Ender Erdoğan

Comparison of techniques for long-term storage of fat grafts: an experimental study.

Background: Absorption of autologous fat graft in the recipient area necessitates recurrent fat transplantation. Harvesting extra tissue during the first operation and storing it for future use is considered a solution to this problem. Methods: Fat tissue was removed from the inguinal region of 40 Swiss albino mice, which were arranged into four equal groups, and treated as follows: immediately transplanted to the donor animal; dry-frozen; immersed in glycerol; or frozen in liquid nitrogen. The grafts that were frozen or immersed in glycerol were stored at –35°C for 6 months and then transplanted to their original donors. Transplantations were performed by injecting the fat under the scalp. Viability of the fat tissue was evaluated with the MTT reduction test before transplantation, and histology of the transplanted tissue was examined at the end of the study. Results: The viability and histology of grafts frozen in liquid nitrogen were similar to those of fresh tissue, whereas with other methods the grafts had a considerable loss of viability during storage that was reflected in the low number of adipocytes and high proportion of vacuolar and fibrotic areas. Conclusion: Freezing fat grafts in liquid nitrogen and storing them at –35°C is an effective way of preserving tissue for future use, with clear superiority over other methods.

THE RELATIONSHIPS OF DOG HIPPOCAMPUS TO SEX AND PAW PREFERENCE

Previous studies have described paw preference and asymmetry in dog brains. Electrical activity of the dorsal hippocampus also indicated the existence of hippocampal asymmetry in dogs. In the present study, the possible paw and sex-related asymmetries and right-left differences in dog hippocampus were investigated. The hippocampus was dissected and weighed. Each hippocampus was cut into slices by the slicing apparatus placed horizontally on the tissues. The volumetric measurements were performed using the formula modified from the Cavalieri principle. The present study indicated the significant sex and paw differences and no right-left asymmetry in dog hippocampi. The morphological asymmetries in normal subjects might be related to functional hippocampal asymmetries in memory or in cognitive skills.

Consequences of Neurite Transection In Vitro

In order to quantify degenerative and regenerative changes and analyze the contribution of multiple factors to the outcome after neurite transection, we cultured adult mouse dorsal root ganglion neurons, and with a precise laser beam, we transected the nerve fibers they extended. Cell preparations were continuously visualized for 24 h with time-lapse microscopy. More distal cuts caused a more elongated field of degeneration, while thicker neurites degenerated faster than thinner ones. Transected neurites degenerated more if the uncut neurites of the same neuron simultaneously degenerated. If any of these uncut processes regenerated, the transected neurites underwent less degeneration. Regeneration of neurites was limited to distal cuts. Unipolar neurons had shorter regeneration than multipolar ones. Branching slowed the regenerative process, while simultaneous degeneration of uncut neurites increased it. Proximal lesions, small neuronal size, and extensive and rapid neurite degeneration were predictive of death of an injured neuron, which typically displayed necrotic rather than apoptotic form. In conclusion, this in vitro model proved useful in unmasking many new aspects and correlates of mechanically-induced neurite injury.

Hepatoprotective effects of coriandrum sativum essential oil against acute hepatotoxicity induced by carbon tetrachloride on rats

*Corresponding author: Hanefi Özbek E-mail address: hozbek@medipol.edu.tr 1 Istanbul Medipol University, School of Medicine, Department of Pharmacology, Istanbul 2Istanbul Medipol University, Vocational School of Health Services, Istanbul 3Sakarya University, Faculty of Medicine, Department of Histology-Embriology, Sakarya 4Selçuk University, Faculty of Medicine, Department of Histology-Embriology, Konya