Emrah Hicazi Aksu

Mitigation of paracetamol-induced reproductive damage by chrysin in male rats via reducing oxidative stress.

Paracetamol (PRC) is a nonsteroidal anti‐inflammatory drug used widely as a painkiller for various diseases and as the symptomatic flu cure in several countries worldwide. PRC toxicity may occur under conditions of the overdose usage. Chrysin (CR) is a flavonoid that is naturally present in several plants, honey and propolis. The aim of this study was to investigate the effects of CR (at the doses of 25 mg kg−1 and 50 mg kg−1) pre‐treatment over seven consecutive days against PRC‐induced reproductive toxicity in male rats. Our results showed that PRC toxicity decreased the sperm motility, and increased dead sperm rate, abnormal sperm cell rate, apoptosis and MDA levels in testicular tissues. Pre‐treatment with CR at the dose of 25 and 50 mg kg−1 for 7 days mitigated side effects of acute PRC toxicity in male reproductive system proportionally in a dose‐dependent manner. This possible protection mechanism might be dependent on the antioxidant activity of CR. In conclusion, pre‐treatment with CR at the dose of 25 and 50 mg kg−1 for 7 days can be the beneficial against PRC‐induced reproductive toxicity proportionally in a dose‐dependent manner.

Rutin ameliorates cisplatin-induced reproductive damage via suppression of oxidative stress and apoptosis in adult male rats.

Cisplatin (CP) treatment causes damage in the male reproductive system. Rutin (RUT) is a naturally occurring flavonoid glycoside that has antioxidant and anti‐inflammatory properties. This study aimed to investigate effects of RUT against cisplatin‐induced reproductive toxicity in male rats. Twenty‐one adult male Sprague Dawley rats were used. The control group received physiological saline with oral gavage during 14 days, and physiological saline was injected intraperitoneally (IP) in 10th days of study. CP Group received physiological saline during 14 days, and 10 mg kg−1 CP was injected IP in 10th day. RUT + CP group received RUT (150 mg kg−1) during 14 days, and 10 mg kg−1 CP was injected IP in 10th day. Spermatological parameters (including motility, cauda epididymal sperm density, dead sperm percentage and morphological sperm abnormalities), biochemical (MDA, GSH, GSH‐px, SOD and CAT), histological (H&E dye) and immunochemistry evaluations of testicles were evaluated. CP treatment caused damage on some spermatological parameters, increased the oxidative stress and induced testicular degeneration and apoptosis when compared to the control group. However, RUT treatment mitigates these side effects when compared to the CP alone group. IT is concluded that RUT treatment may reduce CP‐induced reproductive toxicity as a potential antioxidant compound.

Ameliorative Effect of Carvacrol on Cisplatin-Induced Reproductive Damage in Male Rats.

Cisplatin (CP) treatment causes the damage in male reproductive system. Carvacrol (CARV) is an antioxidant that is naturally found in some plants. We aimed to investigate the effect of CARV on CP‐induced reproductive toxicity in male rats. Eighteen adult male Sprague–Dawley rats were used. The control group (n = 6) was treated orally with physiological saline (PS) daily for 14 days and a single intraperitoneal (IP) PS injection on day 10. The CP group (n = 6) was administered with daily oral PS for 14 days and a single IP injection of 10 mg/kg CP on day 10. The CARV + CP group (n = 6) was treated with daily 75 mg/kg oral CARV for 14 days and a single IP injection of 10 mg/kg CP on day 10. CP treatment caused the damage on some spermatological parameters (motility, live sperm rate, and abnormal sperm rate), increased the oxidative stress, and induced testicular degeneration and apoptosis. However, CARV treatment mitigates CP‐induced reproductive toxicity.

Improvement in colistin-induced reproductive damage, apoptosis, and autophagy in testes via reducing oxidative stress by chrysin: AKSU et al.

This study aimed to investigate the effect of chrysin on colistin‐induced reproductive toxicity. Twenty‐eight adult male Sprague‐Dawley rats were divided into four groups of seven rats each. Group I received physiological saline for 7 days. Group II received 50 mg/kg/day chrysin for 7 days. Group III received a total dose of 73 mg/kg colistin for 7 days. Group IV received 50 mg/kg/day chrysin by an oral gavage after the colistin treatment. Colistin causes an increase in oxidative stress (OS) in the testis. Chrysin treatment significantly decreased the OS in the chrysin + colistin group compared with the colistin group. The highest caspase‐3 and LC3B expression levels were found in the colistin group and these levels were statistically lower in the chrysin + colistin group. Colistin treatment caused a decrease in sperm motility and an increase in sperm abnormality. Chrysin treatment mitigated these side effects significantly. In conclusion, chrysin treatment can be beneficial against colistin‐induced reproductive toxicity.

Geçiş Dönemindeki Esmer Irkı İneklerin Yavru Cinsiyetine Bağlı olarak Bazı Mineral, Hormonal ve Metabolik Parametrelerinin Karşılaştırılması

Sigirlarda gecis donemi dogum oncesindeki 3 hafta ile dogum sonrasi 3 haftayi kapsayan bir donem olarak tanimlanmaktadir. Bu calisma erkek ve disi yavru tasiyan esmer irki sigirlarin periparturient donemdeki bazi mineral, hormonal ve metabolik kan parametrelerinin karsilastirilmasi amaciyla yapilmistir. Calismada 20 gebe esmer irki inek (4-5 yaslarinda, 500-550 kg agirlikta) kullanilmistir. Ineklerden tahmin edilen dogum oncesi 21. gunde kan ornegi alinmistir. Dogum sonrasindaki 21. gunde bu ineklerden rasgele secilen 9 inegin kan numunesi kontrol (K) grubu olarak kullanilmistir. Kan serumlarinda glikoz, ure, kolesterol, kreatinin, bazi mineral maddeler ve FSH, LH ve progesteron (P4) hormon duzeyleri incelenmistir. 11 inegin erkek yavru 9 inegin de disi yavru dogurdugu dogumla teyit edilmistir. Dogum oncesi 21. gundeki ure seviyesi erkek yavru tasiyan (EYA) annelerde disi yavru tasiyan (DYA) anneler ve dogum sonrasi 21. gunde kan ornegi alinan K grubundakilere gore onemli derecede yuksek bulunmustur (P<0.05). EYA grubundaki kalsiyum (Ca) duzeyi K grubuna gore onemli derecede yuksek bulunmustur (P<0.05). Diger taraftan EYA ve DYA gruplarindaki glikoz, sodyum (Na), klor (Cl) ve P4 seviyesi K grubundan onemli derecede yuksek tespit edilmesine ragmen, kolesterol seviyesi ise onemli derece dusuk bulunmustur (P<0.05). Bununla birlikte kreatinin, potasyum (K), fosfor (P), magnezyum (Mg), demir (Fe), FSH ve LH duzeyleri acisinda her 3 grup arasinda da anlamli bir fark gozlenmemistir.

3,3 diindolylmethane leads to apoptosis, decreases sperm quality, affects blood estradiol 17 β and testosterone, oestrogen (α and β) and androgen receptor levels in the reproductive system in male rats

3,3 Diindolylmethane (DIM) is a major digestive product of indole‐3 carbinol, obtained from Brassica family vegetables such as broccoli, cabbage and Brussels sprouts. This study aimed to investigate the effects of DIM on sperm parameters, histological structures of testicular tissues, blood testosterone (T) and estradiol 17‐β (E2) in male rats.