Ellen H. Goldberg
University of New Mexico
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Featured researches published by Ellen H. Goldberg.
Archive | 1985
Cory Teuscher; Sm Smith; Ellen H. Goldberg; Gene M. Shearer; Kenneth S. K. Tung
Inbred strains of mice were studied for their susceptibility to the induction of experimental allergic orchitis after sensitization with mouse testicular homogenate in complete Freunds adjuvant accompanied by injections of extract from Bordetella pertussis. Susceptibility to autoimmune orchitis was found to be linked to the major histocompatibility complex in BALB/c and C57BL/10 mice and mapped to genes encoded within the H-2Ddregion. In five of six groups of bidirectional (susceptible × resistant) F1 hybrids, H-2Dd-linked susceptibility was inherited as a dominant autosomal trait. However, in (BALB/cByJ × DBA/2J)F1 and (DBA/2J × BALB/cByJ)F1 hybrids, dominant autosomal resistance to the induction of autoimmune orchitis was observed. Backcross analysis between the resistant F1 hybrid and the susceptible BALB/cByJ parent suggests that a single independently segregating DBA/2J locus is capable of negating H-2Dd-linked susceptibility, and controls resistance to the induction of autoimmune orchitis.
Immunogenetics | 1981
Ellen H. Goldberg; Gideon Goldstein; Edward A. Boyse; Margrit P. Scheid
Although young adult C3H/HeJ (OH) females do not reject C3H male skin grafts, OH females older than 1 year commonly do so, as also do many thymectomized, young adult C3H females. Therapy With TP5, a synthetic pentapeptide analogue of thymopoietin which has biological properties of the parent molecule, substantially reduced the capacity of aged OH females and of thymectomized, young OH females to reject OH male skin.
International Journal of Immunogenetics | 1984
Mark Shapiro; Ellen H. Goldberg
Using a monoclonal antibody to H‐Y antigen we characterized the molecular determinant responsible for H‐Y reactivity. H‐Y‐positive cells were treated with enzymes which alter carbohydrate structure and then were tested for their ability to absorb monoclonal anti‐H‐Y antibody. Indications are that the serological determinant recognized by this antibody is a glycoconjugate containing terminal non‐reducing and internal galactosyl residues.
Journal of Immunological Methods | 1978
Ellen H. Goldberg; Terecita Arrington; Sei Tokuda
Abstract Using the Staphylococcus aureus binding technique, we have demonstrated H-Y (‘male’) antigen on human male leukocytes.
Immunogenetics | 1994
Dianne Rees; Muriel N. Nesbitt; Ellen H. Goldberg
The Skn alloantigens of the mouse are strain-specific proteins confined to cells sharing a common ectodermal lineage (Rees et al. 1993; Jackman et al. 1989: Scheid et al. 1972). Proteins encoded by Skn are expressed in epidermal cells of skin and distinct populations of neural cells of adult mice, yet fail to accumulate in hematopoietic cells. As a consequence, Skn alloantigenic disparities between A/J (A) and C57BL/6 (B6) mice result in a tissue-restricted histoincompatibility between these strains such that lethally irradiated B6 mice reconstituted with spleen cells from (B6 × A) F1 donors (denoted (B6 x A)/B6 chimeras), will inevitably reject skin grafts from A and (B6 x A) F1 mice, while accepting skin grafts from B6 mice (Boyse et al. 1970). Skn alloantigens have been shown to be targets of skin-directed autoimmune responses (Jackman and Goldberg 1993) and are candidates as differentiation antigens directing the commitment of cells to specific cell lineages (Boyse et al. 1970; Rees et al. 1993). Earlier studies by Wachtel and co-workers (1977) in which skin from [(B6 x A) F1 x B6] backcross segregants was grafted to (B6 x A)/B6 chimeras showed that Skn antigens are encoded by two unlinked genes, Skn 1 and Skn 2, each with alternative alleles in A (Skn 1 a, Skn 2a; Skn 1.1 and Skn 2.1 alloantigens) and B6 mice (Skn i b, Skn 2b; Skn 2.1 and Skn 2.1 alloantigens); a disparity at either locus is sufficient to mediate a rejection response. Others, however, have argued that Skn is a genetically complex locus represented by multiple genes (Fleming and Silvers 1981). In order to begin to resolve these issues as well as to provide a basis for characterizing strain-specific polymorphisms in Skn genes, we have initiated a molecular genetic characterization of the Skn histoincompatibility
Immunogenetics | 1990
Ellen H. Goldberg; Robin Goble; Susan H. Jacknan
The purpose of this communication is to describe a second Skn-specific monoclonal antibody, designated SK4, recognizing the Skn 1.1 alloantigen which has been used to provide information regarding the Skn 1.1 allotype of a panel of inbred strains using immunofluorescence and flow cytometric analysis
Journal of Immunological Methods | 1984
Jeanne Monroy Meck; Ellen H. Goldberg
A sensitive and reliable serological assay for the detection of H-Y antigen is described which uses monoclonal H-Y antibody and a cellular radioimmunobinding assay on cultured human fibroblasts. Cell lines from 2 normal human females, 2 normal human males and one XX human male were tested in 3 separate assays. Cells from XY and XX males were found to contain H-Y antigen; however, the reaction against the XX male cells was found to be intermediate between the XY male and normal XX female cells.
Archive | 1987
Ellen H. Goldberg; Brian D. Reilly
The H-Y histocompatibility antigen was originally described as a cell surface component, responsible for the rejection of male skin grafts by otherwise histocompatible females of the same inbred strain1, 2. It seemed reasonable at the time to call the gene responsible for the expression of this male-specific antigen H-Y, because the incompatibility between the male and female had to be somehow linked to the Y chromosome3, 4.
Journal of Immunological Methods | 1991
Brian D. Reilly; Ellen H. Goldberg
An indirect cellular enzyme immunoassay for the detection of the serologically defined male-specific antigen, SDMA, was developed using mouse spermatozoa as the target. Serum from B6 female mice injected with male spleen cells was used as the source of SDMA-specific antibody. Our results indicate that the assay is highly reliable (96% accurate) with an intra- and interassay coefficient of variation less than 12%. Since this assay is non-subjective and simple to perform it provides a useful alternative to the complement-dependent cytotoxicity assay for detection of SDMA.
Journal of Immunological Methods | 1982
Joyce Anglin Shelton; Ellen H. Goldberg
Abstract Skin grafts between inbred strains of mice that differ with respect to an X-linked histocompatibility locus are generally rejected. Using a cellular radioimmunobinding assay, we have serologically demonstrated the presence of an X-associated antigen on lymphocytes of A/J female mice.