Elena Díaz-Almela

Effects of fish farm waste on Posidonia oceanica meadows: Synthesis and provision of monitoring and management tools

This paper provides a synthesis of the EU project MedVeg addressing the fate of nutrients released from fish farming in the Mediterranean with particular focus on the endemic seagrass Posidonia oceanica habitat. The objectives were to identify the main drivers of seagrass decline linked to fish farming and to provide sensitive indicators of environmental change, which can be used for monitoring purposes. The sedimentation of waste particles in the farm vicinities emerges as the main driver of benthic deterioration, such as accumulation of organic matter, sediment anoxia as well as seagrass decline. The effects of fish farming on P. oceanica meadows are diverse and complex and detected through various metrics and indicators. A safety distance of 400 m is suggested for management of P. oceanica near fish farms followed by establishment of permanent seagrass plots revisited annually for monitoring the health of the meadows.

Implications of Extreme Life Span in Clonal Organisms: Millenary Clones in Meadows of the Threatened Seagrass Posidonia oceanica

The maximum size and age that clonal organisms can reach remains poorly known, although we do know that the largest natural clones can extend over hundreds or thousands of metres and potentially live for centuries. We made a review of findings to date, which reveal that the maximum clone age and size estimates reported in the literature are typically limited by the scale of sampling, and may grossly underestimate the maximum age and size of clonal organisms. A case study presented here shows the occurrence of clones of slow-growing marine angiosperm Posidonia oceanica at spatial scales ranging from metres to hundreds of kilometres, using microsatellites on 1544 sampling units from a total of 40 locations across the Mediterranean Sea. This analysis revealed the presence, with a prevalence of 3.5 to 8.9%, of very large clones spreading over one to several (up to 15) kilometres at the different locations. Using estimates from field studies and models of the clonal growth of P. oceanica, we estimated these large clones to be hundreds to thousands of years old, suggesting the evolution of general purpose genotypes with large phenotypic plasticity in this species. These results, obtained combining genetics, demography and model-based calculations, question present knowledge and understanding of the spreading capacity and life span of plant clones. These findings call for further research on these life history traits associated with clonality, considering their possible ecological and evolutionary implications.

Direct Evidence of Imbalanced Seagrass (Posidonia oceanica) Shoot Population Dynamics in the Spanish Mediterranean

Direct census of shoots tagged in permanent plots was used to assess the present (2000–2002)Posidonia oceanica population dynamics in 25 meadows along the Spanish Mediterranean Coast. Shoot density ranged from 154±8 to 1,551±454 shoots m−2, absolute shoot mortality from 5±0 to 249±53 shoots m−2 yr−1, and absolute shoot recruitment from <5 ±1 to 62±42 shoots m−2yr−1. Specific shoot mortality and recruitment rates, which are mathematically and statistically (p>0.05) independent of shoot density, varied from 0.015±0.006 to 0.282±0.138 yr−1 and 0.018±0.005 to 0.302±0.093 yr−1, respectively. Absolute shoot mortality rate was scaled to shoot density (Pearson correlation, r=0.78, p<0.0001), and variability in specific shoot recruitment rate was partially due to differences in the percentage of growing apexes, which produce most of the recruits within the population (Pearson correlation, r=0.50, p<0.001), demonstrating the existence of structural constraints on shoot demography. Shoot half-life was estimated to range from 2.5 to 60.4 yr and meadow turnover times between 6.7 yr and more than a century, provided current estimates of shoot mortality, recruitment rates, and density remain uniform. There were differences in shoot mortality and recruitment at the regional scale, with the meadows developing along the coast of the Spanish mainland experiencing the highest shoot mortality (Tukey test, p<0.05) and tending to exhibit the highest shoot recruitment. The low shoot recruitment did not balance shoot mortality in most (60%) of the meadows, showing a prevalence of declining populations among the 25 meadows studied (Wilcoxon ranked sign test, p<0.0005). This study demonstrates the power of direct census of seagrass shoots in permanent plots to evaluate the present status of seagrass meadows, to detect on-going population decline, and to provide some insight onto the possible factors involved. The incorporation of direct census of seagrass meadows to monitoring programs will help provide the early-warning signals necessary to support management decisions to conserve seagrass meadows.

Benthic input rates predict seagrass (Posidonia oceanica) fish farm-induced decline

Fish farms represent a growing source of anthropogenic disturbance to benthic communities, and efficient predictors of such impacts are urgently needed. We explored the effects of fish farm benthic organic and nutrient inputs on the population dynamics of a key seagrass species (Posidonia oceanica) in four Mediterranean deep meadows adjacent to sea bream and sea bass farms. We performed two annual plant censuses on permanent plots at increasing distance from farms and measured benthic sedimentation rates around plots. High shoot mortality rates were recorded near the cages, up to 20 times greater than at control sites. Recruitment rates increased in variability but could not compensate mortality, leading to rapid seagrass decline within the first 100 m from cages. Seagrass mortality increased with total sedimentation rates (K=0.55, p<0.0002), and with organic matter (K=0.50, p=0.001), total nitrogen (K=0.46, p=0.002) and total phosphorus (K=0.56, p<3.10(-5)) inputs. P. oceanica decline accelerated above a phosphorus loading threshold of 50mg m(-2)day(-1). Phosphorus benthic sedimentation rate seems a powerful predictor of seagrass mortality from fish farming. Coupling direct measurements of benthic sedimentation rates with dynamics of key benthic species is proposed as an efficient strategy to predict fish farm impacts to benthic communities.