Ellen Carine Neves Valente

Evaluation of entomopathogenic fungi as biological control agents Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Acari: Ixodidae)

El objetivo de este trabajo fue evaluar diferentes colonias aisladas en concentraciones de Metarhizium anisopliae (Metschnikoff, 1879) Sorokin, 1883 y de Beauveria bassiana (Balsamo) Vuillemim, 1912 para el control de la garrapata comun del vacuno. La multiplicacion de conidias fue realizada a partir de hongos inoculados en arroz precocido esteril. Los ensayos de inmersion fueron realizados sobre garrapatas adultas ingurgitadas o teleoginas colectadas de bovinos no tratados con acaricidas. El diseno experimental fue completamente al azar con 20 tratamientos y cinco repeticiones, siendo cada una de estas constituidas por cinco hembras. La mortalidad de las teleoginas ocasionada por M. anisopliae fue variable entre 92 a 100% y para B. bassiana este parametro vario entre 44 a 100%, observandose que la mortalidad fue superior en las concentraciones de 108 y 109 conidias mL-1, destacandose las cepas de B. bassiana Fitossan 1 e M. anisopliae PL 43 (ambos 109 conidias mL-1) y Fitossan 4 (108 conidias mL-1), que a los 14 dias de tratamiento habian eliminado el 100, 92 y 88 % de las teleoginas, respectivamente. En general, no existio diferencia en relacion al peso de los huevos provenientes de hembras tratadas con el mismo aislado en las concentraciones de 107, 108 y 109 conidia .mL-1, aunque se obtuvieron pesos inferiores para las cepas de M. anisopliae. La eficiencia del control vario de 0% (control) a 31,30% (M. anisopliae Fitossan 4, 108 conidias.mL-1).

Desempenho de Trichogramma galloi (Hymenoptera: Trichogrammatidae) sobre ovos de Diatraea spp. (Lepidoptera: Crambidae)

The objective of this work was to evaluate the performance of Trichogramma galloi (Hymenoptera: Trichogrammatidae) on eggs of Diatraea flavipennella and D. saccharalis (Lepidoptera: Crambidae), as well as the biological characteristics of the parasitoid in both hosts, and to elaborate the fertility life table. For this, tests with and without choice and bioassays to evaluate the biological characteristics of the parasitoid in both hosts were performed. The fertility life table was developed through daily parasitism of T. galloi females on the hosts. The percentage results of parasitism in free-choice tests showed a better acceptance by T. galloi concerning eggs of D. flavipennella. Regarding the biological parameters of the parasitoid in both species, there was no significant difference. Fertility life table results show that D. flavipennella and D. saccharalis allow population increase for T. galloi. The parasitoid T. galloi has satisfactory performance in both hosts, and is a potential control agent for these species.

Primeiro registro de Pachycoris torridus (Scopoli, 1772) (Hemiptera: Scutelleridae) em pinhão-manso (Euphorbiaceae) em Alagoas, Brasil

The occurrence of Pachycoris torridus (Scopoli) (Hemiptera: Scutelleridae) in Igaci (in the state of Alagoas, Brazil), is observed attacking the physic nut (Jatropha curcas L.) The insect, until then not verified in the region, takes economic relevancy considering the nature of damage, causing infestation in 100% plants.

REGISTRO DE Xanthopastis timais EM AMARÍLIS E EFEITO DO EXTRATO DE GRAVIOLA NA MORTALIDADE LARVAL

The aim of this study was to report the occurrence of Xanthopastis timais (Cramer, 1780) (Lepidoptera: Noctuidae) in amaryllis (Hippeastrum hybridum Hort., Amaryllidaceae), as well as estimating lethal concentrations (LC50 and LC99) of soursop seed extract, Annona muricata L. (Annonaceae), against its larvae. The experiment was carried out at the Laboratory of Entomology of the Research Center for Agricultural Sciences, Federal University of Alagoas, in Rio Largo-AL, Brazil. First, we collected caterpillars from amaryllis plants that were reared until pupal and adult stages (parental generation). Next, part of the adults was used for species identification, and the rest were bred to produce next generation (F1), being after that applied in a laboratory bioassay. The lab trial was performed in a completely randomized design, with six treatments and five replications containing three second-instar caterpillars. These larvae were fed pieces of amaryllis leaf (4 x 4 cm), which were previously soaked in treatments for 30 seconds and, air-dried on absorbent papers for one hour. Each treatment consisted of one concentration of ethanol extract of soursop seeds (0.0, 1.0, 0.5, 0.25, 0.125 and 0.0625% w/ v) with distilled water and DMSO at 1% (v/ v), for solutions. We noted that the lethal concentrations LC50 and LC99 were 0.29% (w/ v) and 2.27% (w/ v), respectively. In view of our results, we can state that larval stage of X. timais were influenced by extract application in terms of survival, mortality, larvae weight and larval stage length. Besides of that, it was the first time this species was recorded in Alagoas state.