Elisabetta Carico
Sapienza University of Rome
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Elisabetta Carico.
International Journal of Gynecological Pathology | 2011
Giovanni Negri; Giulia Bellisano; Elisabetta Carico; Gavino Faa; Armin Kasal; Sonia Antoniazzi; Eduard Egarter-Vigl; Andrea Piccin; Paolo Palma; Fabio Vittadello
Although the diagnostic criteria of in-situ and invasive adenocarcinomas of the cervix uteri are well established, the differentiation from benign mimics may be difficult and the morphologic features of the precursors of endocervical adenocarcinoma are still debated. In this study, we evaluated the usefulness of p16ink4a (p16), ProEX C, and Ki-67 for the diagnosis of endocervical adenocarcinoma and its precursors. Immunohistochemistry with p16, ProEX C, and Ki-67 was performed in 82 glandular lesions including 15 invasive adenocarcinomas, 29 adenocarcinomas in situ (AIS), 22 non-neoplastic samples, and 16 cases of glandular dysplasia (GD), which showed significant nuclear abnormalities but did not meet the diagnostic criteria for AIS. The immunohistochemical expression pattern was scored according to the percentage of the stained cells (0, 1+, 2+, and 3+ when 0% to 5%, 6% to 25%, 26% to 50%, and more than 50% of the cells were stained, respectively) and was evaluated for each antibody. p16 was at least focally expressed (1+ or more) in 14 of 15 invasive adenocarcinomas, in all AIS and in 7 negative samples. ProEX C and Ki-67 both scored 1+ or more in all adenocarcinomas and AIS and in 8 and 6 negative samples, respectively. Of the GD 15, 14, and 15 expressed p16, ProEX C, and Ki-67, respectively. The score differences between neoplastic and non-neoplastic samples were highly significant for each marker (P<0.001); however, the score distribution by marker differed significantly only in GD (P=0.006) in which, compared with the other markers, p16 showed more often a 3+ pattern. Our study shows that p16, Ki-67, and ProEX C may be helpful for the diagnosis of glandular lesions of the cervix uteri and may also improve the diagnostic accuracy of endocervical GD. In particularly problematic cases, the combination of p16 and a proliferation marker can provide additional help for the interpretation of these lesions.
Oncotarget | 2015
Marta Nardella; Loredana Guglielmi; Carla Musa; Ilaria Iannetti; Giovanna Maresca; Donatella Amendola; Manuela Porru; Elisabetta Carico; Giuseppe Sessa; Rosalba Camerlingo; Carlo Dominici; Francesca Megiorni; Marika Milan; Claudia Bearzi; Roberto Rizzi; Giuseppe Pirozzi; Carlo Leonetti; Barbara Bucci; Delio Mercanti; Armando Felsani; Igea D'Agnano
Tumor-initiating cells constitute a population within a tumor mass that shares properties with normal stem cells and is considered responsible for therapy failure in many cancers. We have previously demonstrated that knockdown of the nuclear envelope component Lamin A/C in human neuroblastoma cells inhibits retinoic acid-mediated differentiation and results in a more aggressive phenotype. In addition, Lamin A/C is often lost in advanced tumors and changes in the nuclear envelope composition occur during tumor progression. Based on our previous data and considering that Lamin A/C is expressed in differentiated tissues, we hypothesize that the lack of Lamin A/C could predispose cells toward a stem-like phenotype, thus influencing the development of tumor-initiating cells in neuroblastoma. This paper demonstrates that knockdown of Lamin A/C triggers the development of a tumor-initiating cell population with self-renewing features in human neuroblastoma cells. We also demonstrates that the development of TICs is due to an increased expression of MYCN gene and that in neuroblastoma exists an inverse relationship between LMNA and MYCN expression.
Virchows Archiv | 2009
Elisabetta Carico; Franco Fulciniti; Maria Rosaria Giovagnoli; Nunzia Simona Losito; Gerardo Botti; Giulio Benincasa; Maria Giuseppina Farnetano; Aldo Vecchione
An immunohistochemical (IHC) study has been conducted on 34 cases of untreated endocervical adenocarcinomas collected among three institutions (Ospedale S. Andrea, Rome; Istituto Nazionale Tumori “Fondazione G. Pascale”, Naples; and Clinica Malzoni, Avellino). The E-cadherin and α- and β-catenin complex status has been investigated along with p16INK4a in all studied cases with the aim to study whether the pattern of expression of the cadherin–catenin complex could be causally related to the expression of P16INK4a protein. Results were evaluated for statistical significance by a non-parametric test (Kruskal–Wallis). Endocervical adenocarcinomas as a group were uniformly expressing p16INK4a except for two cases, and all lesions displayed downregulation of the cadherin–catenin complex, without demonstrating statistically significant differences among the different histotypes. The lack of nuclear accumulation of β-catenin found in this group of lesions probably implies that no alteration of the β-catenin/Wnt metabolic pathway is present in endocervical adenocarcinoma, as opposed to what is found in the literature for squamous carcinoma of the cervix. The diffuse expression of p16INK4a protein in this group of neoplasms stresses the important role of high-risk human papillomavirus infection in neoplastic causation possibly via the viral E7-mediated inactivation of pRB tumor-suppressor protein and also underlines the useful role of p16INK4a immunostaining in the diagnostic algorithm of endocervical adenocarcinomas. In consideration of these findings, investigation of downstream β-catenin genes c-myc and cyclin D1 is sought as possibly contributive in the molecular pathogenesis of endocervical adenocarcinoma.
Cancer Cytopathology | 2017
Maria Benevolo; Elena Allia; Daniela Gustinucci; Francesca Rollo; Simonetta Bulletti; Elena Cesarini; Basilio Passamonti; Maria Rosaria Giovagnoli; Elisabetta Carico; Francesca Carozzi; Alessandra Mongia; Giulia Fantacci; Massimo Confortini; Teresa Rubino; Cristina Fodero; Sonia Prandi; Natalina Marchi; Angelo Farruggio; Anna Coccia; Luigia Macrì; Bruno Ghiringhello; Guglielmo Ronco; Emma Bragantini; Enzo Polla; Vincenzo Maccallini; Giovanni Negri; Paolo Giorgi Rossi
The accumulation of cyclin‐dependent kinase inhibitor 2A (p16ink4a) protein in a cell is associated with neoplastic progression in precancerous cervical lesions. Dual staining for p16ink4a and Ki‐67 has been proposed as a triage test in cervical cancer screening for women who test positive for human papillomavirus DNA. In this study, interobserver reproducibility of the interpretation of this test was assessed.
Stem Cell Research & Therapy | 2014
Donatella Amendola; Marta Nardella; Loredana Guglielmi; Lidia Cerquetti; Elisabetta Carico; Viola Alesi; Manuela Porru; Carlo Leonetti; Claudia Bearzi; Roberto Rizzi; Igea D’Agnano; Antonio Stigliano; Giuseppe Novelli; Barbara Bucci
IntroductionThe cancer stem cell model links neoplastic cells with normal stem cell biology, but little is known on how normal stem cells are transformed into cancer stem cells.MethodsTo investigate the processes underlying the transformation of normal stem cells we developed in vitro a cancer stem cell model from human amniotic and chorionic placenta membranes. In this model we studied the expression of specific stem cell molecules by flow cytometry, and genes, by real time RT-PCR. Microscopy immunfluorescence was employed to investigate the proliferative and differentiation patterns. Fluorescence microscopy and FACS were employed to investigate the proliferative and differentiation patterns. To evaluate the tumorigenic potential of our model we injected the cells into NOD.CB17-Prkdcscid/NCrHsd mice.ResultsNormal human stem cells from amniotic and chorionic placenta membranes were converted into neural cell lineages, under specific conditions, to form secondary neurospheres with a capacity for self-renewal. After extensive in vitro culture, these cells underwent spontaneous transformations and acquired a neuroblastoma (NB)-like phenotype with an elevated proliferative potential that is comparable to established neuroblastoma cell lines. The ability of these cells to transform their phenotype was evidenced by increased clonogenic ability in vitro; by augmented expression level of certain proliferation- and transformation-related genes (e.g., CCNA2, MYCN, ENPP2, GRIA3, and KIT); by the presence of multinucleated and hyperdiploid cells. We further demonstrated that the transformed phenotype is an NB by measuring the expression of NB-specific markers, disialoganglioside GD2 and N-Myc proteins.ConclusionsWe have developed a cancer stem cell model starting from normal human stem cells derived from amniotic and chorionic placenta membranes. These cells are able to differentiate into neural cell lineages and to undergo spontaneous transformations and acquire an NB-like phenotype.
Journal of Cytology and Histology | 2017
Ilaria Lamberti; Adriana Bonifacino; Stefania Scarpino; Sandra Villani; Rita Mancini; Elisabetta Carico; Maria Rosaria Giovagnoli; Enrico Giarnieri
Mucinous carcinoma of the male breast is an uncommon malignant breast neoplasm and its diagnoses remain difficult. It is probably due to such a low rate of breast cancer cases that men tend to be diagnosed at an older age than women and with a later stage of the disease. We describe a case of a 69-year-old male who displayed a palpable lump in his right axilla several years ago, showing signs of cutaneous adnexal mucinous adenocarcinoma after biopsy. After six years and several clinical examination and systemic investigation without results, the patient underwent to fine needle aspiration cytology and subsequently a biopsy of a mass with irregular margins in the retroareolar region of his right breast. The final diagnosis was of a mixed mucinous breast cancer with neuroendocrine differentiation. The tumor cells phenotype showed Synaptophisin (+), CEA (+/-), CK-20 (-), CK-7 (+), TTF-1 (-), estrogen receptor (-), progesterone (-) and HER 2 (++). These results were unusual for a mucinous male breast carcinoma. In the presence of a lesion in the axillary area with no specific primary origin, breast cancer should never be ruled out, even in the absence of clinical evidence and with an immunohistochemical pattern not indicative of mammary origin.
Edorium Journal of Oncocytology | 2015
Enrico Giarnieri; Antonella Carnuccio; Domenico Galasso; Rita Mancini; Elisabetta Carico; Emilio Di Giulio; Maria Rosaria Giovagnoli
Most of pancreatic masses are represented by neoplastic processes, primarily ductal adenocarcinoma and less frequently neuroendocrine tumor, lymphoma and metastasis. On the contrary, non-neoplastic lesions are represented by chronic, autoimmune pancreatitis and cysts [1, 2]. Pancreatic cancer is the fourth and fifth most common cancer in men and women, respectively [3]. Due to local invasion or distant metastasis, only 15–20% of patients are surgical candidates at presentation. Among them, the five-year survival rate is only 10–15% [4]. About 70% of pancreatic cancers develop in the head while 10– 20% in the body and tail [5]. In many cases, pancreatic cancer is diagnosed in the advanced stage of the disease and at this point the tumor cannot be surgically excised. In fact, at the moment of diagnosis, 52% patients show distant disease and 26% have regional spread [6]. Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) is a rapid, safe, cost-effective and accurate technique to evaluate and stage pancreatic tumors [7]. In addition, this technique has proved a very useful in discriminating between suspicious lesions, inflammation and cancer, especially adenocarcinomas. The EUS-FNA demonstrates a low percentage of major complications and a low risk of disseminating neoplastic cells when compared to the percutaneous approach. Cytological evaluation may have some disadvantages such as a limited
Anticancer Research | 1997
Barbara Bucci; Igea D'Agnano; Claudio Botti; Marcella Mottolese; Elisabetta Carico; Gabriella Zupi; Aldo Vecchione
Gynecologic Oncology | 2001
Elisabetta Carico; M. Atlante; Barbara Bucci; I. Nofroni; Aldo Vecchione
Gynecologic Oncology | 1993
Elisabetta Carico; Deborah French; Barbara Bucci; Rita Falcioni; Aldo Vecchione; Renato Mariani-Costantini