Erica Seubert

USC CINAPS Builds Bridges

More than 70% of our earth is covered by water, yet we have explored less than 5% of the aquatic environment. Aquatic robots, such as autonomous underwater vehicles (AUVs), and their supporting infrastructure play a major role in the collection of oceanographic data. To make new discoveries and improve our overall understanding of the ocean, scientists must make use of these platforms by implementing effective monitoring and sampling techniques to study ocean upwelling, tidal mixing, and other ocean processes. Effective observation and continual monitoring of a dynamic system as complex as the ocean cannot be done with one instrument in a fixed location. A more practical approach is to deploy a collection of static and mobile sensors, where the information gleaned from the acquired data is distributed across the network. Additionally, orchestrating a multisensor, long-term deployment with a high volume of distributed data involves a robust, rapid, and cost-effective communication network. Connecting all of these components, which form an aquatic robotic system, in synchronous operation can greatly assist the scientists in improving our overall understanding of the complex ocean environment.

Examination of the Seasonal Dynamics of the Toxic Dinoflagellate Alexandrium catenella at Redondo Beach, California, by Quantitative PCR

ABSTRACT The presence of neurotoxic species within the genus Alexandrium along the U.S. coastline has raised concern of potential poisoning through the consumption of contaminated seafood. Paralytic shellfish toxins (PSTs) detected in shellfish provide evidence that these harmful events have increased in frequency and severity along the California coast during the past 25 years, but the timing and location of these occurrences have been highly variable. We conducted a 4-year survey in King Harbor, CA, to investigate the seasonal dynamics of Alexandrium catenella and the presence of a particulate saxitoxin (STX), the parent compound of the PSTs. A quantitative PCR (qPCR) assay was developed for quantifying A. catenella in environmental microbial assemblages. This approach allowed for the detection of abundances as low as 12 cells liter−1, 2 orders of magnitude below threshold abundances that can impact food webs. A. catenella was found repeatedly during the study, particularly in spring, when cells were detected in 38% of the samples (27 to 5,680 cells liter−1). This peak in cell abundances was observed in 2006 and corresponded to a particulate STX concentration of 12 ng liter−1, whereas the maximum STX concentration of 26 ng liter−1 occurred in April 2008. Total cell abundances and toxin levels varied strongly throughout each year, but A. catenella was less abundant during summer, fall, and winter, when only 2 to 11% of the samples yielded positive qPCR results. The qPCR method developed here provides a useful tool for investigating the ecology of A. catenella at subbloom and bloom abundances.

Algal toxins and reverse osmosis desalination operations: Laboratory bench testing and field monitoring of domoic acid, saxitoxin, brevetoxin and okadaic acid

The occurrence and intensity of harmful algal blooms (HABs) have been increasing globally during the past few decades. The impact of these events on seawater desalination facilities has become an important topic in recent years due to enhanced societal interest and reliance on this technology for augmenting world water supplies. A variety of harmful bloom-forming species of microalgae occur in southern California, as well as many other locations throughout the world, and several of these species are known to produce potent neurotoxins. These algal toxins can cause a myriad of human health issues, including death, when ingested via contaminated seafood. This study was designed to investigate the impact that algal toxin presence may have on both the intake and reverse osmosis (RO) desalination process; most importantly, whether or not the naturally occurring algal toxins can pass through the RO membrane and into the desalination product. Bench-scale RO experiments were conducted to explore the potential of extracellular algal toxins contaminating the RO product. Concentrations exceeding maximal values previously reported during natural blooms were used in the laboratory experiments, with treatments comprised of 50 μg/L of domoic acid (DA), 2 μg/L of saxitoxin (STX) and 20 μg/L of brevetoxin (PbTx). None of the algal toxins used in the bench-scale experiments were detectable in the desalinated product water. Monitoring for intracellular and extracellular concentrations of DA, STX, PbTx and okadaic acid (OA) within the intake and desalinated water from a pilot RO desalination plant in El Segundo, CA, was conducted from 2005 to 2009. During the five-year monitoring period, DA and STX were detected sporadically in the intake waters but never in the desalinated water. PbTx and OA were not detected in either the intake or desalinated water. The results of this study demonstrate the potential for HAB toxins to be inducted into coastal RO intake facilities, and the ability of typical RO operations to effectively remove these toxins.

Impact of Algal Blooms and Their Toxins on Reverse Osmosis Desalination Plant Operations

Abstract Seawater desalination by reverse osmosis (RO) has emerged over the past few decades as a reliable and necessary method for augmenting drinking water supplies in many parts of the world. As freshwater resources become limited due to global climate change, rising demand, and exhausted local water supplies, seawater desalination will play an increasingly important role in securing the world’s future water supply. The global increase in occurrence and incidence of harmful algae blooms is a concern for RO desalination plants due to the high biomass of microalgae present in ocean waters during these events, and a variety of deleterious substances that some algae produce. The compounds range from innocuous substances that can cause membrane fouling, to noxious compounds and powerful neurotoxins that constitute significant public health risks if they are not effectively and completely removed by RO membranes. Algal blooms can cause significant operational issues that can necessitate costly pretreatment procedures, result in increased energy usage due to membrane fouling, and in extreme cases, require a plant to be removed from service. Early algal bloom detection by desalination facilities is essential so that operational adjustments can be made to ensure that production capacity remains unaffected. This chapter will focus on characterizing a range of substances of concern to desalination facilities, their known producers, and our present state of knowledge regarding substances of particular concern due to their toxicity. Also included is a case study from southern California, which involved the monitoring of intake and desalinated water from a pilot SWRO desalination facility, and bench-top SWRO experiments modeled the worst-case scenario toxic events for the local area.

A decade and a half of Pseudo-nitzschia spp. and domoic acid along the coast of southern California

Blooms of the marine diatom genus Pseudo-nitzschia that produce the neurotoxin domoic acid have been documented with regularity along the coast of southern California since 2003, with the occurrence of the toxin in shellfish tissue predating information on domoic acid in the particulate fraction in this region. Domoic acid concentrations in the phytoplankton inhabiting waters off southern California during 2003, 2006, 2007, 2011 and 2017 were comparable to some of the highest values that have been recorded in the literature. Blooms of Pseudo-nitzschia have exhibited strong seasonality, with toxin appearing predominantly in the spring. Year-to-year variability of particulate toxin has been considerable, and observations during 2003, 2006, 2007, 2011 and again in 2017 linked domoic acid in the diets of marine mammals and seabirds to mass mortality events among these animals. This work reviews information collected during the past 15 years documenting the phenology and magnitude of Pseudo-nitzschia abundances and domoic acid within the Southern California Bight. The general oceanographic factors leading to blooms of Pseudo-nitzschia and outbreaks of domoic acid in this region are clear, but subtle factors controlling spatial and interannual variability in bloom magnitude and toxin production remain elusive.