Erhard Roeder

Camptothecin and 10-hydroxycamptothecin in callus and plantlets of t Camptotheca acuminata

The process of callus induction, organogenesis and plantlets regeneration of Camptotheca acuminata Decne is reported. The highest growth rate of callus was observed on MS medium with 1 mg l−1 NAA, 1 mg l−1 kinetin and 60 g l−1 sucrose. All tissues and organs developed in vitro contain camptothecin and 10-hydroxycamptothecin. The presence of 10-hydroxycamptothecin in shoots and callus of Camptotheca acuminata Decne is reported for the first time. The alkaloids were detected and identified using HPLC methods.

Carbon-13 NMR spectroscopy of pyrrolizidine alkaloids

Abstract 13 C NMR chemical shifts of more than 130 naturally occurring alkaloids published up to 1988 are listed. The utility of 13 C NMR spectroscopy for the structure elucidation of these compounds combined with mass spectra is discussed.

Pyrrolizidine alkaloids of Heliotropium spathulatum

Abstract Isolation and structural determination of the pyrrolizidine alkaloids of Heliotropium spathulatum are reported. The alkaloids lycopsamine, amabiline, curassavinine, coromandalinine, heliovicine, coromandaline, curassavine and the two new pyrrolizidine alkaloids, heliospathine and heliospathuline were determined by spectroscopic methods. Traces of another new alkaloid were detected.

Pyrrolizidine alkaloids from Echium pininana

Abstract Seven pyrrolizidine alkaloids were isolated from Echium pininana and their structures determined by spectroscopic methods. One of them is new and the name echiupinine is proposed for it. The corresponding echiupinine-N-oxide and the previously undescribed myoscorpine-N-oxide are also present in this plant species.

Pyrrolizidine alkaloids from Senecio integrifolius var. Fauriri

Abstract A new water-soluble pyrrolizidine alkaloid quaternary salt, N -methyl- O 7 , O 9 -diangeloyl-1-hydroxyl platynecinium chloride, a new saturated pyrrolizidine alkaloid, O 7 -angeloylturneforcidine, a new saturated otonecine pyrrolizidine, 1,2-dihydrosenkirkine, a known alkaloid, O 7 -angeloylheliotridine and its N -oxide were isolated from Senecio integrifolius var. fauriri . The structures of the alkaloids and their stereochemistry have been elucidated by NMR analysis.

Pyrrolizidine alkaloids from Alkanna tinctoria

Abstract Three pyrrolizidine alkaloids were isolated from Alkanna tinctoria and their structures analysed by spectroscopic methods. One of them is new and the name dihydroxytriangularine is proposed for it.

Quantitative Photometric Determination of Senkirkine in Farfarae Folium

A colour reaction for unsaturated pyrrolizidine alkaloids is made applicable for the first time to otonecine alkaloids and is used for the photometric determination of senkirkine in coltsfoot. The method allows the determination of 1 μg of alkaloid in 1 g of dried drug (i.e. 1 p.p.m.) and is therefore a useful alternative to the more exacting gas chromatographic determination.

Pyrrolizidine alkaloids from Lithospermum erythrorhizon

Abstract Three pyrrolizidine alkaloids were isolated from Lithospermum erythrorhizon and their structures analysed by spectroscopical methods. One of them is new and the name hydroxymyoscorpine is proposed for it.

Pyrrolizidine alkaloids from seeds of Crotalaria scassellatii

Abstract Three pyrrolizidine alkaloids were isolated from the seeds of Crotalaria scassellatii . Axillaridine and axillarine were the two major alkaloids whereas the third minor alkaloid was a new compound. Its structure was determined as desoxyaxillarine.

Pyrrolizidine alkaloids from Senecio cacaliaster

Abstract Four pyrrolizidine alkaloids have been isolated from Senecio cacaliaster and their structures analysed by spectroscopic methods (IR, mass, 1 H, 13 CNMR). One of them is new and the name sencalenine (3) is proposed. Alkaloids O 7 -senecioylretronecine (1) and 7-senecioyl-9-sarracinylretronecine (2) have recently been identified elsewhere. The fourth is bulgarsenine (4) which was isolated from a Senecio species before.