Elizabeth J. Harbron
College of William & Mary
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Publication
Featured researches published by Elizabeth J. Harbron.
Analytical Chemistry | 2012
Elizabeth S. Childress; Courtney A. Roberts; Desmarie Y. Sherwood; Clare L.M. LeGuyader; Elizabeth J. Harbron
We present dye-doped polymer nanoparticles that are able to detect mercury in aqueous solution at parts per billion levels via fluorescence resonance energy transfer (FRET). The nanoparticles are prepared by reprecipitation of highly fluorescent conjugated polymers in water and are stable in aqueous suspension. They are doped with rhodamine spirolactam dyes that are nonfluorescent until they encounter mercury ions, which promote an irreversible reaction that converts the dyes to fluorescent rhodamines. The rhodamine dyes act as FRET acceptors for the fluorescent nanoparticles, and the ratio of nanoparticle-to-rhodamine fluorescence intensities functions as a ratiometric fluorescence chemodosimeter for mercury. The light harvesting capability of the conjugated polymer nanoparticles enhances the fluorescence intensity of the rhodamine dyes by a factor of 10, enabling sensitive detection of mercury ions in water at levels as low as 0.7 parts per billion.
Langmuir | 2014
Christian F. Chamberlayne; Elena A. Lepekhina; Brooklynd D. Saar; Kathryn A. Peth; Jordan T. Walk; Elizabeth J. Harbron
The emission of conjugated polymer nanoparticles (CPNs or Pdots) is often tailored for specific uses by functionalizing CPNs with dyes that act as fluorescence resonance energy transfer (FRET) acceptors. A number of dye functionalization methods for CPNs have been developed, ranging from simple noncovalent doping to covalent attachment. We seek to develop guidelines for when noncovalent doping is acceptable and when covalent attachment is necessary to achieve the desired result. We present results of CPNs functionalized with photochromic spirooxazines by four different methods: simple doping, doping with an amphiphilic coating polymer, covalent functionalization prior to CPN formation, and covalent functionalization after CPN formation. The different CPNs are evaluated in terms of their fluorescence photomodulation properties to determine how the preparation method affects the CPN-dye photophysical interactions. Doping preparations yield the most efficient quenching of CPN emission due to shorter donor-acceptor distances in these CPNs compared to those with covalently tethered dyes. Aging studies reveal that the photochromic dyes in doped samples degrade over time to a far greater extent than those in covalently functionalized samples. These results suggest that dye-doped CPNs are appropriate for short-term experiments where highly efficient FRET is desired while covalent dye functionalization is a better choice for experiments executed over an extended time frame.
Chemistry: A European Journal | 2017
Sarah G. Stratton; Grace H. Taumoefolau; Grace E. Purnell; Mona Rasooly; William L. Czaplyski; Elizabeth J. Harbron
Rhodamine spirolactams (RSLs) have recently emerged as popular fluorescent pH probes due to their fluorescence turn-on capability and ease of functionalization at the spirolactam nitrogen. Design of RSLs is often driven by biological targeting or compatibility concerns, rather than the pH sensitivity of the probe, and the relationship between RSL structure and pKa is not well understood. To elucidate the relationship between pKa values and the properties of substituents attached to the spirolactam nitrogen, a series of 19 aniline-derived RSLs is presented. RSLs derived from di-ortho-substituted anilines exhibit pKa tunability across the moderately acidic region (ca. pH 4-6). Evaluation of pKa data using the Fujita-Nishioka model for ortho substituent effects reveals that both steric and electronic substituent properties influence RSL pH responsiveness, with pKa values increasing as substituent size and electron withdrawing character increase. These trends are attributed to changes in the RSL structure induced by large substituents, and to electronic influences on the protonated spirocyclic reaction intermediate. To demonstrate the practical applicability of these probes in completely aqueous environments, RSL-doped conjugated polymer nanoparticles that exhibit a ratiometric fluorescence response to changing pH levels are presented.
Molecules | 2017
Jordan K. Villa; Hong-Anh Tran; Megha Vipani; Stephanie Gianturco; Konark Bhasin; Brent L. Russell; Elizabeth J. Harbron; Douglas D. Young
The ability to modulate protein function through minimal perturbations to amino acid structure represents an ideal mechanism to engineer optimized proteins. Due to the novel spectroscopic properties of green fluorescent protein, it has found widespread application as a reporter protein throughout the fields of biology and chemistry. Using site-specific amino acid mutagenesis, we have incorporated various fluorotyrosine residues directly into the fluorophore of the protein, altering the fluorescence and shifting the pKa of the phenolic proton associated with the fluorophore. Relative to wild type GFP, the fluorescence spectrum of the protein is altered with each additional fluorine atom, and the mutant GFPs have the potential to be employed as pH sensors due to the altered electronic properties of the fluorine atoms.
Journal of Physical Chemistry C | 2009
Elizabeth J. Harbron; Christina M. Davis; Joshua K. Campbell; Rebecca M. Allred; Marissa T. Kovary; Nicholas J. Economou
Journal of Physical Chemistry C | 2011
Christina M. Davis; Elizabeth S. Childress; Elizabeth J. Harbron
Journal of Physical Chemistry A | 2005
Elizabeth J. Harbron; Diego A. Vicente; Deana H. Hadley; Matthew R. Imm
Journal of Physical Chemistry A | 2000
Douglas S. English; Elizabeth J. Harbron; Paul F. Barbara
Journal of Physical Chemistry B | 2004
Elizabeth J. Harbron; Diego A. Vicente; Mirth T. Hoyt
Journal of Physical Chemistry C | 2007
Steven M. Lewis and; Elizabeth J. Harbron