Erbug Keskin

Antioxidant Effects of Probiotics in Experimentally Induced Peritonitis

AIM An experimental study was performed to evaluate the protective effects of probiotics on gut mucosa in peritonitis through antioxidant mechanisms. METHODS Thirty-two male Wistar albino rats were divided equally into four groups. The rats in Group 1 (control group) underwent laparotomy only. In group 2 (peritonitis group), peritonitis was induced in the rats by the cecal ligation and puncture (CLP) model. In group 3, the rats were treated with probiotics for five days after CLP-induced peritonitis. The last group of rats (group 4) were fed probiotics for five days before the CLP procedure and five days after the surgery. On the fifth day after surgery, all rats were killed, and tissue samples from the terminal ileum were obtained to evaluate the activities of myeloperoxidase (MPO), malondialdehyde (MDA), and glutathione (GSH). Histopathologic examinations were also performed to evaluate the grade of intestinal injury. RESULTS Myeloperoxidase and MDA activities were increased, GSH concentrations were decreased in group 2, compared with group 1. Intestinal MPO activities in group 4 were decreased compared with group 1 and group 2, indicating a reduction in oxidant activity. Malondialdehyde decreased in group 3 and decreased even more in group 4, compared with the peritonitis group (group 2). Glutathione concentrations were increased in group 4 compared with group 2 and group 3 (p < 0.05). The Chiu scores of the probiotics groups, groups 3 and 4, were lower than those in group 2, indicating reduced mucosal damage in the probiotically fed groups. CONCLUSION Probiotics have protective effects in peritonitis, which may be related to antioxidant mechanisms. This antioxidant effect of probiotics might occur when pre-conditioning with probiotics before peritonitis because there is sufficient time to prepare the tissues for oxidative damage.

Small Bowel Obstruction due to Anomalous Congenital Bands in Children

Introduction. The aim of the study was to evaluate our children who are operated on for anomalous congenital band while increasing the awareness of this rare reason of intestinal obstruction in children which causes a diagnostic challenge. Patients and Methods. We retrospectively reviewed the records of fourteen children treated surgically for intestinal obstructions caused by anomalous congenital bands. Results. The bands were located between the following regions: the ascending colon and the mesentery of the terminal ileum in 4 patients, the jejunum and mesentery of the terminal ileum in 3 patients, the ileum and mesentery of the terminal ileum in 2 patients, the ligament of Treitz and mesentery of the jejunum in one patient, the ligament of Treitz and mesentery of the terminal ileum in one patient, duodenum and duodenum in one patient, the ileum and mesentery of the ileum in one patient, the jejunum and mesentery of the jejunum in one patient, and Meckels diverticulum and its ileal mesentery in one patient. Band excision was adequate in all of the patients except the two who received resection anastomosis for intestinal necrosis. Conclusion. Although congenital anomalous bands are rare, they should be considered in the differential diagnosis of patients with an intestinal obstruction.

Isolated polyarteritis nodosa of the gallbladder in older children

A 10-year-old-boy with acute acalculous cholecystitis due to polyarteritis nodosa of the gallbladder with limited involvement of this organ is reported. It is suggested that a search for other lesions is indicated when polyarteritis nodosa of the gallbladder has been diagnosed, but that no treatment is indicated when additional lesions are not found.

The Role of Ozone Therapy in Hepatic Fibrosis due to Biliary Tract Obstruction

PURPOSES In this experimental study, we investigated the role of ozone therapy in hepatic fibrosis caused by biliary obstruction. MATERIALS AND METHODS In this study, 21 male Sprague-Dawley rats were divided equally into three groups. In the control group, only laparotomy was performed and intraperitoneal cavity is washed with 1 mL of saline. In the sham group (SG), common bile duct is binded with laparotomy and no treatment is given afterward. In the experimental group (EG) after the binding of common bile duct with laparotomy, 1 mg/kg dose and 50 mg/mL concentration of ozone were applied rectally for 21 days. Hepatic tissue sample and intracardiac blood samples were collected from all animals at postoperative 21st day with relaparatomy. RESULTS When we compared the experiment to SG, we detected a decrease in aspartate aminotransferase, alkaline phosphate (ALP), total bilirubin, and direct bilirubin levels in the EG, however, only the decrease in total bilirubin levels were statistically significant (p = 0.025). Histopathological examination of livers of rats in the EG showed lower level of hepatic fibrosis and inflammation. In the SG, incomplete cirrhosis was detected in 57.1% of the rats, whereas in the EG, no cirrhosis was detected. Immunohistochemically, periportal inflammation was 100% in the SG, whereas it was seen (3/7) 42.9% in the EG. A significant decrease in positive α-smooth muscle actin reaction was observed in ozone-treated group compared with SG. CONCLUSION We suggest that ozone can decrease the hepatic destruction levels in experimental model of biliary obstruction.

Long-term outcomes of children with transanal endorectal pull-through and a review of the literature.

Abstract Introduction: The transanal endorectal pull-through (TERPT) procedure, the latest advancement in the surgical treatment of Hirschsprung’s disease, has replaced most other surgical techniques in the last decade. Patients and methods: Between October 2002 and March 2014, a total of 22 patients diagnosed with Hirschsprung’s disease underwent a one-stage TERPT operation. Results: Resected segments included the rectosigmoid (seven patients), the descending colon (10 patients), and the transverse colon (five patients). The minimum length of the resected segments was 15 cm and the maximum length was 65 cm. The mean length was 39.18 ± 12.05 cm. Following surgery, the start of oral ingestion was 1–8 days (mean 3 ± 1.69 days) and the hospital stay after the operation lasted 4–11 days (mean 7.04 ± 2.05 days). The mean follow-up period was 48 ± 6 months (range of 24–166 months). Out of 22 patients, three patients had an anal stricture, which responded to anal dilatations; three patients had an enterocolitis episode that required hospitalization; two patients experienced constipation; and two patients had incontinence/soiling. Conclusion: Our data suggest that the TERPT operation can be safely performed in terms of long-term complications.

Is there a determining factor that predicts mortality in patients with congenital diaphragmatic hernia

Aim This study was designed to investigate the factors affecting the prognosis in neonates with congenital diaphragmatic hernia (CDH) who were treated in our clinic. These factors included prenatal lung-head ratio (LHR), prenatal stomach and liver presence in the thorax, blood gases in the first 24 h and the modified ventilation index (MVI). Material and methods The study was carried out retrospectively in 30 neonates with prenatally diagnosed left CDH who were treated in our clinic between January 2007 and 2013. Data were collected, evaluated, and statistically analyzed for gender, birth weight, gestational age, prenatal LHR, prenatal presence of stomach and liver in the thorax, postnatal initial blood gases in the first 24 h and MVI. Results The median LHR for non-survivors was 1.49 and for survivors 1.51. No statistically significant difference in LHR was detected between survivors and non-survivors. In 19 neonates, prenatal ultrasonography (USG) revealed intrathoracic stomach, and 9 of these infants died. Intrathoracic liver was seen in 15 neonates, and 9 of these died. A statistically significant difference was not found between survivors and non-survivors in the intrathoracic liver or intrathoracic stomach neonates. A comparison between the non-survivors and survivors showed a median pH value of 7.10 in non-survivors and 7.24 in survivors (p = 0.002). The median PaCO2 value was 69.4 mm Hg in non-survivors and 51.9 mm Hg in survivors (p = 0.01). There were statistically significant differences in pH and PaCO2 values. The median value of MVI was 33 in survivors and 100 in non-survivors. There was a statistically significant difference between overall non-survivors and survivors in the MVI value (p < 0.05). Conclusions Based on the findings, postnatal pH, and PaCO2 and MVI values are favorable prognostic factors in CDH in our selected group of patients.

Early myotomy and fundoplication in achalasia in childhood: a single-centre experience for 22 years

Abstract Introduction The aim of this study was to review a single institution’s experience with surgical interventions in children with achalasia and to determine treatment strategies for this rare disorder. Patients and methods This study is a retrospective analysis of 22 cases of childhood achalasia from 1991 to 2013. The patients were evaluated in terms of age, symptoms, interventions, intraoperative complications, and recurrent dysphagia. Results There were 13 boys and nine girls (7 months to 17 years old). The clinical symptoms were vomiting (68%), dysphagia (36%), wheezing (18%), coughing (13%), and weight-loss (13%). The mean duration of symptoms was 2.4 years (1 month to 6 years). A barium contrast X-ray study was performed in all of the patients. Oesophageal manometry was performed in eight patients. Six patients underwent multiple oesophageal dilatations (ED) as a first intervention. A Heller myotomy (HM) and fundoplication were performed in all the patients except two patients who recovered with dilatation. In the long term, one patient had a stricture due to the operation and had to undergo a reoperation. Of the Heller myotomy patients, one had a recurrent stricture that responded to dilatation. No other complications were present. All the patients are now asymptomatic. Conclusion Early diagnosis and prompt surgical treatment is important to prevent growth impairment in childhood achalasia cases. A Heller myotomy followed by a partial anti-reflux procedure is an effective treatment for achalasia in children. Based on our experience, it is superior to oesophageal dilatation therapy.