Ergi Deniz Özsoy

Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress

Heat shock proteins (HSPs), originally identified as heat-inducible gene products, are a family of highly conserved proteins that respond to a wide variety of stress including oxidative stress. Although both acute and chronic oxidative stress have been well demonstrated to induce HSP responses, little evidence is available whether increased HSP levels provide enhanced protection against oxidative stress under elevated yet sublethal temperatures. We studied relationships between oxidative stress and HSPs in a physiological model by using Garra rufa (doctor fish), a fish species naturally acclimatized to different thermal conditions. We compared fish naturally living in a hot spring with relatively high water temperature (34.4±0.6 °C) to those living in normal river water temperature (25.4±4.7 °C), and found that levels of all the studied HSPs (HSP70, HSP60, HSP90, HSC70 and GRP75) were higher in fish living in elevated water temperature compared with normal river water temperature. In contrast, indicators of oxidative stress, including protein carbonyls and lipid hydroperoxides, were decreased in fish living in the elevated temperature, indicating that HSP levels are inversely associated with oxidative stress. The present results provide evidence that physiologically increased HSP levels provide protection against oxidative stress and enhance cytoprotection.

Estimating Reaction Norms for Predictive Population Parameters, Age Specific Mortality, and Mean Longevity in Temperature-Dependent Cohorts of Culex quinquefasciatus Say (Diptera: Culicidae)

ABSTRACT: Culex quinquefasciatus plays a major role in the transmission of important parasites and viruses throughout the world. Because temperature is an important limiting factor on growth and longevity of all mosquito species, estimating the reaction norms provides very important basic information for understanding both plasticity and individual variations of the population. In the present study, Cx. quinquefasciatus were maintained at five different constant temperatures (15°, 20°, 23°, 27°, and 30°C) for two subsequent generations. Reproductive population parameters in blood-fed mated females and longevities of virgin and blood-fed mated adults reared at different temperatures were compared for the two generations. Longevity increased as temperature decreased within a range of 15° to 30°C for the unmated adults, and 15° to 27°C for the mated and blood-fed adults. Generation times were as long as 124.07 and 106.76 days for two subsequent generations reared at 15°C, and the highest intrinsic rate of increase (rm) values were estimated at 0.22 and 0.18, respectively, from the cohorts reared at 27°C. For survival rates, reproductive rates (R0), and rm values, 30°C was found to be a critical temperature for this species. These cohorts produced the smallest amount of eggs (R0= 5.06), rm values decreasing across generations (from 0.11 to 0.06), and the survival rates from egg to adult were found to be insufficient (16.1 and 10.8%). Additionally, the rate of exponential increase with age and age specific mortalities (b) were calculated for the virgin cohorts. Age specific mortality rates increased as temperature decreased. The increase in mortality rates started to accelerate at 27°C and was more pronounced at 30°C, for both females and males. We estimated the coefficients of variation for the b values in which females have smaller coefficients than those of the males at all temperatures.

The Effects of Royal Jelly on Fitness Traits and Gene Expression in Drosophila melanogaster

Royal Jelly (RJ) is a product made by honey bee workers and is required for queen differentiation and accompanying changes in queen body size, development time, lifespan and reproductive output relative to workers. Previous studies have reported similar changes in Drosophila melanogaster in response to RJ. Here, we quantified viability, development time, body size, productivity, lifespan and genome wide transcript abundance of D. melanogaster reared on standard culture medium supplemented with increasing concentrations of RJ. We found that lower concentrations of RJ do induce significant differences in body size in both sexes; higher concentrations reduce size, increase mortality, shorten lifespan and reduce productivity. Increased concentrations of RJ also consistently lengthened development time in both sexes. RJ is associated with changes in expression of 1,581 probe sets assessed using Affymetrix Drosophila 2.0 microarrays, which were enriched for genes associated with metabolism and amino acid degradation. The transcriptional changes are consistent with alterations in cellular processes to cope with excess nutrients provided by RJ, including biosynthesis and detoxification, which might contribute to accelerated senescence and reduced lifespan.

Narrow-sense heritability of body size and its response to different developmental temperatures in Culex quinquefasciatus (Say 1923)

ABSTRACT: Body size is an important trait involved in overall fitness through its effects on mating success, fecundity, resource acquisition and mortality, and desiccation resistance. In this study, we raised inbred Culex quinquefasciatus mosquito cohorts at different developmental temperatures of 20°, 23°, and 27° C. As an indicator of the amount of genetic variation in body size, we estimated the narrow-sense heritability of body sizes defined as wing aspect ratios. Our results show that narrow-sense heritability of the body size increased as the developmental temperature increased. We also detected the presence of strong genotype-by-environment (G × E) interaction from low cross-environmental correlations. The body size of each temperature regime followed the general rule that higher temperatures produce smaller individuals. We suggest that the increase in genetic variation with increasing temperature might be due to an unleashing of the cryptic genetic variation of the putative genes affecting body size. We conclude that this increase in genetic variation tracking the environmental (developmental temperature) change could have considerable implications for the distribution and range expansion of Cx. quinquefasciatus, especially in warmer environments.

Genetic variation in an isolated population of the newly identified endemic gudgeon Gobio sakaryaensis (Osteichthyes: Cyprinidae)

We analysed the genetic variation of the gudgeon Gobio sakaryaensis (Teleostei: Cyprinidae), which is endemic to a subbasin which has been isolated from the Sakarya watershed since the late Pliocene for at least two million years. From the 8 allozyme loci studied, more than half show heterozygote deficiency. Based on tests of selective neutrality, we conclude that the levels of deficiency can be accounted for by genetic drift. Big differences in the frequency between allelic classes at individual loci, an apparent indication of fixation, support this inference. We suggest that hydrogeological isolation is the reason for the genome of the Gobio sakaryaensis population to have evolved mainly by genetic drift.