Emil Anton

Increased oxidative stress status in rat serum after five minutes treadmill exercise

Although it is accepted that an important correlation exists between the physical exercise and the oxidative stress status, the data regarding the levels of the main oxidative stress markers after physical training have been difficult to interpret and a subject of many controversies. There are also very few studies regarding the effects of short-time exercise on the oxidative stress status modifications. Thus, in the present report we were interested in studying the modifications of some oxidative stress markers (two antioxidant enzymes-superoxide dismutase and glutathione peroxidase, a lipid peroxidation parameter — malondyaldehide, the total antioxidant status and protein carbonyl levels), from the serum of rats that were subject to one bout of five minutes exercise on a treadmill, when compared to a control sedentary group. In this way, we observed a decrease of superoxide dismutase specific activity in the rats which performed the exercises. Still, no modifications of glutathione peroxidase specific activity were found between groups. In addition, increased levels of malondyaldehide and protein carbonyls were observed in the rats subjected to exercises. In conclusion, our data provides new evidence regarding the increase of the oxidative stress status, as a result of a 5-minutes bout of treadmill exercising in rats, expressed through a decrease in the SOD specific activity and the total antioxidant status and also an increase of the lipid peroxidation and protein oxidation processes.

Spontaneous pregnancy after laparoscopic cystectomy

Most women will experience a cyst on the ovaries at least once, and most are painless, cause no symptoms, and are discovered during a routine pelvic exam. Large cysts that can cause symptomatology or infertility problems occur in about 8% among women of reproductive age. The current case report comes to show that laparoscopic surgery is the key for persistent organic tumors of the ovary, after a complete diagnostic of the cyst; not the expectant management or hormonal therapy, but laparoscopic cystectomy after transvaginal punction and drainage of the fluid is minimal requiring as recovery timing, medical care period, low costs, and not the least, ovarian functionality, after suppressing ovulatory function.

THE IMPORTANCE OF CLINICAL AND INSTRUMENTAL DIAGNOSTIC IN THE MAMMARY GLAND CANCER.

T aims of the present proposal are the preparation and utilization nanomaterials to develop a new brain tumor theranostic system by taking advantages of nanotechnology to efficiently deliver Temozolomide (TMZ) and photosensitizer (PpIX) into brain parenchyma and to estimate the feasibility in clinical application. In this study, we will develop targeted core/shell liposomes which can cross the blood brain barrier (BBB) or blood tumor barrier (BTB) and deliver TMZ and PpIX into tumor cells. Besides, we also will irradiate the PpIX by Blue Light Emitter to monitor the location of tumor cells that can enhance the accuracy of surgery, and then the residual tumor cells can be inhibited by targeted chemotherapy and photodynamic therapy. To accomplish this goal, the transferrin receptor antibody (anti-TfR) must be conjugated on the surface of liposomes to significantly raise the its efficacy of BBB penetration. Such as synergistic applications of emerging technologies promise to provide more effective methods of tumor treatment and accurate diagnosis, with lower therapeutic doses and potentially fewer side effects.T with our partner company EPO we have generated patient-derived 3D (PD3DTM) cell culture models and corresponding xenografts from more than 120 patients with primary or metastatic cancers of various origins. Although early diagnosis and molecular characterization of cancer has improved significantly, methods for rapid and cost-effective prediction of an optimal and individualized treatment are still missed. We present an experimental pipeline combining molecular genotyping and experimental drug testing for the individual patient. Starting from Matrigel-based Patient-derived three-dimensional PD3DTM cell cultures, we were able to establish numerous long-term PD3DTM cell cultures suitable as models for basic and translational research. Immunohistochemistry analyses demonstrated, that our in vitro cultures preserve an in vivo-like architecture, preventing tumor cells from differentiating and allowing the investigation of intra-tumor heterogeneity and cancer stem cell-like sub-populations. To interrogate the mutation status of selected clinically relevant oncogenes and tumor suppressors in PD3DTM cultures, we applied cost-efficient benchtop sequencing and show the preservation of putative driver mutations found in the original tumor. IC50 data generated by automated 384-well based dose-response experiments with approved drugs are then used to link individual genotypes with drug sensitivity phenotypes. These drug sensitivity profiles serve as a source of comparison and complementation to drug response data of the corresponding in vivo PDX models or even patients, where applicable. These wet-lab data, combined with in vivo and clinical data, will serve as a basis for both, early phase drug development including companion diagnostics to new predictors for tailored therapies.Breast cancer is the most common oncology disease in women and is one of the major public health issues. Worldwide, is the second leading cause of cancer death in women and cancer research is a priority in all the laboratories of the world, in terms of uncovering the appearance causes of the malignant process, understanding the mechanisms of development, but most of all, the discovery of early diagnostic methods and effective treatment. Ignorance, fear of diagnosis, lack of health education and of efficient programmes for prevention and screening could cause diagnosis of the disease to be detected in the majority of cases in advanced stages, when treatment remains only palliative and very costly, in this cases the patients suffering being immense. In this way, regarding the clinical diagnosis in stage I mammary gland cancer, in the 496 stage I MGC patients, during the primary clinical investigation the diagnosis of stage I MGC was established only in 165 (33.3%) patients, and in 232 (46,8%) patients the diagnosis of suspicion MGC was obtained. Also, in terms of instrumental diagnosis, such as mammography, ultrasonography in mammary gland cancer stage I, it seems that in accordance with literature data the pathological process features assessment in the mammary gland is problematic especially in young age. Thus, it seems that MGC represents a polymorphic and pathogenic disease and it cannot be admitted that all subgroups of patients will obtain identical results from one tactic of treatment determined for all the patients with MGC. In this way, the concept of MGC both clinical and patho morphological, combines different cell clones depending on its microstructure and biology. As a result, the evolution of the disease, the prognosis and the effectiveness of the treatment may vary in different patients at the same stage, depending on the degree of malignancy of the tumor, its histopathological structure, the degree of expression of molecular markers identification and also immune resistance.P Integrated Therapy (PSIT) is a psychosocial and psycho-educational intervention. In this study, it was used to support breast cancer survivors to learn to acknowledge and work with paradoxes (areas of ambiguity, inconsistency and contradiction), which would help them understand, cope with, and transcend the role of cancer in their lives. Previous research established the presence of paradoxes in European Caucasian cancer survivors, but the role of paradoxes among culturally diverse breast cancer survivors, or among participants in an intervention such as PSIT, was not examined. This study examined the experience of 12 breast cancer survivors (6 culturally diverse/6 European Caucasian) who participated in an 8-week PSIT intervention and asked 3 specific questions. First, which of the paradoxes identified in previous research were experienced by breast cancer survivors in the current study? Second, what new paradoxes emerged from the 12 participants’ responses? Third, how did culturally diverse women expand the range of paradoxes in the PSIT intervention? This third question aimed to contribute to an under-researched area: culturally diverse breast cancer survivors. The study used a qualitative methodology, based on a thematic analysis of 12 participant interviews in order to identify paradox themes among breast cancer survivors. Findings included the following: (a) the PSIT group replicated 3 paradoxes named in a previous study by Halstead and Hull; (b) that the PSIT participants developed the ability to work with each side of a paradox and hold interdependent opposing realities together simultaneously, while extending the range of paradoxes to include themes of interconnection, personal empowerment, and spiritual edges and tensions; (c) that culturally diverse PSIT participants expanded the range of paradoxes. They built an awareness, not only of the contradictory and co-existing elements of the paradoxes, but also of how these elements could work together to create balance and deeper integration. Culturally diverse participants searched for authentic spiritual experiences, which included self-transcendence during or after the integration of paradoxes. They contributed to subthemes that expressed the value of their insight gained from witness consciousness (neutral-observer stance), their experiences of moderating control and surrender, and their movement from self-criticism to self-understanding.P cell death (PCD), an active process that leads to cell suicide, is a critical mechanism in eukaryotes and prokaryotes. In bacteria, MazEF is the best studied bacterial PCD system. This system includes a ribonuclease, called MazF, that cleaves mRNAs at ACA sequences leading to inhibition of protein synthesis. This protein is also able to induce Bak-dependent apoptosis in mammalian cells. Colorectal cancer is the second cause of cancer related mortality in the United States. In the present study, the ACA-less mazF gene was inserted into pSF-T7-EMCV T7 IRES expression plasmid. The mRNAs of mazF gene were synthesised in vitro condition and 1 μg/ml of the mRNA was transferred into HT29 (Colorectal adenocarcinoma) cell lines. The incidence of apoptosis was detected by conducting TUNEL and Annexin-V assays. The results showed thatonly 26% of cells when treated with 1μg/mlof mRNA remained attached and 32% out of the attached cells were either in early or late stages of apoptosis after 48 hours. The results suggest that MazF protein could be a suitable apoptotic inducer against cancer cells. Since this protein is able to cleave mRNAs at ACA sites, it could find various targets such as XIAP mRNA in cancer cells for effective control of cancer proliferation. This finding is the first report of the application of MazF as an anti-cancer agent for the induction of apoptosis in HT29 cell lines.I quantity of circulating cell-free DNA (cfDNA) has been shown to correspond to many solid tumors. Circulating cell-free DNA is elevated in patients with renal cell carcinoma (RCC), and limited data suggests potential utilityof cfDNA quantificationin RCC prognosis. While there are select studies analyzing hypermethylation or microsatellite alterations of cfDNA, many studies in RCC analyze total amount and/or length of cfDNA.As there are other non-malignant causes of elevated cfDNA, including endothelial dysfunction, trauma, and autoimmune disease, a more specific method to detect RCCassociated cfDNA would be advantageous.L it is generally believed that the incidence of pancreatic neoplasm has increased exponentially all over the world, and especially in the last decade, by even becoming the fifth cause of death by cancer. However, the vast majority of the patients present to the clinic in the advanced unresectable stages, while significant risk factors for pancreatic cancer are still insufficiently studied. In addition, when it comes to the recurrence of the pancreatic cancer, there are also many unknowns regarding the mechanisms and the factors which are implicated. In this way, in this report we want it to make a concise description and analysis for the relevance of some molecular factors in recurrent pancreatic cancer, by mainly focusing on CA19-9 and cytokeratins, besides the other classical clinical factors which are relevant in the diagnostic of the pancreatic cancer recurrence.T reality of oncologic illnesses obliges those of us in healthcare to consider the paradox of living life while preparing for death or conversely living when the struggles make the thought of death come as a welcomed guest. It is within this chaos of illness where the rhythm of the universe lies. This is the state of the sacred that leads to an expanding awareness of healing and wholeness. The patient and the healthcare practitioner join in a story of each other—we become parts of each other’s story. By fostering the richness and depth of stories and by looking beyond the lens of nursing and medicine this project uses storytelling to form a picture of how the needs, and ultimately the decisions the individual makes, shape the patterns of the group. Likewise, story telling is a way to learn of the “problematics” that too often go “un-narrated. When we begin to hear through the filter of cultural conformity and the social structures which embrace—or constrain—the person living with cancer we create new ways of healing. This project explores the emic wisdom of the storyteller and can be used in practice and in teaching. Acknowledging that when one steps beyond the boundaries of our profession we can explore the needs of populations more fully. In practice, it is a therapeutic model to create a sacred space to heal. As a teaching model it is used as a foundation to educate students about all ways of knowing.I drug uptake by solid tumors remains the major problem for systemic chemotherapy. Although anticancer drug effects are dose-dependent, dose-escalationhas resulted in limited survival benefit with increased toxicities. Loco-regional treatments, offering dramatically higher drug concentrations in tumor tissues while minimizing systemic toxicity, thought to be a solution, but survival benefits are still not sufficient. We have proposed that the main obstacle preventing local drug extraction by the tumor cells is the hydrophilic nature of the drug formulations themselves, which prevents partitioning through membrane lipid bilayer. To overcome this obstacle we have developed an approach for drug hydrophobization in which the drug is linked to a hydrophobic moiety by highly labile chemical linkages [termed rapidly reversible hydrophobization (RRH)]:I modulation in the glioma microenvironment, which can play a pivotal role for outcomes of autologous DC-tumor vaccine adjuvant therapy (ADCTA) of glioblastoma multiforme (GBM). Our clinical investigation had been completed clinical studies of ADCTA Phase II trials in GBM patients, which can effectively prolong survivals via generating anti-tumor immune-activities by ADCTA. However, some GBM patients in the ADCTA trial represented strong immunosuppressive responses, which are considered as a key obstacle for the immunotherapeutic strategy. GBM-mediated immunomodulation is thought to be regulated by various immune factors, such as IL-10, TGF-β. Recently, glioblastoma stem cell (GSC) has been shown abilities to impair immune responses, modulate the microenvironment and differentiate into tumor-derived endothelial cells. In the present study, we found that Foxp3 could play a critical role in GBM cells. We had established the experimental foundation for this approach and enable a functional test of tumor-associated Foxp3 that may influence the activities of metastasis and/or tumor immunosuppression in GBM cells. High level of FOXP3 expression in tissues of GBM patients was exhibited a decreasing trend of survival. Primary GSCs were characterized into two subtypes, PN-GSC (CD133+) and MESGSC (CD133-). In tumor-sphere cultures, FOXP3 was induced and expressed higher in PN-GSC than in MES-GSC.