Elvira Sales-Baptista

Factors Influencing Livestock Productivity

Numerous factors affect livestock production and productivity. In this chapter we will address those that are of paramount importance: climate, nutrition, and health aspects. In initial section we will review the effects of climate on livestock productivity and provide examples of livestock adaptation to climate constraints such as the Bos indicus cattle breeds adapted to hot weather and the fat tailed sheep, particularly adapted to arid conditions. In subsequent sections we address the influence of diseases and parasitism on livestock production and provide specific case studies on how diseases and parasites conditions affect livestock productivity and how domestic animals have adapted in order to cope with them. Finally, we describe two major nutrition-related factors affecting livestock productivity: seasonal weight loss and the browsing vs. grazing abilities in ruminants at the level of the oral cavity. In all section, case studies are provided as examples of specific adaptations to these problems.

The Effect of Tannins on Mediterranean Ruminant Ingestive Behavior: The Role of the Oral Cavity

Sheep, cattle and goat are domestic ruminants of significant economic interest in the Mediterranean region. Although sharing the same pasture ranges, they ingest different plants and plant parts and, consequently different levels of tannins. This suggests an ability to detect and adapt ingestion according to animal physiological limits of tolerance for plant secondary metabolites. This review will detail the effects of dietary tannins on feeding behavior, and the role of the oral cavity in this process, with focus on such ruminant species. The role of salivary protein profile in tannin perception in the oral cavity, and as a defense mechanism, will be discussed.

Assessing foraging strategies of herbivores in Mediterranean oak woodlands: a review of key issues and selected methodologies

Montados are agro-silvo-pastoral ecosystems, typical of the Southwest Iberian Peninsula, of high socio-economic and conservation importance, where grazing is a dominant activity. Montados are characterized by an open tree canopy of Quercus sp. and a diverse undercover of shrubs and grasslands that constitute the plant food resources for grazing animals. Plant food resources of Montados are highly variable, both spatially and seasonally, in quantity and quality. Reliable and easy to use methods to monitor grazing are necessary to allow proper understanding of foraging strategies of grazing animals and to set sustainable grazing management. We describe the main characteristics of the plant food resources available for grazing animals, striking its variability, and revise the potential of using N-alkanes and saliva proteome methods to assess foraging strategies in Montados. In a scenario of dynamic multiple choices, we discuss the use of n-alkane methodology for the simultaneous estimation of diet composition and voluntary intake and saliva proteome as a mean of increasing the knowledge on diet adjustments.

Overgrazing in the Montado? The need for monitoring grazing pressure at paddock scale

AbstractMontados are presently facing the threat of either abandonment or intensification, and livestock overgrazing has been suspected of contributing to reduced natural regeneration and biodiversity. However, reliable data are to our knowledge, lacking. To avoid potential risks of overgrazing, an adaptive and efficient management is essential. In the present paper we review the main sources of complexity for grazing management linked with interactions among pasture, livestock and human decisions. We describe the overgrazing risk in montados and favour grazing pressure over stocking rate, as a key indicator for monitoring changes and support management decisions. We suggest the use of presently available imaging and communication technologies for assessing pasture dynamics and livestock spatial location. This simple and effective tools used for monitoring the grazing pressure, could provide an efficient day-to-day aid for farm managers’ operational use and also for rangeland research through data collection and analysis.

Identification of novel genes for bitter taste receptors in sheep (Ovis aries)

Genetic studies on taste sensitivity, and bitter taste receptors (T2R) in particular, are an essential tool to understand ingestive behavior and its relation to variations of nutritional status occurring in ruminants. In the present study, we conducted a data-mining search to identify T2R candidates in sheep by comparison with the described T2R in cattle and using recently available ovine genome. In sheep, we identified eight orthologs of cattle genes: T2R16, T2R10B, T2R12, T2R3, T2R4, T2R67, T2R13 and T2R5. The in silico predicted genes were then confirmed by PCR and DNA sequencing. The sequencing results showed a 99% to 100% similarity with the in silico predicted sequence. Moreover, we address the chromosomal distribution and compare, in homology and phylogenetic terms, the obtained genes with the known T2R in human, mouse, dog, cattle, horse and pig. The eight novel genes identified map either to ovine chromosome 3 or 4. The phylogenetic data suggest a clustering by receptor type rather than by species for some of the receptors. From the species analyzed, we observed a clear proximity between the two ruminant species, sheep and cattle, in contrast with lower similarities obtained for the comparison of sheep with other mammals. Although further studies are needed to identify the complete T2R repertoire in domestic sheep, our data represent a first step for genetic studies on this field.

Tree influence on soil and pasture: contribution of proximal sensing to pasture productivity and quality estimation in montado ecosystems

ABSTRACT Montado is a silvo-pastoral ecosystem of the Mediterranean region, a mixed system of trees and pasture, subject to animal grazing. Farmers need information on pasture production and quality in order to assess the direct effect of tree presence on the productivity of their pastoral system, and to devise management that balances farm production and profitability with sustainable soil management. The main objectives of this work were (1) to evaluate tree influence on soil and pasture parameters and (2) to evaluate the use of proximal sensing techniques that have potential for monitoring aspects related to spatial and temporal variability of pasture productivity and quality in montado ecosystems. Both objectives can support the decision-making process of the farmer. The study field is located in Mitra farm, in Southern Portugal. During October 2015, 24 geo-referenced composite soil samples (12 under tree canopy and 12 outside tree canopy) were collected from the 0.0–0.3 m soil layer. The soil samples were analysed for texture (sand, silt, and clay content), moisture content, pH, organic matter, total nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg), and manganese (Mn). The evolution of the pasture was recorded in the 24 sampling points at five monitoring dates: at the end of autumn (December 2015), at the end of winter (March 2016), and then monthly during spring 2016 (April, May, and June). The following pasture parameters were measured: normalized difference vegetation index (NDVI), capacitance, temperature, green and dry matter, ash, crude protein (CP), and neutral detergent fibre. Soil under tree canopy had significantly higher levels of organic matter, N, P, K, and Mg, and better pasture quality while the pasture productivity was higher outside tree canopy. The correlation between pasture direct measurements and sensor parameters was more consistent between capacitance and pasture productivity and between NDVI and CP. The use of fast and efficient tools associated with geo-referenced systems can greatly simplify the pasture monitoring process, which is the basis for estimating feed availability in the field. The knowledge of biomass quality and quantity is fundamental to support decision-making regarding animal stocking rates and rotation among grazing parcels.

Sequence Analysis of Bitter Taste Receptor Gene Repertoires in Different Ruminant Species.

Bitter taste has been extensively studied in mammalian species and is associated with sensitivity to toxins and with food choices that avoid dangerous substances in the diet. At the molecular level, bitter compounds are sensed by bitter taste receptor proteins (T2R) present at the surface of taste receptor cells in the gustatory papillae. Our work aims at exploring the phylogenetic relationships of T2R gene sequences within different ruminant species. To accomplish this goal, we gathered a collection of ruminant species with different feeding behaviors and for which no genome data is available: American bison, chamois, elk, European bison, fallow deer, goat, moose, mouflon, muskox, red deer, reindeer and white tailed deer. The herbivores chosen for this study belong to different taxonomic families and habitats, and hence, exhibit distinct foraging behaviors and diet preferences. We describe the first partial repertoires of T2R gene sequences for these species obtained by direct sequencing. We then consider the homology and evolutionary history of these receptors within this ruminant group, and whether it relates to feeding type classification, using MEGA software. Our results suggest that phylogenetic proximity of T2R genes corresponds more to the traditional taxonomic groups of the species rather than reflecting a categorization by feeding strategy.

Progress in Identifying High Nature Value Montados: Impacts of Grazing on Hardwood Rangeland Biodiversity

ABSTRACT Due to their complex structure and traditional low-intensity management, Portuguese oak woodland rangelands known as montados are often considered high nature value (HNV) farming systems, and as such, they may be deemed eligible for subsidies and incentives by governmental and nongovernmental agencies. Too little is known about how the HNV concept might be applied to conserve complex silvopastoral systems. These systems, due to their structural and functional complexity at multiple scales, tend to support high levels of biodiversity. Montados are in sharp decline as a result of the rapid specialization of land management that, through simplification, undermines multifunctionality. Understanding how changes in management influence these systems and their biodiversity is needed for prioritizing conservation efforts and for ensuring they remain HNV systems. On the basis of a field survey in 58 plots distributed among 29 paddocks on 17 farms, we conducted an integrated analysis of the relationship between grazing intensity and biodiversity in montados of similar biophysical and structural characteristics. Data on management were obtained through interviews, and biodiversity data (vegetation, macrofungi, birds, herpetofauna) were obtained through specific field protocols. Additional spatial data, such as soil characteristics, slope, land cover, and linear landscape elements, were also analyzed. The results show no overall biodiversity variation as a result of different management practices. However, different groups of species react differently to specific management practices, and within a pasture, grazing impacts are heterogenous. In low grazing intensity plots, macrofungi species richness was found to be higher, while bird species richness was lower. Using tree regeneration as proxy for montado sustainability, results show less tree regeneration in areas with higher forage quality and more intense grazing. Pathways for future progress are proposed, including creating areas within a paddock that attract grazing away from where regeneration is desired.

Cattle-driven forest disturbances impact ensemble composition and activity levels of insectivorous bats in Mediterranean wood pastures

Abstract Forests can be modified by fine-scale disturbances such as those prompted by cattle grazing, but their impacts on biodiversity are far from being understood. Here, we investigate the response of insectivorous bats to cattle-driven forest disturbances, using a savanna-like Mediterranean agroforestry system, the Portuguese montado, as study system. In doing so, we compared bat ensemble composition and activity levels of bats across sites that differed in how frequently they were used by free-ranging cattle. Specifically, we selected sites regularly used by cattle (central places) and sites seldom used (grazing sites). We found strong between-site differences in both bat species composition and activity levels, with lower diversity and activity in central places compared to grazing sites. These response patterns, corresponded to marked between-site differences in tree cover, seemingly driven by cattle use given the lower levels of tree regeneration and vegetation productivity in central places compared to grazing sites. Our work therefore demonstrates that it is not only severe forest loss and fragmentation that has an impact on insectivorous bats, but also when these processes operate at fine spatial scales. We thus suggest that fine-scale forest disturbances, particularly those driven by recurrent cattle use, cannot be neglected in the conservation management of agroforestry systems. In this context, we propose some management strategies aimed at counteracting the impact of cattle-driven disturbances on biodiversity in general, and on insectivorous bats in particular.

Identification of a Bitter-Taste Receptor Gene Repertoire in Different Lagomorphs Species

The repertoires of bitter-taste receptor (T2R) gene have been described for several animal species, but these data are still scarce for Lagomorphs. The aim of the present work is to identify potential repertoires of T2R in several Lagomorph species, covering a wide geographical distribution. We studied these genes in Lepus timidus, L. europaeus, Oryctolagus cuniculus algirus, Romerolagus diazi, and Sylvilagus floridanus, using O. cuniculus cuniculus as control species for PCR and DNA sequencing. We studied the identities of the DNA sequences and built the corresponding phylogenetic tree. Sequencing was successful for both subspecies of O. cuniculus for all T2R genes studied, for five genes in Lepus, and for three genes in R. diazi and S. floridanus. We describe for the first time the partial repertoires of T2R genes for Lagomorphs species, other than the common rabbit. Our phylogenetic analyses indicate that sequence proximity levels follow the established taxonomic classification.