Elisa Pellegrino

At the Root of the Wood Wide Web: Self Recognition and Nonself Incompatibility in Mycorrhizal Networks

Arbuscular mycorrhizal (AM) fungi are mutualistic symbionts living in the roots of 80% of land plant species, and developing extensive, belowground extraradical hyphae fundamental for the uptake of soil nutrients and their transfer to host plants. Since AM fungi have a wide host range, they are able to colonize and interconnect contiguous plants by means of hyphae extending from one root system to another. Such hyphae may fuse due to the widespread occurrence of anastomoses, whose formation depends on a highly regulated mechanism of self recognition. Here, we examine evidences of self recognition and nonself incompatibility in hyphal networks formed by AM fungi and discuss recent results showing that the root systems of plants belonging to different species, genera and families may be connected by means of anastomosis formation between extraradical mycorrhizal networks, which can create indefinitely large numbers of belowground fungal linkages within plant communities.

Establishment, persistence and effectiveness of arbuscular mycorrhizal fungal inoculants in the field revealed using molecular genetic tracing and measurement of yield components

• Inoculation of crop plants by non-native strains of arbuscular mycorrhizal (AM) fungi as bio-enhancers is promoted without clear evidence for symbiotic effectiveness and fungal persistence. To address such gaps, the forage legume Medicago sativa was inoculated in an agronomic field trial with two isolates of Funneliformis mosseae differing in their nuclear rDNA sequences from native strains. • The inoculants were traced by PCR with a novel combination of the universal fungal NS31 and Glomeromycota-specific LSUGlom1 primers which target the nuclear rDNA cistron. The amplicons were classified by restriction fragment length polymorphism and sequencing. • The two applied fungal inoculants were successfully traced and discriminated from native strains in roots sampled from the field up to 2 yr post inoculation. Moreover, field inoculation with inocula of non-native isolates of F. mosseae appeared to have stimulated root colonization and yield of M. sativa. • Proof of inoculation success and sustained positive effects on biomass production and quality of M. sativa crop plants hold promise for the role that AM fungal inoculants could play in agriculture.

Temporal trends in extreme rainfall intensity and erosivity in the Mediterranean region: a case study in southern Tuscany, Italy

Worldwide climate is likely to become more variable or extreme with increases in intense precipitation. In Mediterranean areas, climate change will increase the risks of droughts, flash floods and soil erosion. Despite rainfall intensity being a key factor in erosive processes, in these areas information on extreme rainfall intensity and the associated erosivity, based on high-temporal resolution data, is either non homogeneous or scarce. These parameters thus need to be assessed in order to highlight suitable adaptation strategies. In this paper, an hourly rainfall intensity (RI) data series is analyzed together with the corresponding 1-min rainfall intensity maximum (RIm) of 23 rainfall gauges located in Tuscany, Italy, in an area highly vulnerable to erosion. The aim is to look for temporal trends (1989–2010) in extreme rainfall intensity and erosivity. Fixed effect logistic regression shows statistically significant temporal increases in the number of RI and RIm exceedances over the 95th percentile threshold. Winter is shown to be the season with the strongest increasing trend in coastal and inland rainfall gauge groups, followed by spring for the coastal group and autumn for the inland group. Linear regressions show that in the inland group there is a temporal increase in rainfall erosivity and on a seasonal basis, the highest increase is observed in autumn. By contrast, for the coastal group this increasing trend is only detectable for spring and winter. Such an increase in rainfall erosivity and its potential continuation could have a strong adverse effect on Mediterranean land conservation.

Biofortification with Iron and Zinc Improves Nutritional and Nutraceutical Properties of Common Wheat Flour and Bread

The effect of field foliar Fe and Zn biofortification on concentration and potential bioavailability of Fe and Zn and health-promoting compounds was studied in wholemeal flour of two common wheat varieties (old vs modern). Moreover, the effect of milling and bread making was studied. Biofortification increased the concentration of Zn (+78%) and its bioavailability (+48%) in the flour of the old variety, whereas it was ineffective in increasing Fe concentration in both varieties. However, the old variety showed higher concentration (+41%) and bioavailability (+26%) of Fe than the modern one. As regard milling, wholemeal flour had higher Fe, Zn concentration and health-promoting compounds compared to white flour. Bread making slightly change Fe and Zn concentration but greatly increased their bioavailability (77 and 70%, respectively). All these results are of great support for developing a production chain of enriched functional bread having a protective role against chronic cardio-vascular diseases.

Author Correction: Impact of genetically engineered maize on agronomic, environmental and toxicological traits: a meta-analysis of 21 years of field data

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.