Emel Guney

Cell death-inducing effect of novel palladium(II) and platinum(II) complexes on non-small cell lung cancer cells in vitro

PurposeTreatment for lung cancer is still far from satisfying rates. Therefore, there is a need for novel anticancer agents. For this purpose, novel platinum and palladium complexes {[Pd(sac)(terpy)](sac)·4H2O (Complex 1), [Pt(sac)(terpy)](sac)·5H2O (Complex 2), [PdCl(terpy)](sac)·2H2O (Complex 3), [PtCl(terpy)](sac)·2H2O (Complex 4)} have been tested against three different non-small cell lung cancer cell lines (A549, H1299, PC-3).MethodsGrowth-inhibiting effects have been tested by the MTT and ATP viability assays. Apoptosis has been detected by the caspase-cleaved cytokeratin 18 (M30-antigen) assay. Necrosis has been detected by staining the cells with fluorescent dyes. Mitotic index has been calculated by counting the mitotic figures after staining with hematoxylin.ResultsThe complex 3 exhibited significant anti-growth effects, and its anti-growth effect was more powerful than that of cisplatin that is a standard chemotherapeutic agent for this type of cancer. The complexes did not induce apoptosis, while necrosis clearly took place.ConclusionsNovel Pd(II) complex ([PdCl(terpy)](sac)·2H2O) seems to represent a potentially active drug against non-small cell lung cancer cell lines, and further studies in vivo are warranted.

Synthesis, structural characterization and cell death-inducing effect of novel palladium(II) and platinum(II) saccharinate complexes with 2-(hydroxymethyl)pyridine and 2-(2-hydroxyethyl)pyridine on cancer cells in vitro.

Four palladium(II) and platinum(II) saccharinate (sac) complexes with 2-(hydroxymethyl)pyridine (2-hmpy) and 2-(2-hydroxyethyl)pyridine (2-hepy), namely trans-[Pd(2-hmpy)2(sac)2]·H2O (1), trans-[Pt(2-hmpy)2(sac)2]·3H2O (2), trans-[Pd(2-hepy)2(sac)2] (3) and trans-[Pt(2-hepy)2(sac)2] (4), have been synthesized and characterized by elemental analysis, UV-vis, IR and NMR. Single crystal X-ray analysis reveals that the metal(II) ions in each complex are coordinated by two sac and two 2-hmpy or 2-hepy ligands with a trans arrangement. Anticancer effects of 1-4 were tested against four different cancer cell lines (A549 and PC3 for lung cancer, C6 for glioblastoma, and Hep3B for liver cancer). Cytotoxicity was first screened by the MTT assay and the results were further confirmed by the ATP assay. The mode of cell death was determined by both histological and biochemical methods. Among the metal complexes, complex 2 resulted in relatively stronger anti-growth effect in a dose-dependent manner (3.13-200μM), compared to the others, by inducing apoptosis.

Synthesis, experimental and theoretical characterization of palladium(II) and platinum(II) saccharinate complexes with 2-(2-pyridyl)benzimidazole

New palladium(II) and platinum(II) complexes of saccharinate (sac) with 2-(2-pyridyl)benzimidazole (pybim) have been synthesized and characterized by elemental analysis and spectroscopic techniques. From the experimental studies, these complexes were formulated as [Pd(pybim)(sac)2] (1), and [Pt(pybim)(sac)2]·4H2O (2). The ground-state geometries of both complexes were optimized using density functional theory (DFT) methods at the B3LYP level. A bidentate pybim ligand together with two N-coordinated sac ligands form the square-planar MN4 coordination geometry around the palladium(II) and platinum(II) ions. The calculated IR and UV-vis spectral data have been correlated to the experimental results. Thermal analysis data support the molecular structures of both complexes.

Syntheses, crystal structures, fluorescent, and thermal properties of nickel(II) 5,5-diethylbarbiturate complexes with (2-aminomethyl), (2-aminoethyl), and (2-hydroxyethyl)pyridines

Three nickel(II) complexes of 5,5-diethlybarbiturate (barb) with (2-aminomethyl)pyridine (ampy), (2-aminoethyl)pyridine (aepy), and (2-hydroxyethyl)pyridine (hepy), [Ni(barb-N)2(ampy)2] (1), [Ni(barb-N,O)(aepy)2](barb) · H2O (2), and [Ni(barb-O) 2(hepy)] · 2H2O (3), have been synthesized and characterized by elemental analysis, IR, thermal analysis, and single crystal X-ray diffraction. All complexes are mononuclear with nickel(II) exhibiting an octahedral coordination. Ampy, aepy, and hepy are bidentate chelating ligands, while barb shows different coordination modes. In 1, two barb ligands are N-bonded, whereas in 3 they are O -coordinated through one carbonyl oxygen. In 2, one barb is bidentate chelating, while the second barb remains outside the coordination sphere as a counterion. The molecules of 1–3 are bridged by multiple hydrogen bonds to generate 1-D or 2-D supramolecular networks. All complexes are fluorescent due to π–π* transitions.