Emily D. Matys

Possible early foraminiferans in post-Sturtian (716–635 Ma) cap carbonates

Foraminifera are an ecologically important group of modern heterotrophic amoeboid eukaryotes whose naked and testate ancestors are thought to have evolved ∼1 Ga ago. However, the single-chambered agglutinated tests of these protists appear in the fossil record only after ca. 580 Ma, coinciding with the appearance of macroscopic and mineralized animals. Here we report the discovery of small, slender tubular microfossils in the Sturtian (ca. 716–635 Ma) cap carbonate of the Rasthof Formation in Namibia. The tubes are 200–1300 μm long and 20–70 μm wide, and preserve apertures and variably wide lumens, folds, constrictions, and ridges. Their sometimes flexible walls are composed of carbonaceous material and detrital minerals. This combination of morphologic and compositional characters is also present in some species of modern single-chambered agglutinated tubular foraminiferans, and is not found in other agglutinated eukaryotes. The preservation of possible early Foraminifera in the carbonate rocks deposited in the immediate aftermath of Sturtian low-latitude glaciation indicates that various morphologically modern protists thrived in microbially dominated ecosystems, and contributed to the cycling of carbon in Neoproterozoic oceans much before the rise of complex animals.

Putative Cryogenian ciliates from Mongolia

Major lineages of modern eukaryotes, represented primarily by microscopic taxa, are thought to have originated during the Neoproterozoic, but microfossils older than 635 Ma rarely have unambiguous relationships to modern microscopic eukaryotes. Here we report exceptionally preserved 715–635 m.y. old eukaryotic tests in limestone strata of Mongolia. The ∼100-μm-long organic-rich three-dimensional tests have flask-like shapes, constricted necks, distinct and often thickened collars, and flexible walls composed of densely packed alveolar structures. The combined morphological and ultrastructural characters of these Cryogenian tests are remarkably similar to the tests of tintinnids, modern planktonic ciliates. Eukaryotes forming recalcitrant organic or mineral-rich tests before 635 Ma may have increased export and burial fraction of organic carbon, driving an increase in atmospheric oxygen and the subsequent radiation of metazoans.

FOSSILS OF PUTATIVE MARINE ALGAE FROM THE CRYOGENIAN GLACIAL INTERLUDE OF MONGOLIA

ABSTRACT Neoproterozoic carbonate successions provide a new taphonomic window into the diversification of eukaryotes. We report recently discovered macroscopic organic warty sheets (MOWS) in macerates of limestone from the ca. 662–635 Ma Taishir Formation (Tsagaan Olom Group, Mongolia). Sheets are applanate. One surface contains raised ridges and conspicuous, ∼ 100-µm-tall warty protuberances with depressed tops that enclose internal cavities containing cellular structures. The Taishir MOWS may be the remains of unusual bacterial, protistan, or fungal biofilms, or a previously undocumented, extinct taxon. However, multiple lines of evidence including the morphology of warty protuberances and the presence of cellular architecture within protuberances support the interpretation of MOWS as marine algae, perhaps a member of the Rhodophyta. Regardless of their specific taxonomic affiliation, MOWS increase the diversity of biota reported from the Cryogenian glacial interlude and indicate the presence of macroscopic and morphologically complex multicellular organisms in the Cryogenian.

Raman spectroscopic analysis of carbonaceous matter and silica in the test walls of recent and fossil agglutinated foraminifera

Raman spectroscopy has been used extensively in thermal maturation studies of kerogen, but has not been used to examine the maturation of organic cements in agglutinated foraminifera. Here, we use Raman spectroscopy to document the existence of carbonaceous matter and silica in recent and fossil agglutinated foraminifera, and to measure thermal alteration effects in fossil foraminifera. The distribution of carbonaceous matter through the test (shell) walls of agglutinated foraminifera suggests that this carbonaceous material is derived from primary organic cement and not from random contamination. Fossil specimens exhibit three broad stages of maturation: (1) Immature specimens are characterized by moderately strong fluorescence, broad, low intensity Raman peaks (relative to fluorescence), and a tendency for the G-band to occur at lower wave numbers. These attributes are consistent with the presence of amorphous carbonaceous matter and minor organic degradation. (2) Mature samples (oil window) exhibit high fluorescence, increased relative D- and G-band intensities, and a decreased width of the D-band. (3) Postmature samples exhibit low levels of fluorescence and high relative D- and G-band intensities, a tendency for the G-band to be located at higher wave numbers, an increase in the D:G band ratio, and an increase of the relative intensity of the silica peak. This stage is consistent with the presence of highly ordered carbonaceous matter and diagenetic quartz. These findings indicate that Raman spectroscopic analysis of fossil agglutinated foraminifera can be used as a quick and easy tool to assess thermal maturity and estimate optimal temperatures for hydrocarbon generation.

Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment

The Herman Pit, once a mercury mine, is an impoundment located in an active geothermal area. Its acidic waters are permeated by hundreds of gas seeps. One seep was sampled and found to be composed of mostly CO2 with some CH4 present. The δ13CH4 value suggested a complex origin for the methane: i.e., a thermogenic component plus a biological methanogenic portion. The relatively 12C-enriched CO2 suggested a reworking of the ebullitive methane by methanotrophic bacteria. Therefore, we tested bottom sediments for their ability to consume methane by conducting aerobic incubations of slurried materials. Methane was removed from the headspace of live slurries, and subsequent additions of methane resulted in faster removal rates. This activity could be transferred to an artificial, acidic medium, indicating the presence of acidophilic or acid-tolerant methanotrophs, the latter reinforced by the observation of maximum activity at pH = 4.5 with incubated slurries. A successful extraction of sterol and hopanoid lipids characteristic of methanotrophs was achieved, and their abundances greatly increased with increased sediment methane consumption. DNA extracted from methane-oxidizing enrichment cultures was amplified and sequenced for pmoA genes that aligned with methanotrophic members of the Gammaproteobacteria. An enrichment culture was established that grew in an acidic (pH 4.5) medium via methane oxidation.

Lack of methylated hopanoids renders the cyanobacterium Nostoc punctiforme sensitive to osmotic and pH stress

ABSTRACT To investigate the function of 2-methylhopanoids in modern cyanobacteria, the hpnP gene coding for the radical S-adenosyl methionine (SAM) methylase protein that acts on the C-2 position of hopanoids was deleted from the filamentous cyanobacterium Nostoc punctiforme ATCC 29133S. The resulting ΔhpnP mutant lacked all 2-methylhopanoids but was found to produce much higher levels of two bacteriohopanepentol isomers than the wild type. Growth rates of the ΔhpnP mutant cultures were not significantly different from those of the wild type under standard growth conditions. Akinete formation was also not impeded by the absence of 2-methylhopanoids. The relative abundances of the different hopanoid structures in akinete-dominated cultures of the wild-type and ΔhpnP mutant strains were similar to those of vegetative cell-dominated cultures. However, the ΔhpnP mutant was found to have decreased growth rates under both pH and osmotic stress, confirming a role for 2-methylhopanoids in stress tolerance. Evidence of elevated photosystem II yield and NAD(P)H-dependent oxidoreductase activity in the ΔhpnP mutant under stress conditions, compared to the wild type, suggested that the absence of 2-methylhopanoids increases cellular metabolic rates under stress conditions. IMPORTANCE As the first group of organisms to develop oxygenic photosynthesis, Cyanobacteria are central to the evolutionary history of life on Earth and the subsequent oxygenation of the atmosphere. To investigate the origin of cyanobacteria and the emergence of oxygenic photosynthesis, geobiologists use biomarkers, the remnants of lipids produced by different organisms that are found in geologic sediments. 2-Methylhopanes have been considered indicative of cyanobacteria in some environmental settings, with the parent lipids 2-methylhopanoids being present in many contemporary cyanobacteria. We have created a Nostoc punctiforme ΔhpnP mutant strain that does not produce 2-methylhopanoids to assess the influence of 2-methylhopanoids on stress tolerance. Increased metabolic activity in the mutant under stress indicates compensatory alterations in metabolism in the absence of 2-methylhopanoids.