Eloy Bécares

Long-term operation of high rate algal ponds for the bioremediation of piggery wastewaters at high loading rates.

The performance of two 464-L high rate algal ponds (HRAPs) treating 20- and 10-folds diluted swine manure at 10 days of hydraulic residence time was evaluated under continental climatic conditions in Castilla y Leon (Spain) from January to October. Under optimum environmental conditions (from July to September), both HRAPs supported a stable and efficient carbon and nitrogen oxidation performance, with average COD and TKN removal efficiencies of 76+/-11% and 88+/-6%, respectively, and biomass productivities ranging from 21 to 28 g/m(2)d. Nitrification was identified as the main TKN removal mechanism at dissolved oxygen concentrations higher than 2mg/L (accounting for 80-86% of the TKN removed from January to May and for 54% from July to September). On the other hand, empirical evidences of a simultaneous nitrification-denitrification process were found at dissolved oxygen concentrations lower than 0.5mg/L (high organic loading rates). However, despite the achievement of excellent COD and nitrogen oxidation performance, phosphorous removal efficiencies lower than 10% were recorded in both HRAPs probably due to the high buffer capacity of the piggery wastewater treated (absence of abiotic pH-mediated PO(4)(3-) precipitation). Finally, a detailed monitorization of the dynamics of microalgae population revealed that the combination of moderate temperatures/solar irradiances and high organic loading rates, prevailing during late spring and summer, supported higher microalgae diversities than those found during winter conditions.

State of the art in the functioning of shallow Mediterranean lakes: workshop conclusions

Studies on shallow lakes from the north temperate zone show that they alternate between clear and turbid water states in response to control factors. However, the ecology of semiarid to arid shallow Mediterranean lakes is less explored. Hydrological effects (e.g. water level fluctuations, water residence time) on major ions and nutrient dynamics and processes, and ecology of submerged macrophytes appear to have a crucial role for food webs in shallow Mediterranean lakes. Nutrient control may be of greater priority in eutrophicated warm shallow lakes than in similar lakes at higher latitudes. This will be relevant for the implementation of the European Water Framework Directive, and conservation and management of these ecosystems. Strong trophic cascading effects of fish resulting from dominance of omnivorous and benthivorous fish species, whose diversity is usually high, together with frequent spawning and absence of efficient piscivores, seem to be the reason for the lack of large-bodied grazers that could control phytoplankton. However, such effects may vary within the region depending on fish distribution and community. These factors need elaboration in order to allow shallow lake ecologists and managers to develop better restoration strategies for eutrophicated shallow Mediterranean lakes. Consequently, modifications for the implementation of the European Water Framework Directive for determining ecological status in shallow Mediterranean lakes appear to be necessary. Furthermore, the implications of climate warming may be even more challenging than in high latitude lakes since shallow lakes in the Mediterranean region are among the most sensitive to extreme climate changes. There is an urgent need for data on the ecology of shallow lakes in the region. An appeal is made for international cooperation, development of large-scale research and information exchange to facilitate this and a web-based discussion list has been implemented.

Loss of diversity and degradation of wetlands as a result of introducing exotic crayfish

The introduction of the alocthonous Louisiana red swamp crayfish (Procambarus clarkii) in Chozas (a small shallow lake situated in León (North-West Spain)) in 1996 switched the clear water conditions that harboured an abundant and a quite high richness of plants, invertebrates, amphibians and birds to a turbid one followed by strong losses in abundance and richness in the aforementioned groups. Crayfish exclusion experiments done in Chozas previous to this work confirmed the role of crayfish herbivorism on macrophyte destruction that had a trophic cascade effect on the wetland ecosystem. Direct and indirect effects of crayfish introduction on Chozas lake communities have been evaluated and compared with previous conditions before 1996 or with other related lakes in which crayfish were no present. Crayfish had a main role in submerged plant destruction and a potential effect on amphibia and macroinvertebrate population decrease. Plant destruction (99 % plant coverage reduction) was directly related to invertebrates (71 % losses in macroinvertebrate genera), amphibia (83 % reductions in species), and waterfowls (52 % reduction). Plant-eating birds were negatively affected (75 % losses in ducks species); nevertheless, fish and crayfish eating birds increased their presence since the introduction. Introduction of crayfish in shallow plant-dominated lakes in Spain is a main risk for richness maintenance in these endangered ecosystems.

Comprehensive assessment of the design configuration of constructed wetlands for the removal of pharmaceuticals and personal care products from urban wastewaters

Seven mesocosm-scale constructed wetlands (CWs) of different configurations were operated outdoors for nine months to assess their ability to remove pharmaceuticals and personal care products (PPCPs) from urban wastewaters. CWs differed in some design parameters, namely the presence of plants, the species chosen (i.e., Typha angustifolia vs Phragmites australis), flow configuration (i.e., surface flow vs subsurface flow) and the presence of a gravel bed. A nearby conventional activated-sludge wastewater treatment plant (WWTP) fed with the same sewage was simultaneously monitored for comparison. The PPCPs ketoprofen, naproxen, ibuprofen, diclofenac, salicylic acid, carbamazepine, caffeine, galaxolide, tonalide and methyl dihydrojasmonate were monitored. The presence of plants favoured the removal of some PPCPs. The performance of the mesocosm studied was compound-dependant, soilless CWs showing the highest removal efficiency for ketoprofen, ibuprofen and carbamazepine, while free-water CWs with effluent leaving through the bottom of the tank performed well for the degradation of ketoprofen, salicylic acid, galaxolide and tonalide. Finally, subsurface horizontal flow CWs were efficient for the removal of caffeine. Significant linear correlations were observed between the removal of some PPCPs and temperature or redox potential. Hence, microbiological pathways appear to be the most probable degradation route for PPCPs in the CWs studied.

Assessment of full-scale natural systems for the removal of PPCPs from wastewater in small communities.

This study assessed the ability to remove pharmaceuticals and personal care products (PPCPs) of three different full-scale hybrid pond-constructed wetlands and a conventional wastewater treatment plant (WWTP). The four systems were fed with primary-treated urban wastewaters. The three hybrid systems consisted of several different subsystems (ponds, surface flow constructed wetlands and horizontal subsurface flow constructed wetlands) connected in series, and their PPCP degradation efficiency was monitored. In addition, the enantiomeric behaviour of ibuprofen was studied in all the subsystems. The hybrid systems were at least as efficient in PPCP removal as the WWTP, removal efficiencies mainly exceeding 70%. Moreover, enantiomeric analysis indicates that ibuprofen removal followed a predominantly aerobic and microbiological pathway. Constructed wetlands and ponds are therefore successful technologies for removing PPCPs from wastewater and the most significant removal process in these systems is biologically mediated.

Efficiency of natural systems for removal of bacteria and pathogenic parasites from wastewater

A combined constructed wetland formed by a facultative pond (FP), a surface flow wetland (SF) and a subsurface flow wetland (SSF) was studied from December 2004 until September 2005 in north-western Spain in order to evaluate their efficiency in the removal of pathogenic and indicator microorganisms and to determine their relationships. Microbial removal ranged from 78% for coliphages to over 99% for helminth eggs, depending on the treatment system. The highest removal of indicator bacteria (total coliforms, E. coli, faecal streptococci and Clostridium perfringens) occurred in the stabilization pond, reaching 84%, 96%, 89% and 78%, respectively. However, the greatest removal of protozoan pathogens (Cryptosporidium and Giardia) and coliphages was found in the SSF wetland, 98%, 97% and 94%, respectively. In contrast, the SF wetland was most efficient in the removal of pathogenic parasites when considering superficial removal rates. Seasonal differences in organism removal were not statistically significant during the study period. First-order removal rate constants ranged from 0.0027 to 0.71 m/d depending on the microorganism and type of wetland. Significant correlations were found between pathogenic parasites and faecal indicators in the influent of the treatment system but not in the other sampling points suggesting that such relations varied along the system due to the different survival rates of the microorganisms.

A comparative evaluation of microalgae for the degradation of piggery wastewater under photosynthetic oxygenation

Two green microalgae (Scenedesmus obliquus and Chlorella sorokiniana), one cyanobacterium (Spirulina platensis), one euglenophyt (Euglena viridis) and two microalgae consortia were evaluated for their ability to support carbon, nitrogen and phosphorous removal in symbiosis with activated sludge bacteria during the biodegradation of four and eight times diluted piggery wastewater in batch tests. C. sorokiniana and E. viridis were capable of supporting the biodegradation of four and eight times diluted wastewater. On the other hand, while S. obliquus and the consortia isolated from a swine manure stabilization pond were only able to grow in eight times diluted wastewater, S. platensis and the consortium isolated from a high rate algal pond treating swine manure were totally inhibited regardless of the dilution applied. TOC removal efficiencies (RE) ranging from 42% to 55% and NH(4)(+)-RE from 21% to 39% were recorded in the tests exhibiting photosynthetic oxygenation. The similar oxygen production rates exhibited by the tested microalgae under autotrophic conditions (from 116 to 133mgO(2)L(-1)d(-1)) suggested that factors other than the photosynthetic oxygenation potential governed piggery wastewater biodegradation. Microalgal tolerance towards NH(3) was hypothesized as the key selection criterion. Further studies in a continuous algal-bacterial photobioreactor inoculated with C. sorokiniana, S. obliquus and S. platensis showed that C. sorokiniana, the species showing the highest NH(3)-tolerance, rapidly outcompeted the rest of the microalgae during the biodegradation of eight times diluted wastewater, achieving TOC and NH(4)(+)-RE comparable to those recorded in the batch biodegradation tests.

Coagulation/flocculation-based removal of algal–bacterial biomass from piggery wastewater treatment

Two conventional chemical coagulants (FeCl3 and Fe2(SO4)3) and five commercial polymeric flocculants (Drewfloc 447, Flocudex CS/5000, Flocusol CM/78, Chemifloc CV/300 and Chitosan) were comparatively evaluated for their ability to remove algal-bacterial biomass from the effluent of a photosynthetically oxygenated piggery wastewater biodegradation process. Chlorella sorokiniana, Scenedesmus obliquus, Chlorococcum sp. and a wild type Chlorella, in symbiosis with a bacterial consortium, were used as model algal-bacterial consortia. While the highest biomass removals (66-98%) for the ferric salts were achieved at concentrations of 150-250 mg L(-1), dosages of 25-50 mg L(-1) were required for the polymer flocculants to support comparable removal efficiencies. Process efficiency declined when the polymer flocculant was overdosed. Biomass concentration did not show a significant impact on flocculation within the concentration range tested. The high flocculant requirements herein recorded might be due to the competition of colloidal organic for the flocculants and the stationary phase conditions of biomass.

Shift from clear to turbid phase in Lake Chozas (NW Spain) due to the introduction of American red swamp crayfish (Procambarus clarkii)

Lake Chozas (León, NW Spain) until 1997 was a mesotrophic, macrophyte-dominated clear-water lake. The American red swamp crayfish (Procambarus clarkiiGirard) was introduced in 1995–96. In 1998, it increased its numbers coinciding with reductions in plant density from 97% to less than 10% surface cover. The removal of submerged vegetation was accompanied by a fast switch from clear to turbid, Microcystis-dominated conditions in the lake. In situ experiments in Lake Chozas using exclosures and enclosures have proved that the red crayfish are efficient predators of macrophytes. This work gives new evidence of the direct relationship between red-crayfish introduction and the shift from clear to turbid conditions in shallow lakes.

Removal of antibiotics from urban wastewater by constructed wetland optimization

Seven mesocosm-scale constructed wetlands (CWs), differing in their design characteristics, were set up in the open air to assess their efficiency to remove antibiotics from urban raw wastewater. A conventional wastewater treatment plant (WWTP) was simultaneously monitored. The experiment took place in autumn. An analytical methodology including HPLC-MS/MS was developed to measure antibiotic concentrations in the soluble water fraction, in the suspended solids fraction and in the WWTP sludge. Considering the soluble water fraction, the only easily eliminated antibiotics in the WWTP were doxycycline (61±38%) and sulfamethoxazole (60±26%). All the studied types of CWs were efficient for the removal of sulfamethoxazole (59±30-87±41%), as found in the WWTP, and, in addition, they removed trimethoprim (65±21-96±29%). The elimination of other antibiotics in CWs was limited by the specific system-configuration: amoxicillin (45±15%) was only eliminated by a free-water (FW) subsurface flow (SSF) CW planted with Typha angustifolia; doxycycline was removed in FW systems planted with T. angustifolia (65±34-75±40%), in a Phragmites australis-floating macrophytes system (62±31%) and in conventional horizontal SSF-systems (71±39%); clarithromycin was partially eliminated by an unplanted FW-SSF system (50±18%); erythromycin could only be removed by a P. australis-horizontal SSF system (64±30%); and ampicillin was eliminated by a T. angustifolia-floating macrophytes system (29±4%). Lincomycin was not removed by any of the systems (WWTP or CWs). The presence or absence of plants, the vegetal species (T. angustifolia or P. australis), the flow type and the CW design characteristics regulated the specific removal mechanisms. Therefore, CWs are not an overall solution to remove antibiotics from urban wastewater during cold seasons. However, more studies are needed to assess their ability in warmer periods and to determine the behaviour of full-scale systems.