 0°–90° and 0°–135°. Cruciate-retaining-type arthroplasties were performed in 98 knees with varus osteoarthritis.ResultsThe 0°–90° femorotibial gap change was strongly affected by the posterior condylar offset value (postoperative posterior condylar offset subtracted by the preoperative posterior condylar offset). The 0°–135° femorotibial gap change was significantly correlated with the posterior tibial slope and the 135° medial/lateral ligament balance. The postoperative flexion angle was positively correlated with the preoperative flexion angle, γ angle and the posterior tibial slope. Multiple-regression analysis demonstrated that the preoperative flexion angle, γ angle, posterior tibial slope and 90° medial/lateral ligament balance were significant independent factors for the postoperative knee flexion angle. The flexion angle change (postoperative flexion angle subtracted by the preoperative flexion angle) was also strongly correlated with the preoperative flexion angle, posterior tibial slope and 90° medial/lateral ligament balance.ConclusionThe postoperative flexion angle is affected by multiple factors, especially in cruciate-retaining-type TKAs. However, it is important to pay attention not only to the posterior tibial slope, but also to the flexion medial/lateral ligament balance during surgery. A cruciate-retaining-type TKA has the potential to achieve both stability and a wide range of motion and to improve the patients’ activities of daily living.

Pretibial cyst formation after anterior cruciate ligament reconstruction using auto hamstring grafts: two case reports in a prospective study of 89 cases

Eighty-nine cases after anterior cruciate ligaments (ACL) reconstruction were followed prospectively with magnetic resonance imaging (MRI). The patients were examined using axial and sagittal MRI at least twice during the postoperative evaluation of reconstructed ACL. Two cases of pretibial cyst formation were observed. At the time of cyst formation, neither patient had any subjective or objective evidence of knee instability. The cyst of one case communicated with the intra-articular. The minimum follow-up period after the surgical excision was 9 months, with no evidence of recurrence. We might speculate that the critical period for cyst formation in both patients occurred at less than 12 months after their ACL reconstruction. We concluded that the cyst formation was most likely due to incomplete graft tendon incorporation within the osseous tunnel.

Appearance of anterior cruciate ligament autografts in their tibial bone tunnels on oblique axial MRI.

The objective of this study was to observe the changing appearance of human anterior cruciate ligament (ACL) grafts in their tibial bone tunnels by MRI using oblique axial images. One-hundred and eight knees in 75 patients were studied by MRI at 1-33 months after arthroscopic ACL reconstructions using double-looped, autogenous semitendinosus and/or gracilis tendons. Knees with poor stability were excluded from this study. The examinations were performed at 0.2T with spin echo proton density and T2-weighted oblique axial images. Appearances of grafts were mainly described on spin echo proton density images based upon time after surgery. The grafts appeared as homogeneous, low signal intensity areas in the bone tunnels at 1 month after the surgery. Ring-shaped low signal intensity areas were observed along the wall of the bone tunnels in the 2- to 3-month group. In many grafts from this group, each tendinous bundle appeared as a low signal area separated by a high signal intensity area. In all cases in the 4- to 6-month group, the thickness of the ring-shaped low signal intensity area had increased, whereas the thickness of the high signal intensity area had decreased. In almost all of the cases, the interior of the bone tunnel gradually became a homologous low signal intensity region by 7 to 12 months after the surgery. According to these results, it is suggested that the maturation of the tendon-bone interface was completed from 6 to 12 months after the ACL reconstruction.

Significant effect of the posterior tibial slope on the weight-bearing, midflexion in vivo kinematics after cruciate-retaining total knee arthroplasty.

The purpose of the present study was to compare weight bearing (WB) and non-WB conditions, and to evaluate the effect of the posterior tibial slope (PTS) on the in vivo kinematics of 21 knees after posterior cruciate ligament-retaining total knee arthroplasty during midflexion using 2-dimensional/3-dimensional registration. During WB, medial pivot and bicondylar rollback were observed. During non-WB, both the medial and lateral condyles moved significantly more anteriorly as compared to the WB state. These patients were divided into 2 groups according to their PTS. The large PTS group showed a significant posterior displacement of the medial femoral condyle as compared with the small PTS group, but no significant difference was observed at the lateral femoral condyle during both WB and non-WB. The PTS influenced knee kinematics through gravity (124/125).

Technical Considerations and Accuracy Improvement of Accelerometer-Based Portable Computer Navigation for Performing Distal Femoral Resection in Total Knee Arthroplasty

BACKGROUND Accelerometer-based computer navigation has been shown to be highly accurate for performing distal femoral and proximal tibial component alignment in total knee arthroplasty (TKA), although the procedure for the femoral component is less accurate than for the tibial component. METHODS First, 30 knees without hip osteoarthritis or proximal femoral surgeries were selected. Sequential hip adduction, abduction, and flexion were performed, and the femoral head was monitored fluoroscopically in the coronal plane before TKA. Significantly more movement was detected during hip adduction than during abduction and flexion. Then, postoperative femoral and tibial component alignment was retrospectively evaluated in 48 TKAs before fluoroscopic monitoring (early group) and in the next 61 TKAs with femoral registration using smaller adduction movements to avoid large femoral head movements (later group). Another 47 TKAs treated with the conventional intramedullary method for the distal femoral component and the extramedullary method for the proximal tibial component were also analyzed (IM and EM group) for historic control. RESULTS Significantly large variances in the femoral component implantation of the early group were detected in both the coronal and sagittal planes. The sagittal femoral implantation angle of the early group (4.6 ± 3.0°) was significantly larger than that of the later group (3.2 ± 1.8°) when 3.5° was the target for both groups. No significant difference was detected in the variances of either the coronal or sagittal tibial component implantation, although the coronal tibial implantation angle was significantly smaller (-1.3 ± 1.3°valgus) in the early group than in the other groups. CONCLUSION Accelerometer-based navigation sometimes has technical issues during registration associated with hip adduction. We showed that femoral registration without large adduction movements will enable more accurate femoral implantation. Surgeons should also keep in mind that the coronal tibial component is likely to be in valgus alignment (about 1°) even if a neutral angle (0°) is selected with this particular device.

An early return to vigorous activity may destabilize anterior cruciate ligaments reconstructed with hamstring grafts 1 1No commercial party having a direct financial interest in the results of the research supporting this article has or will confer a benefit upon the authors or upon any organization with which the authors are associated.

OBJECTIVE To evaluate the actual date of the return to activity and its impact on the postsurgical stability of anterior cruciate ligament reconstruction (ACLR) using hamstring grafts. DESIGN A retrospective analysis. The time of return to activity was determined by a questionnaire at 24 to 36 months after ACLRs. SETTING An orthopedic center. PARTICIPANTS Fifty consecutive patients who had ACLRs using hamstring grafts 24 to 36 months earlier. INTERVENTIONS Not applicable. Main outcome measure The time of return to activity from a questionnaire and serial KT-2000 data at 3, 6, 12, 18, and 24 months postsurgery. RESULTS Patients were divided into 2 groups according to their KT-2000 side-to-side difference at 12, 18, and 24 months postsurgery. Group I consisted of patients whose differences were 3mm or less. Group II consisted of patients whose differences were more than 3mm. At 12 and 18 months postsurgery, significant differences were detected for the time of return to running and full-speed running. A multiple regression analysis for postsurgical stability at 24 months and the time of return to these 5 activities indicated that the time of return to full-speed running and sports activities had an effect on ACL stability. CONCLUSIONS An early return to vigorous activities is not recommended in patients undergoing ACLRs with hamstring grafts.