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Endocrinology | 1997

Human chorionic gonadotropin-beta gene expression in first trimester placenta.

Amanda K. Miller-Lindholm; C. J. LaBenz; Jacques Ramey; Elliott Bedows; Raymond W. Ruddon

The hCGβ gene family contains six genes linked in tandem on chromosome 19 and labeled β genes 7, 8, 5, 1, 2, and 3. Previous studies on a small number of placentas have indicated that β gene 5 was the most highly expressed gene during the first trimester of pregnancy, followed by genes 3 and 8. β genes 7, 1, and 2 were expressed at very low levels. The purpose of this study was to determine 1) whether this pattern of expression was typical during normal pregnancy by sampling a large number of first trimester placentas, and 2) whether there was a correlation between gestational age and the pattern of hCGβ gene expression. Total RNA from 27 first trimester placentas varying in age from 6–16 weeks was reverse transcribed into complementary DNA. The complementary DNA was amplified by PCR, and the amount of DNA representative of each hCGβ gene was quantified by Genescan analysis. In 14 of the 27 placentas, hCGβ gene 5 accounted for 50% or more of the total β messenger RNA expressed.β gene 3 was expressed at le...


Endocrinology | 1997

Human Chorionic Gonadotropin-β Gene Expression in First Trimester Placenta

Amanda K. Miller-Lindholm; C. J. LaBenz; Jacques Ramey; Elliott Bedows; Raymond W. Ruddon

The hCGβ gene family contains six genes linked in tandem on chromosome 19 and labeled β genes 7, 8, 5, 1, 2, and 3. Previous studies on a small number of placentas have indicated that β gene 5 was the most highly expressed gene during the first trimester of pregnancy, followed by genes 3 and 8. β genes 7, 1, and 2 were expressed at very low levels. The purpose of this study was to determine 1) whether this pattern of expression was typical during normal pregnancy by sampling a large number of first trimester placentas, and 2) whether there was a correlation between gestational age and the pattern of hCGβ gene expression. Total RNA from 27 first trimester placentas varying in age from 6–16 weeks was reverse transcribed into complementary DNA. The complementary DNA was amplified by PCR, and the amount of DNA representative of each hCGβ gene was quantified by Genescan analysis. In 14 of the 27 placentas, hCGβ gene 5 accounted for 50% or more of the total β messenger RNA expressed.β gene 3 was expressed at le...


Biochemical Journal | 1997

TETRAMERIZATION DOMAIN OF HUMAN BUTYRYLCHOLINESTERASE IS AT THE C-TERMINUS

Renee M. Blong; Elliott Bedows; Oksana Lockridge


Protein Science | 1996

Protein folding in the endoplasmic reticulum: lessons from the human chorionic gonadotropin beta subunit.

Raymond W. Ruddon; Simon Sherman; Elliott Bedows


Journal of Biological Chemistry | 1993

Protein folding and assembly in vitro parallel intracellular folding and assembly. Catalysis of folding and assembly of the human chorionic gonadotropin alpha beta dimer by protein disulfide isomerase.

J R Huth; Fulvio Perini; Oksana Lockridge; Elliott Bedows; Raymond W. Ruddon


Journal of Biological Chemistry | 1992

Domain-dependent protein folding is indicated by the intracellular kinetics of disulfide bond formation of human chorionic gonadotropin beta subunit.

J R Huth; K Mountjoy; Fulvio Perini; Elliott Bedows; Raymond W. Ruddon


Journal of Biological Chemistry | 1992

Kinetics of folding and assembly of the human chorionic gonadotropin beta subunit in transfected Chinese hamster ovary cells.

Elliott Bedows; J R Huth; Raymond W. Ruddon


Archive | 2004

Method for purifying a protein of the cystine-knot superfamily

Elliott Bedows; Jason A. Wilken


Journal of Biological Chemistry | 2004

Identification of Disulfide Bonds among the Nine Core 2 N-Acetylglucosaminyltransferase-M Cysteines Conserved in the Mucin β6-N-Acetylglucosaminyltransferase Family

Jaswant Singh; Gausal A. Khan; Leo Kinarsky; Helen Cheng; Jason A. Wilken; Kyung Hyun Choi; Elliott Bedows; Simon Sherman; Pi Wan Cheng


American Journal of Obstetrics and Gynecology | 2003

Maternal circulating bioactive and immunoactive human chorionic gonadotropin in hellp syndrome

Chaur-Dong Hsu; Luis Garcia; Jaqueline Pavlik; Jackson Wilken; Hassan Harirah; Elliott Bedows

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