Elisa Giambanelli

Wastes generated during the storage of extra virgin olive oil as a natural source of phenolic compounds.

Phenolic compounds in extra virgin olive oil (EVOO) have been associated with beneficial effects for health. Indeed, these compounds exert strong antiproliferative effects on many pathological processes, which has stimulated chemical characterization of the large quantities of wastes generated during olive oil production. In this investigation, the potential of byproducts generated during storage of EVOO as a natural source of antioxidant compounds has been evaluated using solid-liquid and liquid-liquid extraction processes followed by rapid resolution liquid chromatography (RRLC) coupled to electrospray time-of-flight and ion trap mass spectrometry (TOF/IT-MS). These wastes contain polyphenols belonging to different classes such as phenolic acids and alcohols, secoiridoids, lignans, and flavones. The relationship between phenolic and derived compounds has been tentatively established on the basis of proposed degradation pathways. Finally, qualitative and quantitative characterizations of solid and aqueous wastes suggest that these byproducts can be considered an important natural source of phenolic compounds, mainly hydroxytyrosol, tyrosol, decarboxymethyl oleuropein aglycone, and luteolin, which, after suitable purification, could be used as food antioxidants or as ingredients in nutraceutical products due to their interesting technological and pharmaceutical properties.

A comparative study of bioactive compounds in primitive wheat populations from Italy, Turkey, Georgia, Bulgaria and Armenia

BACKGROUND In recent years there has been a considerable interest in the consumption of ancient wheats, often referred to as having superior health-promoting properties than modern cultivars. The BaSeFood project allowed us to explore the use of primitive wheats in the Black Sea area region and in Italy, with special regard to emmer (Triticum dicoccum) and einkorn (T. monococcum), and to collect seed samples to be grown and compared for their bioactive content, together with some other primitive wheat genotypes (T. timopheevi, T. palaeo-colchicum, T. macha). RESULTS The data show that genotype was an important factor controlling phytochemical content. Variability ranges were as follows: lipids (18.0-28.5 g kg(-1)), tocols (26.6-72.8 mg kg(-1)), carotenoids (1.6-8.4 mg kg(-1)), sterols (441-929 mg kg(-1)) and phenolic compounds (819-1465 mg kg(-1)) content (dry matter basis). The fraction of individual components, within each class, was also variable; however, the species were well discriminated by their overall composition. CONCLUSIONS The present research represents a further contribution to the available literature about the analytical composition of primitive wheats, including the complete range of relevant bioactives and lesser investigated species. The data do not support an overall superiority of primitive forms, but evidenced interesting, potentially exploitable, between- and within-species variability.

Comparison of leafy kale populations from Italy, Portugal, and Turkey for their bioactive compound content: phenolics, glucosinolates, carotenoids, and chlorophylls

BACKGROUND Kales are primitive leafy Brassica oleracea L. forms, widespread in local farming systems of several European countries and employed in the preparation of traditional recipes. Kales are also potential sources of healthy bioactive phytochemical components. The present study compared the bioactive compound content of kale populations from Italy, Portugal, and Turkey, either from local sources or grown in an experimental field. RESULTS Total phenolics, glucosinolates (GLS), carotenoids, and chlorophylls were in the ranges 8310-38 110, 755-8580, 135-2354, and 1740-16,924 mg kg(-1) dry matter, respectively. On average, locally harvested samples showed a total GLS content about twice as high as populations from the experiment. Conversely, pigments were significantly more abundant in experimental than in local kales, owing to the higher soil fertility. Portuguese samples showed higher phenolic and GLS amounts than Italian and Turkish kales, whereas some of the Italian samples were the richest in carotenoids. CONCLUSION This paper represented the first cross-country comparison of local kale accessions with respect to bioactive compound amounts. Both geographic origin and growing environment appeared to be remarkable and discriminating factors in determining bioactive levels in leafy kales, with possible effects on their health-promoting and sensorial attributes.

Broccoli glucosinolate degradation is reduced performing thermal treatment in binary systems with other food ingredients

Glucosinolate (GL) stability has been widely studied in different Brassica species. However, the matrix effect determined by the presence of other ingredients occurred in many broccoli-based traditional recipes may affect GL thermal degradation. In this study, the matrix effect on GL thermal degradation was investigated by means of binary systems containing broccoli and another ingredient such as potato, corn starch, lentils protein or onion. Data showed that in binary systems the GL degradation was lower compared to the only-broccoli system, in particular in the broccoli/onion systems. The kinetics of GL degradation in broccoli/onion systems at different ratios showed that the higher the amount of onion, the higher the protective effect and that GL thermal degradation followed a second order model. Finally the possibility that the effect was related to the amount of flavonoids present in onions was ruled out by data obtained using broccoli/onion systems made with three onion varieties having different flavonoid content. This study shows for the first time that the presence of other food ingredients can efficiently reduce GL thermal degradation. The protective effect of onion, often present in the traditional recipes of broccoli soups in many countries, points out that the interaction of different ingredients may not only improve the taste of a dish, but also the healthiness.

An on-site comparative study of yield factors during glume removal, a primary step in the traditional processing of hulled wheats

BACKGROUND Glume removal represents the preliminary step of hulled wheat grain processing for human consumption. Einkorn and emmer wheat are two hulled species, cultivated in marginal areas, from the Mediterranean to the Caucasian region, and the subject of investigation in this paper. An experiment has been carried out to compare the yield of the traditional process, used in Turkey and Armenia, and an updated Italian procedure, by means of on-plant samplings. RESULTS Crushed grains represent a product from all the plants examined. However, the relative amounts of fractions of different size and use, according to each country, varied consistently. The main Italian product is whole pearled grain. The calculated yield factors varied in the following ranges: dehulling: 0.73-0.81 kg kg(-1); food product: 0.82-0.96 kg kg(-1); overall yield: 0.56-0.95 kg kg(-1) excluding unwanted losses, and 0.42-0.74 kg kg(-1) including unwanted losses. CONCLUSIONS This first comparative assay of hulled wheat processing showed that, not considering the kind of product obtained, glume removal can be carried out with good efficiency in continuously operating traditional plants. Modern schemes, however, allow yielding of intact kernels. Some yield factors were highly affected by the available technology, especially when the process was operating discontinuously.

Identification and quantification of phenolic compounds in edible wild leafy vegetables by UHPLC/Orbitrap‐MS

BACKGROUND A recent interest in edible wild leafy vegetables has been documented. Consumers often associate these species with health promotion. In this study, several wild species of the Asteraceae family and Knautia integrifolia (Dipsacaceae) were locally documented for their use in traditional cuisine and sampled from the wild. RESULTS Phenolic compounds were identified and quantified by ultra-high-performance liquid chromatography coupled to Orbitrap high-resolution mass spectrometry. Hydroxycinnamic acids ranging from 1388 to 53 076 mg kg-1 dry weight (DW) were the most abundant compounds in all species (69-98% of the total phenolic content) except Tragopogon pratensis. Thirty compounds were identified as flavonoids, mostly as glycosidic forms of luteolin, apigenin, kaempferol and quercetin. The sum of flavonoids ranged between 212 and 12 598 mg kg-1 DW; they represented 65% of the total phenolic content for T. pratensis. Three anthocyanins were detected, representing in most cases less than 1% of the total phenolic content (3-627 mg kg-1 DW). Higher anthocyanin contents were observed for Cichorium types. CONCLUSION Different phenolic profiles were observed between species, especially considering the class of flavonoids. Individual species may be of some interest for their content of specific minor flavonoids.

Fennel (Foeniculum vulgare Mill. subsp. piperitum) florets, a traditional culinary spice in Italy: evaluation of phenolics and volatiles in local populations, and comparison with the composition of other plant parts

BACKGROUND Wild fennel (Foeniculum vulgare Mill. subsp. piperitum) florets are used as a typical spice in central and southern Italy. Although fennel (Foeniculum vulgare Mill.), belonging to the Apiaceae (syn. Umbelliferae) family, is a well-known vegetable and aromatic plant, whose main phytochemical compounds have been extensively analysed and investigated as flavouring agents and for their putative health promoting functions, its florets have not been specifically considered up to now. Therefore, the volatile and phenolic composition of florets from an Italian wild fennel crop was determined at different developmental stages, and compared to that of leaves and fruits. Moreover, florets of nine Italian wild fennel populations of different geographical origin from northern-central Italy were also analysed. RESULTS The total phenolic amount increased from leaves to florets, reaching its highest value in early florets, at 58 012 mg kg-1 of dry matter (DM), then constantly decreased in fruits. In florets of wild populations, phenolics ranged from 6666 to 43 368 mg kg-1 DM. The total amount of volatile compounds was more than twice higher in florets (21 449 mg kg-1 DM) than in leaves (10 470 mg kg-1 DM), reaching its highest value in fruits (50 533 mg kg-1 DM). Estragole and trans-anethole were the main compounds of the volatile fraction. Total volatiles ranged from 24 367 to 60 468 mg kg-1 DM in florets of local populations. CONCLUSION Significant changes in the total amount and profile of both phenolic and volatile compounds occurred during plant development. The consistent increase of estragole at later developmental stages supported the claim of different sensory properties of florets and fruits. Geographical origin significantly affected phenolic and volatile composition of wild fennel florets.