Emma L. Duncan
Queensland University of Technology
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Featured researches published by Emma L. Duncan.
PLOS Genetics | 2011
Emma L. Duncan; Patrick Danoy; John P. Kemp; Paul Leo; Eugene McCloskey; Geoffrey C. Nicholson; Richard Eastell; Richard L. Prince; John A. Eisman; Graeme Jones; P. Sambrook; Ian R. Reid; Elaine M. Dennison; John D. Wark; J.B. Richards; A.G. Uitterlinden; Tim D. Spector; C. Esapa; Roger D. Cox; Steve D.M. Brown; Rajesh V. Thakker; K. Addison; Linda A. Bradbury; C Cooper; C. Cremin; Karol Estrada; Dieter Felsenberg; Claus-C. Glüer; Johanna Hadler; Margaret J. Henry
Osteoporotic fracture is a major cause of morbidity and mortality worldwide. Low bone mineral density (BMD) is a major predisposing factor to fracture and is known to be highly heritable. Site-, gender-, and age-specific genetic effects on BMD are thought to be significant, but have largely not been considered in the design of genome-wide association studies (GWAS) of BMD to date. We report here a GWAS using a novel study design focusing on women of a specific age (postmenopausal women, age 55–85 years), with either extreme high or low hip BMD (age- and gender-adjusted BMD z-scores of +1.5 to +4.0, n = 1055, or −4.0 to −1.5, n = 900), with replication in cohorts of women drawn from the general population (n = 20,898). The study replicates 21 of 26 known BMD–associated genes. Additionally, we report suggestive association of a further six new genetic associations in or around the genes CLCN7, GALNT3, IBSP, LTBP3, RSPO3, and SOX4, with replication in two independent datasets. A novel mouse model with a loss-of-function mutation in GALNT3 is also reported, which has high bone mass, supporting the involvement of this gene in BMD determination. In addition to identifying further genes associated with BMD, this study confirms the efficiency of extreme-truncate selection designs for quantitative trait association studies.
Journal of Bone and Mineral Research | 2006
John P. A. Ioannidis; Mandy Y.M. Ng; Pak Sham; Elias Zintzaras; Cathryn M. Lewis; Hong-Wen Deng; Michael J. Econs; David Karasik; Marcella Devoto; Candace M. Kammerer; Tim D. Spector; Toby Andrew; L. Adrienne Cupples; Emma L. Duncan; Tatiana Foroud; Douglas P. Kiel; Daniel L. Koller; Bente Langdahl; Braxton D. Mitchell; Munro Peacock; Robert R. Recker; Hui Shen; Katia Sol-Church; Loretta D. Spotila; André G. Uitterlinden; Scott G. Wilson; Annie W. C. Kung; Stuart H. Ralston
Several genome‐wide scans have been performed to detect loci that regulate BMD, but these have yielded inconsistent results, with limited replication of linkage peaks in different studies. In an effort to improve statistical power for detection of these loci, we performed a meta‐analysis of genome‐wide scans in which spine or hip BMD were studied. Evidence was gained to suggest that several chromosomal loci regulate BMD in a site‐specific and sex‐specific manner.
Journal of Bone and Mineral Research | 1999
Emma L. Duncan; Matthew A. Brown; Janet S Sinsheimer; John I. Bell; A J Carr; B P Wordsworth; Jah Wass
We have investigated the role of 23 candidate genes in the control of bone mineral density (BMD) by linkage studies in families of probands with osteoporosis (lumbar spine [LS] or femoral neck [FN] BMD T score < −2.5) and low BMD relative to an age‐ and gender‐matched cohort (Z score < −2.0). One hundred and fifteen probands (35 male, 80 female) and 499 of their first‐ or second‐degree relatives (223 males and 276 females) were recruited for the study. BMD was measured at the LS and FN using dual‐energy X‐ray absorptiometry and expressed as age‐ and gender‐matched Z scores corrected for body mass index. The candidate genes studied were the androgen receptor, type I collagen A1 (COLIA1), COLIA2, COLIIA1, vitamin D receptor (VDR), colony‐stimulating factor 1, calcium‐sensing receptor, epidermal growth factor (EGF), estrogen receptor 1 (ESR1), fibrillin type 1, insulin‐like growth factor 1, interleukin‐1 alpha (IL‐1α), interleukin‐4 (IL‐4), interleukin‐6 (IL‐6), interleukin‐11 (IL‐11), osteopontin, parathyroid hormone (PTH), PTH‐related peptide, PTH receptor type 1 (PTHR1), transforming growth factor‐beta 1, and tumor necrosis factors alpha and beta. Sixty‐four microsatellites lying close to or within these genes were investigated for linkage with BMD. Using the program MapMaker/Sibs there was suggestive evidence of linkage between BMD and PTHR1 (maximum LOD score obtained [MLS] 2.7–3.5). Moderate evidence of linkage was also observed with EGF (MLS 1.8), COLIA1 (MLS 1.7), COLIIA1/VDR (MLS 1.7), ESR1 (MLS 1.4), IL‐1α (MLS 1.4), IL‐4 (MLS 1.2), and IL‐6 (MLS 1.2). Variance components analysis using the program ACT, correcting for proband‐wise ascertainment, also showed evidence of linkage (p ≤ 0.05) at markers close to or within the candidate genes IL‐1α, PTHR1, IL‐6, and COLIIA1/VDR. Further studies will be required to confirm these findings, to refine the location of gene responsible for the observed linkage, and to screen the candidate genes targeted at these loci for mutations.
PLOS Genetics | 2012
Hou-Feng Zheng; Jon H Tobias; Emma L. Duncan; David Evans; Joel Eriksson; Lavinia Paternoster; Laura M. Yerges-Armstrong; Terho Lehtimäki; Ulrica Bergström; Mika Kähönen; Paul Leo; Olli T. Raitakari; Marika Laaksonen; Geoffrey C. Nicholson; Jorma Viikari; Martin Ladouceur; Leo-Pekka Lyytikäinen; Carolina Medina-Gomez; Fernando Rivadeneira; Richard L. Prince; Harri Sievänen; William D. Leslie; Dan Mellström; John A. Eisman; Sofia Movérare-Skrtic; David Goltzman; David A. Hanley; Graeme Jones; Beate St Pourcain; Yongjun Xiao
We aimed to identify genetic variants associated with cortical bone thickness (CBT) and bone mineral density (BMD) by performing two separate genome-wide association study (GWAS) meta-analyses for CBT in 3 cohorts comprising 5,878 European subjects and for BMD in 5 cohorts comprising 5,672 individuals. We then assessed selected single-nucleotide polymorphisms (SNPs) for osteoporotic fracture in 2,023 cases and 3,740 controls. Association with CBT and forearm BMD was tested for ∼2.5 million SNPs in each cohort separately, and results were meta-analyzed using fixed effect meta-analysis. We identified a missense SNP (Thr>Ile; rs2707466) located in the WNT16 gene (7q31), associated with CBT (effect size of −0.11 standard deviations [SD] per C allele, P = 6.2×10−9). This SNP, as well as another nonsynonymous SNP rs2908004 (Gly>Arg), also had genome-wide significant association with forearm BMD (−0.14 SD per C allele, P = 2.3×10−12, and −0.16 SD per G allele, P = 1.2×10−15, respectively). Four genome-wide significant SNPs arising from BMD meta-analysis were tested for association with forearm fracture. SNP rs7776725 in FAM3C, a gene adjacent to WNT16, was associated with a genome-wide significant increased risk of forearm fracture (OR = 1.33, P = 7.3×10−9), with genome-wide suggestive signals from the two missense variants in WNT16 (rs2908004: OR = 1.22, P = 4.9×10−6 and rs2707466: OR = 1.22, P = 7.2×10−6). We next generated a homozygous mouse with targeted disruption of Wnt16. Female Wnt16−/− mice had 27% (P<0.001) thinner cortical bones at the femur midshaft, and bone strength measures were reduced between 43%–61% (6.5×10−13<P<5.9×10−4) at both femur and tibia, compared with their wild-type littermates. Natural variation in humans and targeted disruption in mice demonstrate that WNT16 is an important determinant of CBT, BMD, bone strength, and risk of fracture.
Journal of Bone and Mineral Research | 2004
M. Audrey Koay; Py Woon; Yun Zhang; Lj Miles; Emma L. Duncan; Stuart H. Ralston; Juliet Compston; C Cooper; Richard Keen; Bente Langdahl; Alasdair MacLelland; Jeffrey O'Riordan; Huibert A. P. Pols; David M. Reid; André G. Uitterlinden; John Wass; Matthew A. Brown
Genetic studies based on cohorts with rare and extreme bone phenotypes have shown that the LRP5 gene is an important genetic modulator of BMD. Using family‐based and case‐control approaches, this study examines the role of the LRP5 gene in determining normal population variation of BMD and describes significant association and suggestive linkage between LRP5 gene polymorphisms and BMD in >900 individuals with a broad range of BMD.
American Journal of Human Genetics | 2013
Jan Halbritter; Albane A. Bizet; Miriam Schmidts; Jonathan D. Porath; Daniela A. Braun; Heon Yung Gee; Aideen McInerney-Leo; Pauline Krug; Emilie Filhol; Erica E. Davis; Rannar Airik; Peter G. Czarnecki; Anna Lehman; Peter Trnka; Patrick Nitschke; Christine Bole-Feysot; Markus Schueler; Bertrand Knebelmann; Stéphane Burtey; Attila J. Szabó; Kalman Tory; Paul Leo; Brooke Gardiner; Fiona McKenzie; Andreas Zankl; Matthew A. Brown; Jane Hartley; Eamonn R. Maher; Chunmei Li; Michel R. Leroux
Intraflagellar transport (IFT) depends on two evolutionarily conserved modules, subcomplexes A (IFT-A) and B (IFT-B), to drive ciliary assembly and maintenance. All six IFT-A components and their motor protein, DYNC2H1, have been linked to human skeletal ciliopathies, including asphyxiating thoracic dystrophy (ATD; also known as Jeune syndrome), Sensenbrenner syndrome, and Mainzer-Saldino syndrome (MZSDS). Conversely, the 14 subunits in the IFT-B module, with the exception of IFT80, have unknown roles in human disease. To identify additional IFT-B components defective in ciliopathies, we independently performed different mutation analyses: candidate-based sequencing of all IFT-B-encoding genes in 1,467 individuals with a nephronophthisis-related ciliopathy or whole-exome resequencing in 63 individuals with ATD. We thereby detected biallelic mutations in the IFT-B-encoding gene IFT172 in 12 families. All affected individuals displayed abnormalities of the thorax and/or long bones, as well as renal, hepatic, or retinal involvement, consistent with the diagnosis of ATD or MZSDS. Additionally, cerebellar aplasia or hypoplasia characteristic of Joubert syndrome was present in 2 out of 12 families. Fibroblasts from affected individuals showed disturbed ciliary composition, suggesting alteration of ciliary transport and signaling. Knockdown of ift172 in zebrafish recapitulated the human phenotype and demonstrated a genetic interaction between ift172 and ift80. In summary, we have identified defects in IFT172 as a cause of complex ATD and MZSDS. Our findings link the group of skeletal ciliopathies to an additional IFT-B component, IFT172, similar to what has been shown for IFT-A.
Reviews in Molecular Biotechnology | 2002
Jörg Dennig; Emma L. Duncan
The development of efficient methods to transfer genes into eukaryotic cells is important for molecular biotechnology. A number of different technologies to mediate gene transfer have been developed over the last 35 years, but most have drawbacks such as cytotoxicity, low efficiency and/or restricted applicability. Activated polyamidoamine (PAMAM)-dendrimers provide a new technology for gene transfer that offers significant advantages over classical methods. Reagents based on this technology provide high gene transfer efficiencies, minimal cytotoxicity, and can be used with a broad range of cell types. This technology could also be useful for in vivo gene transfer in gene therapy applications.
Arthritis & Rheumatism | 2009
Stuart I. Davidson; Xin Wu; Yu Liu; Meng Wei; Patrick Danoy; Gethin P. Thomas; Qing Cai; Linyun Sun; Emma L. Duncan; Niansong Wang; Qinghong Yu; Anlong Xu; Yonggui Fu; Matthew A. Brown; Huji Xu
OBJECTIVE The results of a recent genome-wide association study have shown that ERAP1 and IL23R are associated with ankylosing spondylitis (AS) in Caucasian populations from North America and the UK. Based on these findings, we undertook the current study to investigate whether single-nucleotide polymorphisms (SNPs) covering the genes ERAP1 and IL23R are associated with AS in a Han Chinese population. METHODS A case-control study was performed in Han Chinese patients with AS (n = 527) and controls (n = 945) from Shanghai and Nanjing. All patients met the modified New York criteria for AS. The Sequenom iPlex platform was used to genotype cases and controls for 21 tag SNPs covering IL23R and 38 tag SNPs covering ERAP1. Statistical analysis was performed using the Cochran-Armitage test for trend. RESULTS Multiple SNPs in ERAP1 were significantly associated with AS (for rs27980, P = 0.0048; for rs7711564, P = 0.0081). However, no association was observed between IL23R and AS (for all SNPs, P > 0.1). The nonsynonymous SNP in IL23R, rs11209026, widely thought to be the primary AS-associated SNP in IL23R in Europeans, was found not to be polymorphic in Chinese. CONCLUSION Our results demonstrate that genetic polymorphisms in ERAP1 are associated with AS in Han Chinese, suggesting a common pathogenic mechanism for the disease in Chinese and Caucasian populations, and that IL23R is not associated with AS in Chinese, indicating a difference in the mechanism of disease pathogenesis between Chinese and Caucasian populations. This may result from the fact that rs11209026, the nonsynonymous SNP in IL23R, is not polymorphic in Chinese patients, providing further evidence that rs11209026 is the key polymorphism associated with AS (and likely inflammatory bowel disease and psoriasis) in this gene.
Journal of Bone and Mineral Research | 2003
Emma L. Duncan; Lon R. Cardon; Janet S Sinsheimer; John Wass; Matthew A. Brown
Differences in genetic control of BMD by skeletal sites and genders were examined by complex segregation analysis in 816 members of 147 families with probands with extreme low BMD. Spine BMD correlated more strongly in male‐male comparisons and hip BMD in female‐female comparisons, consistent with gender‐ and site‐specificity of BMD heritability.
Oncogene | 1998
Sunil C. Kaul; Emma L. Duncan; Anna Englezou; Syuichi Takano; Roger R. Reddel; Youji Mitsui; Renu Wadhwa
The murine mortalin genes, mot-1 and mot-2, are members of the hsp70 family of proteins and differ from each other by only two amino acid residues. Mot-1 is expressed in normal cells and has pancytosolic cellular distribution whereas mot-2 is found in the perinuclear region of immortal cells. We report here that a high level of expression of mot-2 protein resulted in malignant transformation of cells as analysed by anchorage independent growth and nude mice assays. A high level of protein expression is attributed to the 900 bp 3′ untranslated region of the cDNA which does not have any transforming activity per se. Mortalin cDNA clones isolated from human transformed cells were also found to have transforming activity in similar assays and a high level of expression was apparent in some of the human immortalized cells that showed non-pancytosolic mortalin immunofluorescence. Taken together, the data suggest that nonpancytosolic mortalin may have a role in tumorigenesis.