Elena M. Tul'skaya

1,3-poly(glycerol phosphate) chains in the cell wall of Streptomyces rutgersensis var. castelarense VKM Ac-238

The cell wall of Streptomyces rutgersensis var. castelarense contains structurally different chains of 1,3-type glycerol teichoic acid. Part of the molecules consisting of 20-25 monomers, carry on every third glycerol phosphate unit (at C-2) alpha-glucosamine residues, only half of which are N-acetylated. There are chains with O-lysine groups, and free nonsubstituted ones. The chain structure has been ascertained by chemical analysis and 13C- and 1H-NMR spectroscopy.

Cell wall anionic polymers of Streptomyces sp. MB-8, the causative agent of potato scab

The cell wall of Streptomyces sp. MB-8 contains a major teichoic acid, viz., 1,3-poly(glycerol phosphate) substituted with N-acetyl-alpha-D-glucosamine (the degree of substitution is 60%), a minor teichoic acid, viz., non-substituted poly(glycerol phosphate), and a family of Kdn (3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid)-containing oligomers of the following general structure: [carbohydrate structure: see text]. The composition of the oligomers was established using MALDI-TOF mass spectroscopy. The present study provides the second example of the identification of Kdn as a component of cell wall polymers of streptomycetes, which are the causative agents of potato scab.

A new structural type of teichoic acid and some chemotaxonomic criteria of two species Nocardiopsis dassonvillei and Nocardiopsis antarcticus.

AbstractThe cell-wall teichoic acids of Nocardiopsis dassonvillei IMRU 509T, IMRU 504 and IMRU 1250 and Nocardiopsis antarctius VKM Ac-836T have the same unique structure that has not heretofore been found in bacteria. The polymer is built of 10 to 13 repeating units:

Cell Wall Teichoic Acids in the Taxonomy and Characterization of Gram-positive Bacteria†

- 1snGro3 - P - 3 - \beta D - Ga1Nac1 - 2snGro3 - P -

Erythritolteichoic acid in the cell wall of Glycomyces tenuis VKM Ac‐1250

The structure has been established by chemical analysis and with NMR spectroscopy. A study of the cell-wall monosaccharide composition, peptidoglycan, and cell phospholipids also provides evidence for the close similarity of these two species.

Species specificity of teichoic acids in the actinomycete genus Glycomyces

Cell walls of the three Nocardiopsis albus subsp. albus strains, DSM 43377T, 43378 and 43120 contain structurally identical teichoic acids. The cell wall of each strain has three distinct types of teichoic acids: (1) unsubstituted 3,5-poly(ribitol phosphate), (2) 1,3-poly(glycerol phosphate) partially substituted at C-2 with α-N-acetylglucosamine residues, and (3) 1,5-poly(ribitol phosphate) with each ribitol unit carrying a 2,4-pyruvate ketal group. Types 1 and 3 are reported in prokaryotes for the first time. The structure of the teichoic acids was elucidated by chemical analysis and NMR-spectroscopic methods. Structural identity of the teichoic acids from the three strains belonging to the same species may demonstrate the species-specificity of these polymers.

Cell wall glycopolymers of Streptomyces albus, Streptomyces albidoflavus and Streptomyces pathocidini

Abstract The use of cell wall teichoic acids as chemotaxonomic markers in the systematics of members of the order Actinomycetales and the genus Bacillus is discussed. Numerous examples show that these polymers are species-specific for representatives of the genera Actinomadura, Agromyces, Brevibacterium, Glycomyces, Nocardioides, Nocardiopsis, Nonomuraea, Streptomyces, and type strains of the B. subtilis group. The structural diversity and abundance of such polymers in Gram-positive bacteria is demonstrated. Methods for the isolation of cell wall teichoic acids and their component analysis as well as chemical and NMR spectroscopic methods for the complete structure elucidation are described.

Pyruvylated cell wall glycopolymers of Promicromonospora citrea VKM A≿-665T and Promicromonospora sp. VKM A≿-1028

The structures of cell wall anionic carbohydrate-containing polymers in Streptomyces melanosporofaciens VKM Ac-1864T and phylogenetically close organisms—S. hygroscopicus subsp. hygroscopicus VKM Ac-831T, S. violaceusniger VKM Ac-583T, S. endus VKM Ac-1331T, S. endus VKM Ac-129, and S. rutgersensis subsp. castelarensis VKM Ac-832T—have been comparatively studied by chemical and NMR spectroscopic methods. The natural polymer of a new, previously unknown structure, Kdn (3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid) with β-galactose residues at C-9, has been found in the cell walls of all the strains under study. The cell walls of all the studied organisms contain three teichoic acids (TA): a predominant TA (1,3-poly(glycerol phosphate) with N-acetylated α-glucosaminyl substitutes by C-2 of glycerol, and minor TAs, 1,3-and 2,3-poly(glycerol phosphate) polymers without substitution. Their chains have O-acetyl and O-lysyl groups. Microorganisms of the above-mentioned species differ in the number of α-glucosaminyl substitutes and in the degree of their acetylation in the predominant teichoic acid.