Eiso Hiyama

Immunohistochemical analysis of N-myc protein expression in neuroblastoma: Correlation with prognosis of patients

We have analyzed N-myc gene amplification and N-myc protein expression in 41 primary neuroblastomas. In this series, 22 patients are currently alive and disease-free, whereas 19 patients have died or are alive with progressive disease. All tumor samples were obtained at operation. N-myc gene amplification was detected by Southern blot analysis, and N-myc protein expression was detected using Bouin-fixed, paraffin-embedded tissue sections and immunohistochemical staining with anti-N-myc gene products serum. N-myc protein expression was detected in all 9 tumors with N-myc gene amplification (greater than or equal to 10 N-myc gene copies). Among 19 patients with poor prognosis, N-myc gene amplification was detected in 8 (42%) and N-myc protein expression in 18 (95%); neither was detected in 20 of the 22 patients who survived free of disease. We conclude that the immunohistochemical detection of N-myc protein expression is one of the most unfavorable prognostic factors in neuroblastoma patients.

Studies of the Intraperitoneal Antibiotic Administration.

抗菌剤腹腔内1回投与が全身投与よりも術後感染予防に有用であるかを検討した. 消化器手術32例を対象にセフェム剤 (3剤) を1g点滴静注 (DI), 腹腔内投与 (IP) し体内動態を測定した. IP群での腹水中濃度は投与後30分, 1時間で1, 000,300μg/mlとDI群より有意に高い値が得られた. しかし, 投与後4, 6時間ではDI群と有意差のない値 (4-2μg/ml) となり, DI群に比べ有意の有効濃度の持続時間の廷長は得られなかった. 一方, flomoxef sodium (FMOX) は大腸菌に対し400, 40μg/mlでおのおの2, 4時間野接触時間で良好な殺菌効果を認めたが, 4μg/ml, 6時間接触では殺菌効果は得られなかった.緑膿菌, メチシリン耐性ブドウ球菌ではFMOX 400μg/ml, 6時間接触時間でも殺菌作用は得られなかった.高濃度の持続時間で期待できないので抗菌剤腹腔内1回投与では, 上記細菌.対象とした術後感染予防において全身投与以上の有用性は得られないと考えられた.