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Dive into the research topics where Eliana Monteforte Cassaro Villalobos is active.

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Featured researches published by Eliana Monteforte Cassaro Villalobos.


Journal of Comparative Pathology | 2012

Equid herpesvirus type-1 exhibits neurotropism and neurovirulence in a mouse model.

Claudia Madalena Cabrera Mori; Enio Mori; L. L. Favaro; Caio Rodrigues dos Santos; Maria do Carmo Custódio de Souza Hunold Lara; Eliana Monteforte Cassaro Villalobos; Elenice Maria Siquetin Cunha; Paulo Eduardo Brandão; Leonardo José Richtzenhain; Paulo César Maiorka

Intranasal inoculation of equid herpesvirus type-1 (EHV-1) Brazilian strains A4/72 and A9/92 induced an acute and lethal infection in four different inbred mouse strains. Clinical and neurological signs appeared between the 2nd and 3rd day post inoculation (dpi) and included weight loss, ruffled fur, a hunched posture, crouching in corners, nasal and ocular discharges, dyspnoea, dehydration and increased salivation. These signs were followed by increased reactivity to external stimulation, seizures, recumbency and death. The virus was recovered consistently from the brain and viscera of all mice with neurological signs. Histopathological changes consisted of leptomeningitis, focal haemorrhage, ventriculitis, neuronal degeneration and necrosis, neuronophagia, non-suppurative inflammation, multifocal gliosis and perivascular infiltration of polymorphonuclear and mononuclear cells. Immunohistochemical examination demonstrated that EHV-1 strains A4/72 and A9/92 replicated in neurons of the olfactory bulb, the cortex and the hippocampus. In contrast, mice inoculated with the EHV-1 Brazilian strain A3/97 showed neither weight loss nor apparent clinical or neurological signs; however, the virus was recovered consistently from their lungs at 3 dpi. These three EHV-1 strains showed distinct degrees of virulence and tissue tropism in mice. EHV-1 strains A4/72 and A9/92 exhibited a high degree of central nervous system tropism with neuroinvasion and neurovirulence. EHV-1 strain A3/97 was not neurovirulent despite being detected in the brains of infected BALB/c nude mice. These findings indicate that several inbred mouse strains are susceptible to neuropathogenic EHV-1 strains and should be useful models for studying the pathogenesis and mechanisms contributing to EHV-induced myeloencephalopathy in horses.


Revista Brasileira De Parasitologia Veterinaria | 2012

Detection of Neospora sp. antibodies in cart horses from urban areas of Curitiba, Southern Brazil

Eliana Monteforte Cassaro Villalobos; Keiko Endo Furman; Maria do Carmo Custódio de Souza Hunold Lara; Elenice Maria Sequetin Cunha; Mariane Angélica Pommerening Finger; Ana Paula Brenner Busch; Ivan Roque de Barros Filho; Ivan Deconto; Peterson Triches Dornbusch; Alexander Welker Biondo

Neospora caninum is a protozoan parasite which affects dogs as definitive hosts and several mammalian species as intermediate hosts mainly causing abortions and central nervous system disorders. The reemerging population of cart horses for carrying recycling material in urban areas of major cities in Brazil may have an impact on disease spreading, and these animals may be used as sentinels for environmental surveillance. Thus, the present study investigated the frequency of Neospora sp. antibodies in cart horses from Curitiba and surrounding areas, Paraná State, Southern Brazil. IgG antibodies against Neospora sp. were detected using indirect fluorescence antibody test (IFAT), and titers equal to or higher than 1:50 were considered reactive. Of all samples, 14/97 (14.4%) were positive: 2/29 (6.9%) were younger than 5; 5/26 (19.2%) between 6 and 9; and 6/31 (19.4%) older than 10 years of age. One of the 11 animals with unknown age was positive (9.1%). Cart horses are likely to be more exposed to dog feces and to Neospora sp. oocyst contamination in urban settings and a lower frequency of disease in dogs may have a negative impact on horse infection risk in these areas.


Veterinary Parasitology | 2015

Occurrence of antibodies against Toxoplasma gondii and its isolation and genotyping in donkeys, mules, and horses in Brazil.

Solange Maria Gennari; Patrícia de O. Esmerini; Marcos Lopes; Herbert Sousa Soares; S.N. Vitaliano; Aline Diniz Cabral; Hilda Fátima de Jesus Pena; Mauricio Claudio Horta; Paulo H. Cavalcante; Kleber Portela Fortes; Eliana Monteforte Cassaro Villalobos

The occurrence of antibodies against Toxoplasma gondii was determined in donkeys, mules, and horses from different regions of Brazil. Serum samples from 304 donkeys (67.11%), 118 horses (26.05%), and 31 mules (6.84%) were analyzed by means of the indirect fluorescent antibody test (cutoff=64). Antibodies against T. gondii were detected in 129 equids (28.47%) (82 donkeys, 32 horses, and 15 mules). Tissue samples from 19 seropositive and 50 seronegative animals were obtained in order to isolate the parasite by means of mouse bioassay, and T. gondii was isolated from a donkey. Through genotypic characterization of the isolate, by means of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) using 11 genotypic markers, the genotype #163 (TgCkBr220), which has already been described in chickens in Brazil, was identified.


Arquivo Brasileiro De Medicina Veterinaria E Zootecnia | 2006

Antibodies against pseudorabies virus in feral swine in southeast Brazil

Elenice Maria Siquetin Cunha; Alessandra Figueiredo de Castro Nassar; Maria do Carmo Custódio de Souza Hunold Lara; Josete Garcia Bersano; Eliana Monteforte Cassaro Villalobos; J.C.F. Oliveira

Soros de 358 javalis (Sus scrofa), criados em sistema de semiconfinamento em propriedades do estado de Sao Paulo, foram coletados entre 1998 e 2000 e testados para anticorpos contra o virus da doenca de Aujeszky (VDA), pela tecnica de soroneutralizacao (SN) e ensaio imunoenzimatico (ELISA). Foram detectados animais soropositivos em tres das sete propriedades analisadas. Do total de javalis testados, 30,7% apresentaram anticorpos neutralizantes contra o VDA, com variacao de 25,2% a 100% nas propriedades com animais sororreagentes. O ELISA detectou menor numero de sororeagentes (19,3%), sendo a sensibilidade e a especificidade 57,3% e 97,6%, respectivamente, e a correlacao observada de 85,2% (P<0,0001). Os resultados mostram que a infeccao pelo virus da doenca de Aujeszky ocorre em criacoes de javalis no estado de Sao Paulo, e compromete o sucesso de um futuro programa de erradicacao da doenca na regiao.


Revista Brasileira De Parasitologia Veterinaria | 2013

Detection of anti-Toxoplasma gondii antibodies in carthorses in the metropolitan region of Curitiba, Paraná, Brazil.

Mariane Angélica Pommerening Finger; Eliana Monteforte Cassaro Villalobos; Maria do Carmo Custódio de Souza Hunold Lara; Elenice Maria Sequetin Cunha; Ivan Roque de Barros Filho; Ivan Deconto; Peterson Triches Dornbusch; Leila Sabrina Ullmann; Alexander Welker Biondo

Toxoplasma gondii, the agent for toxoplasmosis, has worldwide distribution. Horses normally play a secondary role in its life cycle, but movement around urban areas, feeding on grass and the increasing use of carthorses for gathering recyclable material in some urban areas of Brazil may increase their exposure to T. gondii infection. The aim of the present study was to investigate the frequency of anti-T. gondii antibodies in carthorses in the metropolitan region of Curitiba, PR. IgG antibodies against T. gondii were detected using the indirect fluorescence antibody test (IFAT) (titers ≥ 64). Seventeen (17.0%) of the 100 horses sampled were seropositive. There were no statistical differences in relation to sex (p = 0.28) or age (p = 0.15). Our findings suggest that carthorses are exposed to T. gondii infections and that no associations with age or sex exist.


BioMed Research International | 2013

Detection of Bovine Leukemia Virus in Brains of Cattle with a Neurological Syndrome: Pathological and Molecular Studies

Rubens Henrique Ramos D'Angelino; Edviges Maristela Pituco; Eliana Monteforte Cassaro Villalobos; Ricardo Harakava; Fabio Gregori; Claudia Del Fava

Bovine leukemia virus (BLV) was investigated in the central nervous system (CNS) of cattle with neurological syndrome. A total of 269 CNS samples were submitted to nested-PCR (BLV env gene gp51), and the viral genotypes were identified. The nested-PCR was positive in 4.8% (13/269) CNS samples, with 2.7% (2/74) presenting at histological examination lesions of nonpurulent meningoencephalitis (NPME), whereas 5.6% (11/195) not presenting NPME (P > 0.05). No samples presented lymphosarcoma. The PCR products (437 bp) were sequenced and submitted to phylogenetic analysis by neighbor-joining and maximum composite likelihood methods, and genotypes 1, 5, and 6 were detected, corroborating other South American studies. The genotype 6 barely described in Brazil and Argentina was more frequently detected in this study. The identity matrices showed maximum similarity (100%) among some samples of this study and one from Argentina (FJ808582), recovered from GenBank. There was no association among the genotypes and NPME lesions.


Arquivos do Instituto Biológico | 2012

Ocorrência de equídeos soropositivos para os vírus das encefalomielites e anemia infecciosa no Estado de Mato Grosso

Rosane Marini Melo; R.C. Cavalcanti; Eliana Monteforte Cassaro Villalobos; Elenice Maria Siquetin Cunha; Maria do Carmo Custódio de Souza Hunold Lara; Daniel Moura de Aguiar

The present work evaluated the occurrence of seropositive equids for eastern encephalomyelitis virus (EEE), western encephalomyelitis virus (WEE), Venezuelan encephalomyelitis virus (VEE) and infectious anemia virus (IAV) in the Pantanal, Savannah and Amazon biomes of Mato Grosso State, Brazil. The detection of antibodies against IAV was carried out in 886 sera of equids by the immunodiffusion test, and antibodies against EEE, WEE and VEE were evaluated in 473 sera by the seroneutralization test performed in VERO cells. The results showed 46 IAV seropositive equids (5.1%). No sera was positive in the Amazon region, and the Pantanal biome showed a higher occurrence with 36.6% of seropositives (P 0.05). Despite that IAV was not observed in equids from the Amazon, the occurrence of seropositive equids agree with the Brazilian prevalence. The differences of EEE and VEE behavior between biomes reinforce the presence of animal reservoirs, along with environmental and weather characteristics that support vector maintenance, which favor the presence of the viral infection in Mato Grosso State.


Revista Brasileira De Parasitologia Veterinaria | 2015

Occurrence of antibodies anti -Toxoplasma gondii, Neospora caninum and Leptospira interrogans in a captive deer herd in Southern Brazil

Cristina Kraemer Zimpel; Ana Laura Grazziotin; Ivan Roque de Barros Filho; Ana M. S. Guimaraes; Leonilda Correia dos Santos; Wanderlei de Moraes; Zalmir Silvino Cubas; Marcos Oliveira; Edviges Maristela Pituco; Maria do Carmo Custódio de Souza Hunold Lara; Eliana Monteforte Cassaro Villalobos; Lília Marcia Paulin Silva; Elenice Maria Sequetin Cunha; Vanessa Castro; Alexander Welker Biondo

A large number of Brazilian zoos keep many endangered species of deer, however, very few disease surveillance studies have been conducted among captive cervids. Blood samples from 32 Brazilian deer (Blastocerus dichotomus, Mazama nana and Mazama americana) kept in captivity at Bela Vista Biological Sanctuary (Foz do Iguaçu, Brazil) were investigated for 10 ruminant pathogens, with the aims of monitoring deer health status and evaluating any potential zoonotic risk. Deer serum samples were tested for Brucella abortus, Leptospira (23 serovars), Toxoplasma gondii, Neospora caninum, bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, foot-and-mouth disease virus, western equine encephalitis virus, eastern equine encephalitis virus and Venezuelan equine encephalitis virus. Antibodies against T. gondii (15.6%), N. caninum (6.2%) and L. interrogans serogroup Serjoe (3.1%) were detected. The serological results for all other infectious agents were negative. The deer were considered to be clinically healthy and asymptomatic regarding any disease. Compared with studies on free-ranging deer, the prevalences of the same agents tested among the captive deer kept at the Sanctuary were lower, thus indicating good sanitary conditions and high-quality management practices at the zoo.


Revista Do Instituto De Medicina Tropical De Sao Paulo | 2014

PREVALENCE OF ANTIBODIES AGAINST INFLUENZA VIRUS IN NON-VACCINATED EQUINES FROM THE BRAZILIAN PANTANAL

Lucas Gaíva E Silva; Alice M.C.M. Borges; Eliana Monteforte Cassaro Villalobos; Maria do Carmo Custódio de Souza Hunold Lara; Elenice Maria Siquetin Cunha; Anderson Castro Soares de Oliveira; Ísis Assis Braga; Daniel Moura de Aguiar

The prevalence of antibodies against Equine Influenza Virus (EIV) was determined in 529 equines living on ranches in the municipality of Poconé, Pantanal area of Brazil, by means of the hemagglutination inhibition test, using subtype H3N8 as antigen. The distribution and possible association among positive animal and ranches were evaluated by the chi-square test, spatial autoregressive and multiple linear regression models. The prevalence of antibodies against EIV was estimated at 45.2% (95% CI 30.2 - 61.1%) with titers ranging from 20 to 1,280 HAU. Seropositive equines were found on 92.0% of the surveyed ranches. Equine from non-flooded ranches (66.5%) and negativity in equine infectious anemia virus (EIAV) (61.7%) were associated with antibodies against EIV. No spatial correlation was found among the ranches, but the ones located in non-flooded areas were associated with antibodies against EIV. A negative correlation was found between the prevalence of antibodies against EIV and the presence of EIAV positive animals on the ranches. The high prevalence of antibodies against EIV detected in this study suggests that the virus is circulating among the animals, and this statistical analysis indicates that the movement and aggregation of animals are factors associated to the transmission of the virus in the region.


Revista Brasileira De Parasitologia Veterinaria | 2014

Occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in Barbary sheep at Curitiba zoo, southern Brazil.

Vivien Midori Morikawa; Cristina Kraemer Zimpel; Igor Adolfo Dexheimer Paploski; Maria do Carmo Custódio de Souza Hunold Lara; Eliana Monteforte Cassaro Villalobos; Adriana Hellmeister de Campos Nogueira Romaldini; Liria Hiromi Okuda; Alexander Welker Biondo; Ivan Roque de Barros Filho

Barbary sheep (Ammotragus lervia) have the potential to act as hosts of important infectious diseases, particularly zoonoses. Blood samples from 17 Barbary sheep at the Curitiba zoo were collected to evaluate occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies, tested using the indirect immunofluorescence antibody test (IFAT). Anti-T. gondii and anti-N. caninum antibodies were detected in 4/17 (23.5%) and 4/17 (23.5%) samples, respectively. The present study has shown that Barbary sheep at Curitiba zoo were exposed to T. gondii and N. caninum and therefore may act as intermediate hosts, spreading toxoplasmosis and neosporosis within and between species in shared areas.

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Enio Mori

University of São Paulo

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