Emilie Balsemin

Parental participation in progeny and effective population sizes in experimental seed orchards of wild cherry Prunus avium L. (Batsch)

Parents and progenies genetic diversity, and male and female contributions to the seed crop were assessed in three experimental Prunus avium seed orchards. Collected data were used to compare different effective population sizes, based on phenological, seed crop and paternity analysis. Our results did not show any difference of genetic diversity between parents and progenies. A limited pollen pollution was detected. We showed that distance and coflowering among clones had a significant effect on effective pollination, and a significant effect of the production of flowers was revealed in one of the seed orchards. Our study also revealed a quite low number of effective size of fathers per mother, but high effective sizes of mothers, fathers and parents at the level of the seed orchard. Finally, the calculation of effective size of mothers, fathers and parents was not highly modified when having the complete information based on the paternity analysis.RésuméLa diversité génétique des parents et de leurs descendants, ainsi que les contributions mâle et femelle à la récolte de graines ont été estimées dans trois vergers à graines expérimentaux de Prunus avium. Les données collectées ont été utilisées pour comparer différentes tailles de population efficace, en se basant sur l’analyse de la phénologie, de la récolte de graines et de la paternité. Nos résultats n’ont pas montré de différence de diversité génétique entre les parents et les descendants. Une pollution pollinique limitée a été mise en évidence. Nous avons montré un effet significatif de la distance et de la cofloraison entre clones sur la pollinisation efficace, ainsi qu’un effet significatif de la production de fleurs dans l’un des vergers à graines. Notre étude a aussi révélé un faible nombre efficace de pères à l’échelle de chaque mère, et un nombre efficace de mères, de pères et de parents important à l’échelle du verger à graines. Enfin, le calcul des tailles efficaces de pères et de parents à l’échelle du verger à graines n’a pas été fortement influencé par le fait d’avoir l’information totale de contribution donnée par l’analyse de paternité.

Integration of cryopreservation in French plant genetic resource collections: the CRYOVEG project

This book represents contributions, oral as well as posters, of the final meeting of COST Action 871, CRYOPLANET (Cryopreservation of crop species in Europe) held in Angers. Local organizers of the meeting were Dr. Agnes Grapin (AGROCAMPUS OUEST – Angers) and Dr. Florent Engelmann (Institut de Recherche pour le Developpement). COST Action 871 started in December 2006 with a Kick-off meeting at the COST office in Brussels and officially ended in December 2010. Twenty-one COST Action Countries (see figure 1) and 3 non-COST institutes (New Zealand Institute for Crop & Food Research (New Zealand), Vavilov Institute of Plant Industry (Russian Federation), Faculte des Sciences de Sfax (Tunisia) participated actively in this initiative. The Action was created because plant cryobiologists realized that plant cryopreservation was hardly applied in Europe. This was mainly due to the fact that efficient and robust cryopreservation protocols applicable to many plant species and diverse germplasm types were not available, plant researchers were unacquainted to recent developments in cryogenic storage methods and there was a lack of coordinated research in Europe on plant cryopreservation. The main objective of this action was therefore “to improve and apply technologically advanced techniques for plant genetic resources conservation of crops that are grown/ and or conserved in Europe with main emphasis on long-term conservation through cryopreservation”. In order to achieve this, 2 working groups (WGs) were established