Elżbieta Lonc

Evaluation of Three Microbial Formulations Against Culex pipiens pipiens Larvae in Irrigation Fields in Wroclaw, Poland

Abstract Two evaluations of microbial mosquito larvicides were conducted in irrigation fields located along the Odra River near the city of Wroclaw, Poland. In the first evaluation, 3 dosages each of Bacillus thuringiensis israelensis (Bti)—VectoBac® water dispersible granule (WDG) and VectoBac aqueous suspension formulation (12AS)—and Bacillus sphaericus (Bs)—VectoLex® WDG—were tested against larvae of Culex pipiens pipiens. There was little difference between formulations and rates of application between day 2 and 7 following the treatment. VectoBac WDG at 300 g/ha resulted in 100% mortality of Cx. p. pipiens. Between 0.5 and 1 liter of VectoBac 12AS caused sufficient mortality for up to 7 days. The effective short-term application rate of VectoLex WDG was found to be 400 g/ha. The above doses can be recommended for up to 7-day control in this habitat. In the second evaluation, using VectoBac WDG at 800 g/ha, the residual efficacies of 2 ground application methods, a knapsack sprayer configured for large droplet application and a motorized backpack blower configured for small droplet application, were compared. The results indicated no significant difference between the 2 application methods, but a significant long-term effect of VectoBac WDG was observed at the rate of 800 g/ha.

The role of domestic dogs and cats in the zoonotic cycles of ticks and pathogens. Preliminary studies in the Wroclaw Agglomeration (SW Poland).

The collection of 729 tick specimens (Ixodes ricinus, 88.6%; Ixodes hexagonus, 9.2%; Dermacentor reticulatus, 2.2%) removed from 373 dogs and 78 cats, along with 201 ticks from vegetation (I. ricinus, 75.6%; D. reticulatus, 24.4%), allows one to say that pets play an important role in maintaining tick life cycles in different urban area. It shows the lack of statistical differences between tick intensity in high-impact anthropogenic areas (HIAA), low-impact anthropogenic areas (LIAA) and mixed areas designed, in an objective way, by GIS techniques. The comparable (statistically insignificant) level of infection with Borrelia spp. of I. ricinus from pets (22.5%) and vegetation (24.8%), shows that dogs and cats do not have zooprophylactic competence for Borrelia spp. in different urban areas. Moreover, Borrelia spp. was detected in I. hexagonues (1.5%) collected from pets, and in D. reticulatus (2%) obtained from vegetation. The presence of D. reticulatus in the Wrocław Agglomeration confirms its expansion and the distribution range in Poland.

Molecular identification and biodiversity of potential allergenic molds (Aspergillus and Penicilium) in the poultry house: first report

The production of poultry has proved to be a significant source of fungi. Penicillium and Aspergillus are among the most important species, because they can cause irritations, infections, allergies, spoilage of food and beverages and are able to produce dangerous mycotoxins. Detection and identification of the species in these genera is very significant, because it provides relevant information about the properties of the responsible strains. Therefore, their taxonomic was determined using a phenotypic and molecular (ITS and partial β-tubulin sequences) methods. Investigations were conducted in the poultry house located near Wrocław in Lower Silesia (Poland). Using morphological analyses, 215 fungal strains were identified. Among them, 56 belonged to the Penicilium and Aspergillus genera. The results obtained from sequence analysis corresponded well with the morphological identification. Classification at species level was possible in most cases using the sequence data. Most isolates belong to Penicilium genus, with the follow dominating species (P. chrysogenum, P. polonicum and P. olsonii).

Winter activity of Dermacentor reticulatus (Fabricius, 1794) in the newly emerging population of Lower Silesia, south-west Poland.

This paper presents the unexpected winter activity of Dermacentor reticulatus (Fabricius, 1794) ticks in a newly emerging population in south-west Poland. Host-seeking ticks were collected from vegetation in January 2016 in a meadow ecosystem in six sites located in the Wroclaw Agglomeration, as well as from ten companion animals. A total of 238 questing D. reticulatus ticks, comprising 166 females and 72 males, were collected from all examined sites with the highest number of 102 specimens collected in one hour in one locality (Muchobór Wielki, Wrocław). Additionally, two fully-engorged females were collected from two dogs along with one slightly engorged female from a cat. The fact that D. reticulatus can be very active in January indicates a need to take into account the increased threat of tick-bite in the winter time.